Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,713,568	T→C	W225R (TGG→CGG)	srmB →	ATP‑dependent RNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,713,568	0	T	C	100.0%	125.1 / NA	35	W225R (TGG→CGG)	srmB	ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/17); total (18/17)

ACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGTGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAA  >  NC_000913/2713445‑2713691

aCCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACcg                                                                                                                   <  2:867809/134‑1 (MQ=255)

aCCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACcg                                                                                                                   >  1:867809/1‑134 (MQ=255)

aCCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCg                                                                                                              >  2:255306/1‑139 (MQ=255)

aCCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCg                                                                                                              >  1:742435/1‑139 (MQ=255)

aCCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCg                                                                                                              >  1:527005/1‑139 (MQ=255)

aCCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCg                                                                                                              <  1:544410/139‑1 (MQ=255)

                                  cgatTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                            <  2:527005/139‑1 (MQ=255)

                                            cTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTaaaa                                                                  >  2:343277/1‑139 (MQ=255)

                                               tGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAg                                                               >  1:609419/1‑139 (MQ=255)

                                                         ctgctgGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCa                                                                                                 <  2:613081/95‑1 (MQ=255)

                                                         ctgctgGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCa                                                                                                 >  1:613081/1‑95 (MQ=255)

                                                          tgctgGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGAc                                                    <  1:181961/139‑1 (MQ=255)

                                                                aaGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTc                                              <  1:255306/139‑1 (MQ=255)

                                                                                  cTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCgcgc                                                                                                                <  2:204517/55‑1 (MQ=255)

                                                                                  cTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCgcgc                                                                                                                >  1:204517/1‑55 (MQ=255)

                                                                                      cAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCTATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAagcgt                        <  2:820375/139‑1 (MQ=255)

                                                                                         tCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACcgc                                                                                                                  <  1:539454/46‑1 (MQ=255)

                                                                                         tCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACcgc                                                                                                                  >  2:539454/1‑46 (MQ=255)

                                                                                          cccTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAagcgtgagc                    <  2:742435/139‑1 (MQ=255)

                                                                                          cccTCCACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAagcgtgagc                    <  2:145643/139‑1 (MQ=255)

                                                                                               cACCCGTGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCCCAATTGTGTTTGTGCGTAAGCGTGAGCGtgtg               >  2:959101/1‑139 (MQ=255)

                                                                                                     tGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCtt                                                                                                      <  2:203690/46‑1 (MQ=255)

                                                                                                     tGAGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCtt                                                                                                      >  1:203690/1‑46 (MQ=255)

                                                                                                       aGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTc                                              <  2:254572/100‑1 (MQ=255)

                                                                                                       aGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTc                                              >  1:254572/1‑100 (MQ=255)

                                                                                                       aGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGt                              >  1:580162/1‑116 (MQ=255)

                                                                                                       aGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGt                              <  2:580162/116‑1 (MQ=255)

                                                                                                       aGCGCAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCt       >  1:973265/1‑139 (MQ=255)

                                                                                                         cgcAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                            <  2:352143/68‑1 (MQ=255)

                                                                                                         cgcAAAAAAATTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCa                                                                            >  1:352143/1‑68 (MQ=255)

                                                                                                            aaaaaaaTTCATCAGCGGTATTACCGCGCCGATGATCtt                                                                                                      <  2:115745/39‑1 (MQ=255)

                                                                                                            aaaaaaaTTCATCAGCGGTATTACCGCGCCGATGATCtt                                                                                                      >  1:115745/1‑39 (MQ=255)

                                                                                                            aaaaaaaTTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTc                                              >  2:19657/1‑95 (MQ=255)

                                                                                                            aaaaaaaTTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTc                                              <  1:19657/95‑1 (MQ=255)

                                                                                                            aaaaaaaTTCATCAGCGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCaa  >  1:517316/1‑139 (MQ=255)

ACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCCGAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGTGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAA  >  NC_000913/2713445‑2713691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A