Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	383,259	C→T	R226Q (CGG→CAG)	yaiP ←	putative family 2 glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	383,259	0	C	T	100.0%	207.6 / NA	64	R226Q (CGG→CAG)	yaiP	putative family 2 glycosyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/36); total (28/36)

ACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAAC  >  NC_000913/383123‑383387

aCCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGa                                                                                                                                >  1:1256529/1‑139 (MQ=255)

     aGCGGCTAAATAAAAGTCTTTTATTCAGGCGCATACAGACCGCGTATCCCACAATCCATCGCCGCCCGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACg                                                                                                                           >  2:655896/1‑139 (MQ=255)

     aGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGaa                                                                                                                               <  2:347357/135‑1 (MQ=255)

     aGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGaa                                                                                                                               >  1:347357/1‑135 (MQ=255)

     aGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACg                                                                                                                           >  1:867789/1‑139 (MQ=255)

     aGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACg                                                                                                                           >  1:752501/1‑139 (MQ=255)

       cGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGt                                                                                                                         <  2:147784/139‑1 (MQ=255)

        ggCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTa                                                                                                                        >  2:1015166/1‑139 (MQ=255)

         gCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAg                                                                                                                       >  1:1197241/1‑139 (MQ=255)

               taaaAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGttt                                                                                                                 <  2:1197241/139‑1 (MQ=255)

                     tCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacc                                                                                                           <  2:867789/139‑1 (MQ=255)

                      cTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                          <  1:1015166/139‑1 (MQ=255)

                       ttttATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCaa                                                                                                         >  1:1307792/1‑139 (MQ=255)

                         ttATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATg                                                                                                       >  1:189071/1‑139 (MQ=255)

                           aTGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTc                                                                                                     <  1:77893/139‑1 (MQ=255)

                           aTGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTc                                                                                                     <  1:1070040/139‑1 (MQ=255)

                           aTGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTc                                                                                                     <  2:729750/139‑1 (MQ=255)

                                 gcgcATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCNATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTg                                                                                               >  1:1054478/1‑139 (MQ=255)

                                 gcgcATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTg                                                                                               >  1:381237/1‑139 (MQ=255)

                                             cgcgTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCt                                                                                   >  2:650506/1‑139 (MQ=255)

                                               cgTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTACTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTc                                                                                 >  1:538648/1‑139 (MQ=255)

                                                        caATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGt                                                                        <  1:35023/139‑1 (MQ=255)

                                                         aaTCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTa                                                                       <  2:56459/139‑1 (MQ=255)

                                                             cAACGTCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGt                                                                                                <  1:842041/110‑1 (MQ=255)

                                                             cAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGt                                                                                                >  2:842041/1‑110 (MQ=255)

                                                             cAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGAt                                                                   >  2:558049/1‑139 (MQ=255)

                                                              aaCGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATc                                                                  >  1:629489/1‑139 (MQ=255)

                                                                     ccAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGaaa                                                           >  2:625903/1‑139 (MQ=255)

                                                                      cAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAAc                                                          <  2:381237/139‑1 (MQ=255)

                                                                           aCGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAAc                                                     <  2:1089/139‑1 (MQ=255)

                                                                              ccACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCTGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTa                                                  <  2:752501/139‑1 (MQ=255)

                                                                              ccACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTa                                                  <  1:43186/139‑1 (MQ=255)

                                                                              ccACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTa                                                  <  1:170832/139‑1 (MQ=255)

                                                                                aCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACg                                                <  2:629489/139‑1 (MQ=255)

                                                                                   cctcACGCGTGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCaa                                             <  2:902668/139‑1 (MQ=255)

                                                                                        cgcgGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACAt                                        <  2:669815/139‑1 (MQ=255)

                                                                                        cgcgGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACAt                                        <  1:937915/139‑1 (MQ=255)

                                                                                          cgGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCa                                      <  2:189071/139‑1 (MQ=255)

                                                                                            ggCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAAc                                                          <  1:434929/117‑1 (MQ=255)

                                                                                            ggCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAAc                                                          >  2:434929/1‑117 (MQ=255)

                                                                                             gCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTACTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTa                                   <  2:538648/139‑1 (MQ=255)

                                                                                                   gCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAAc                             <  1:391974/139‑1 (MQ=255)

                                                                                                       tCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTc                                                                                 >  1:1192701/1‑83 (MQ=255)

                                                                                                       tCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTc                                                                                 <  2:1192701/83‑1 (MQ=255)

                                                                                                         cTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                          <  1:491110/56‑1 (MQ=255)

                                                                                                         cTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                          >  2:491110/1‑56 (MQ=255)

                                                                                                         cTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCg                       >  1:479761/1‑139 (MQ=255)

                                                                                                            tgGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                          >  2:294654/1‑53 (MQ=255)

                                                                                                            tgGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                          <  1:294654/53‑1 (MQ=255)

                                                                                                              ggTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCa                                      >  2:275825/1‑119 (MQ=255)

                                                                                                              ggTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCa                                      <  1:275825/119‑1 (MQ=255)

                                                                                                               gTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCa                 <  1:650506/139‑1 (MQ=255)

                                                                                                               gTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCa                 <  2:805248/139‑1 (MQ=255)

                                                                                                                   aCGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCa                                      <  2:1296743/114‑1 (MQ=255)

                                                                                                                   aCGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCa                                      >  1:1296743/1‑114 (MQ=255)

                                                                                                                        gCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTa                                                                       >  2:1020417/1‑76 (MQ=255)

                                                                                                                        gCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTa                                                                       <  1:1020417/76‑1 (MQ=255)

                                                                                                                         cAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACa                                         <  2:963699/105‑1 (MQ=255)

                                                                                                                         cAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACa                                         >  1:963699/1‑105 (MQ=255)

                                                                                                                            cGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGAtaataa    >  1:501117/1‑139 (MQ=255)

                                                                                                                              aTTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAAc  <  2:479761/139‑1 (MQ=255)

                                                                                                                                 cGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTg                                                                                               <  2:511872/43‑1 (MQ=255)

                                                                                                                                 cGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTg                                                                                               >  1:511872/1‑43 (MQ=255)

                                                                                                                                 cGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACt                                                                             >  2:249595/1‑61 (MQ=255)

                                                                                                                                 cGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACt                                                                             <  1:249595/61‑1 (MQ=255)

ACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAAC  >  NC_000913/383123‑383387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A