Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	383,259	C→T	R226Q (CGG→CAG)	yaiP ←	putative family 2 glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	383,259	0	C	T	100.0%	169.2 / NA	52	R226Q (CGG→CAG)	yaiP	putative family 2 glycosyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/26); total (26/26)

AGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCGC  >  NC_000913/383128‑383394

aGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACg                                                                                                                                  >  1:450734/1‑139 (MQ=255)

   ggCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTa                                                                                                                               >  1:492857/1‑139 (MQ=255)

    gCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAg                                                                                                                              <  1:1020891/139‑1 (MQ=255)

            aaaGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGc                                                                                                                      <  1:1010996/139‑1 (MQ=255)

                 cTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                                 <  2:979615/139‑1 (MQ=255)

                 cTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                                 <  2:612919/139‑1 (MQ=255)

                  ttttATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCaa                                                                                                                >  1:195651/1‑139 (MQ=255)

                     tATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGt                                                                                                             <  2:769216/139‑1 (MQ=255)

                           ggCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGt                                                                                                       <  2:848634/139‑1 (MQ=255)

                           ggCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGt                                                                                                       <  2:60315/139‑1 (MQ=255)

                                      aCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                                 >  2:21745/1‑118 (MQ=255)

                                      aCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                                 <  1:419039/118‑1 (MQ=255)

                                      aCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                                 <  1:21745/118‑1 (MQ=255)

                                      aCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                                 >  2:419039/1‑118 (MQ=255)

                                      aCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTc                                                                                                            >  1:829383/1‑123 (MQ=255)

                                      aCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTc                                                                                                            <  2:829383/123‑1 (MQ=255)

                                      aCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGt                                                                                            >  1:832121/1‑139 (MQ=255)

                                      aCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGt                                                                                            >  2:496138/1‑139 (MQ=255)

                                         gcgTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCtt                                                                                         <  2:195651/139‑1 (MQ=255)

                                          cgTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTc                                                                                        >  1:308761/1‑139 (MQ=255)

                                             aTCCCACAATCCAACGCCGCCAGCGACGCCACTCC‑CACGCGGGCTGTTGTATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAAGGTCCAGGTGAGATCAAGGTCTTCGACt                                                                                    <  2:160042/139‑1 (MQ=255)

                                                    aaTCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTc                                                                                                            <  2:313761/109‑1 (MQ=255)

                                                    aaTCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTc                                                                                                            >  1:313761/1‑109 (MQ=255)

                                                                ccAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTTCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGaaa                                                                  >  2:719991/1‑139 (MQ=255)

                                                                ccAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAgg                                                                                                        >  1:81678/1‑101 (MQ=255)

                                                                ccAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAgg                                                                                                        <  2:81678/101‑1 (MQ=255)

                                                                ccAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGaaa                                                                  >  2:157633/1‑139 (MQ=255)

                                                                 cAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAAc                                                                 <  2:506487/139‑1 (MQ=255)

                                                                           aCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACg                                                       <  1:245068/139‑1 (MQ=255)

                                                                                                      gtgGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccac                                                                                                                   <  2:221769/52‑1 (MQ=255)

                                                                                                      gtgGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccac                                                                                                                   >  1:221769/1‑52 (MQ=255)

                                                                                                         ggTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGc                         >  2:965329/1‑139 (MQ=255)

                                                                                                             taCGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCg                     <  1:263779/139‑1 (MQ=255)

                                                                                                              aCGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACAt                                               >  1:485175/1‑112 (MQ=255)

                                                                                                              aCGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACAt                                               <  2:485175/112‑1 (MQ=255)

                                                                                                              aCGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGc                    <  2:450734/139‑1 (MQ=255)

                                                                                                              aCGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGc                    >  1:548385/1‑139 (MQ=255)

                                                                                                              aCGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCACAGGCACCGc                    <  2:700634/139‑1 (MQ=255)

                                                                                                                gATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTg                  >  1:129214/1‑139 (MQ=255)

                                                                                                                   gCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTc                                                                                        <  1:249535/66‑1 (MQ=255)

                                                                                                                   gCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTc                                                                                        >  2:249535/1‑66 (MQ=255)

                                                                                                                   gCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATAc                                                 >  2:736610/1‑105 (MQ=255)

                                                                                                                   gCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATAc                                                 <  1:736610/105‑1 (MQ=255)

                                                                                                                   gCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCa                        >  2:339847/1‑130 (MQ=255)

                                                                                                                   gCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCa                        <  1:339847/130‑1 (MQ=255)

                                                                                                                   gCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGata               >  1:198115/1‑139 (MQ=255)

                                                                                                                    cAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCaa                                                    <  1:676917/101‑1 (MQ=255)

                                                                                                                    cAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCaa                                                    >  2:676917/1‑101 (MQ=255)

                                                                                                                     aGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGataat             >  1:279031/1‑139 (MQ=255)

                                                                                                                       cGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATAc                                                 >  2:913248/1‑101 (MQ=255)

                                                                                                                       cGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATAc                                                 <  1:913248/101‑1 (MQ=255)

                                                                                                                            cGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGt      >  2:188770/1‑139 (MQ=255)

                                                                                                                                tGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTgcgc  <  1:593162/139‑1 (MQ=255)

AGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAACGGTGCGC  >  NC_000913/383128‑383394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A