Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	383,259	C→T	R226Q (CGG→CAG)	yaiP ←	putative family 2 glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	383,259	0	C	T	100.0%	190.0 / NA	59	R226Q (CGG→CAG)	yaiP	putative family 2 glycosyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (29/30); total (29/30)

AGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAAC  >  NC_000913/383128‑383387

aGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACg                                                                                                                           >  1:299097/1‑139 (MQ=255)

aGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACg                                                                                                                           >  2:617768/1‑139 (MQ=255)

                 cTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                          <  1:750715/139‑1 (MQ=255)

                 cTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                          <  1:774094/139‑1 (MQ=255)

                 cTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                          <  2:311999/139‑1 (MQ=255)

                           ggCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGt                                                                                                <  2:604015/139‑1 (MQ=255)

                           ggCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGt                                                                                                >  1:55520/1‑139 (MQ=255)

                                      aCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGaa                                                                                                                               <  1:104322/97‑1 (MQ=255)

                                      aCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGaa                                                                                                                               <  2:591398/97‑1 (MQ=255)

                                      aCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGaa                                                                                                                               >  2:104322/1‑97 (MQ=255)

                                      aCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGaa                                                                                                                               >  1:591398/1‑97 (MQ=255)

                                      aCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGt                                                                                     >  1:792737/1‑139 (MQ=255)

                                      aCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGt                                                                                     >  1:223591/1‑139 (MQ=255)

                                      aCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGt                                                                                     >  2:731027/1‑139 (MQ=255)

                                      aCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGt                                                                                     >  2:307551/1‑139 (MQ=255)

                                        cgcgTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCt                                                                                   >  2:616985/1‑139 (MQ=255)

                                         gcgTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCtt                                                                                  <  2:391300/139‑1 (MQ=255)

                                          cgTATCCCACAATCCAACGCCGCTAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTc                                                                                 <  1:328485/139‑1 (MQ=255)

                                          cgTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                          <  2:652613/114‑1 (MQ=255)

                                          cgTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGccacca                                                                                                          >  1:652613/1‑114 (MQ=255)

                                          cgTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGc                                                                                                               >  2:346343/1‑109 (MQ=255)

                                          cgTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGc                                                                                                               <  1:346343/109‑1 (MQ=255)

                                          cgTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTc                                                                                 >  1:112920/1‑139 (MQ=255)

                                           gTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCg                                                                                >  2:723918/1‑139 (MQ=255)

                                           gTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCg                                                                                <  2:796198/139‑1 (MQ=255)

                                                    aaTCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTa                                                                       <  1:731027/139‑1 (MQ=255)

                                                    aaTCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTa                                                                       <  1:723918/139‑1 (MQ=255)

                                                         aaCGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATc                                                                  >  2:747745/1‑139 (MQ=255)

                                                         aaCGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATc                                                                  >  1:178624/1‑139 (MQ=255)

                                                            gccgccAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCga                                                               >  2:588472/1‑139 (MQ=255)

                                                              cgccAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTc                                                                                 >  1:131935/1‑119 (MQ=255)

                                                              cgccAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTc                                                                                 <  2:131935/119‑1 (MQ=255)

                                                                ccAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGaaa                                                           >  1:681331/1‑139 (MQ=255)

                                                                 cAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAAc                                                          <  1:588472/139‑1 (MQ=255)

                                                                         ccACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTa                                                  <  2:223591/139‑1 (MQ=255)

                                                                           aCTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACg                                                <  2:522751/139‑1 (MQ=255)

                                                                             tcctcACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCa                                              <  2:178624/139‑1 (MQ=255)

                                                                                       ggCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTa                                                  >  2:377115/1‑125 (MQ=255)

                                                                                       ggCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTa                                                  <  1:377115/125‑1 (MQ=255)

                                                                                       ggCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTa                                                  >  2:421428/1‑125 (MQ=255)

                                                                                       ggCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTa                                                  <  1:421428/125‑1 (MQ=255)

                                                                                       ggCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGt                                    >  2:7016/1‑139 (MQ=255)

                                                                                                    cTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAAc                                                          <  1:115313/104‑1 (MQ=255)

                                                                                                    cTGTGGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAAc                                                          >  2:115313/1‑104 (MQ=255)

                                                                                                     tgtgGGTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGc                      <  1:747745/139‑1 (MQ=255)

                                                                                                        gggTATACGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAgg                   <  2:681331/139‑1 (MQ=255)

                                                                                                              aCGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGc             <  1:196253/139‑1 (MQ=255)

                                                                                                              aCGATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGc             <  2:792737/139‑1 (MQ=255)

                                                                                                                gATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTg           <  2:55520/139‑1 (MQ=255)

                                                                                                                gATGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTg           <  2:299097/139‑1 (MQ=255)

                                                                                                                 aTGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTa                                                  >  1:427108/1‑99 (MQ=255)

                                                                                                                 aTGCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTa                                                  <  2:427108/99‑1 (MQ=255)

                                                                                                                   gCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAgg                                                                                                 >  2:572291/1‑50 (MQ=255)

                                                                                                                   gCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAgg                                                                                                 <  1:572291/50‑1 (MQ=255)

                                                                                                                   gCAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGata        >  1:160871/1‑139 (MQ=255)

                                                                                                                    cAGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGataa       >  1:44303/1‑139 (MQ=255)

                                                                                                                     aGCGATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGataat      >  2:467597/1‑139 (MQ=255)

                                                                                                                        gATTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATaaa   <  2:112920/139‑1 (MQ=255)

                                                                                                                         aTTCGCCTGCTGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAAc  >  2:44950/1‑139 (MQ=255)

AGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGATCAAGGTCTTCGACTTTAGTACGATCCGAGAAACCGAACTTACGCAATACATCAGTACGGAACATCCCGCAGGCACCGCTGATAATAAAC  >  NC_000913/383128‑383387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A