Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,733,703	G→A	1539 (TAG→TAA)	lhr →	putative ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,733,703	0	G	A	100.0%	154.6 / NA	48	1539 (TAG→TAA)	lhr	putative ATP‑dependent helicase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/26); total (22/26)

TGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGATACATTTCG  >  NC_000913/1733573‑1733838

tGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGa                                                                                                                                 >  2:668782/1‑139 (MQ=255)

tGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGa                                                                                                                                 >  1:60163/1‑139 (MQ=255)

 gCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGAc                                                                                                                                <  1:1218429/139‑1 (MQ=255)

  cGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACt                                                                                                                               >  2:617100/1‑139 (MQ=255)

              cGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCg                                                                                                                   >  1:695663/1‑139 (MQ=255)

                  tGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCg                                                                                                               <  1:617100/139‑1 (MQ=255)

                    cgcTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTc                                                                                                                >  2:319686/1‑136 (MQ=255)

                    cgcTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTc                                                                                                                <  1:319686/136‑1 (MQ=255)

                    cgcTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCa                                                                                                             <  2:507653/139‑1 (MQ=255)

                    cgcTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCa                                                                                                             <  1:1160281/139‑1 (MQ=255)

                              tAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTa                                                                                                   <  1:668782/139‑1 (MQ=255)

                                 cAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTg                                                                                                >  2:720251/1‑139 (MQ=255)

                                      gTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAgg                                                                                            >  2:129949/1‑138 (MQ=255)

                                      gTGAATGATCTACCGGTCCGGAAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAgg                                                                                            <  1:129949/138‑1 (MQ=255)

                                         aaTGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAgg                                                                                            <  1:326260/135‑1 (MQ=255)

                                         aaTGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAgg                                                                                            >  2:326260/1‑135 (MQ=255)

                                          aTGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGa                                                                                       >  2:111069/1‑139 (MQ=255)

                                           tGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGat                                                                                      <  2:695663/139‑1 (MQ=255)

                                              tCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGattat                                                                                   >  1:787671/1‑139 (MQ=255)

                                                   cGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCcgc                                                                              >  2:756344/1‑139 (MQ=255)

                                                     gTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCTGTTCGCATCCGTCAGTATTGCAGGACGGAGTATTCCGCGt                                                                            >  2:705054/1‑139 (MQ=255)

                                                        cGGCAAACTCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAg                                                                                                     <  1:825302/111‑1 (MQ=255)

                                                        cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAg                                                                                                     >  2:825302/1‑111 (MQ=255)

                                                        cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCg                                                                         >  2:17002/1‑139 (MQ=255)

                                                        cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCg                                                                         >  2:1160689/1‑139 (MQ=255)

                                                        cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCg                                                                         >  1:820220/1‑139 (MQ=255)

                                                         ggCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCgg                                                                        <  1:756344/139‑1 (MQ=255)

                                                          gCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGc                                                                       >  2:470379/1‑139 (MQ=255)

                                                              aCGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCtt                                                                   <  1:470379/139‑1 (MQ=255)

                                                                gCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAgg                                                                                            >  2:1052604/1‑112 (MQ=255)

                                                                gCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAgg                                                                                            <  1:1052604/112‑1 (MQ=255)

                                                                   gATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGAc                                                              <  1:720251/139‑1 (MQ=255)

                                                                                   cGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAg                                              <  2:478432/139‑1 (MQ=255)

                                                                                        ggCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATaaagaaag                                                                                                                                   >  2:169867/1‑49 (MQ=255)

                                                                                        ggCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATaaagaaag                                                                                                                                   <  1:169867/49‑1 (MQ=255)

                                                                                           gggATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTg                                      <  1:187751/139‑1 (MQ=255)

                                                                                             gATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCATCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGAt                                    >  2:586172/1‑139 (MQ=255)

                                                                                               ttttCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCa                                  <  1:111069/139‑1 (MQ=255)

                                                                                                tttCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCGGACTTGTATTTAGCGGCAGTTTCTTCGATCAg                                 <  2:624500/139‑1 (MQ=255)

                                                                                                      gTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGctgctg                           <  2:820220/139‑1 (MQ=255)

                                                                                                              caAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGc                   <  2:852118/139‑1 (MQ=255)

                                                                                                                aGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTACAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCa                 <  2:1214446/139‑1 (MQ=255)

                                                                                                                    cTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCt             >  1:296894/1‑139 (MQ=255)

                                                                                                                    cTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCt             >  1:1202349/1‑139 (MQ=255)

                                                                                                                     tGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTg            <  1:705054/139‑1 (MQ=255)

                                                                                                                      ggATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGa           <  1:17002/139‑1 (MQ=255)

                                                                                                                            gggATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCa                                                                 <  2:226674/79‑1 (MQ=255)

                                                                                                                            gggATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCa                                                                 >  1:226674/1‑79 (MQ=255)

                                                                                                                            gggATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGATACAtt     <  2:1202349/139‑1 (MQ=255)

                                                                                                                               aTAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGATACATTTCg  <  2:296894/139‑1 (MQ=255)

TGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGATACATTTCG  >  NC_000913/1733573‑1733838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A