Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I1 R1	770	68.5	3470224	91.7%	3182195	103.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,733,703	G→A	1539 (TAG→TAA)	lhr →	putative ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,733,703	0	G	A	100.0%	167.7 / NA	52	1539 (TAG→TAA)	lhr	putative ATP‑dependent helicase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/28); total (24/28)

GCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGA  >  NC_000913/1733574‑1733829

gCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGAc                                                                                                                       <  2:1186425/139‑1 (MQ=255)

gCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGAc                                                                                                                       >  1:644504/1‑139 (MQ=255)

                      tttACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATcc                                                                                                 <  2:5749/139‑1 (MQ=255)

                           gCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAg                                                                                            <  1:1455528/139‑1 (MQ=255)

                                  gAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCa                                                                                     <  1:769371/139‑1 (MQ=255)

                                    aGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAgg                                                                                   >  2:1387205/1‑139 (MQ=255)

                                         aTGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGCTTGGGGATAAAGAAAGGACTGACGGATGCCCTTTCGCATCCGTCAGTATTGCAGGACGGa                                                                              >  1:1038851/1‑139 (MQ=255)

                                             tCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGattat                                                                          >  1:950809/1‑139 (MQ=255)

                                               tACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTc                                                                        >  2:1168415/1‑139 (MQ=255)

                                                aCCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGaaa                                                                                                                           <  1:144499/87‑1 (MQ=255)

                                                aCCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGaaa                                                                                                                           >  2:144499/1‑87 (MQ=255)

                                                  cGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACg                                                                                                                  >  2:87122/1‑94 (MQ=255)

                                                  cGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACg                                                                                                                  <  1:87122/94‑1 (MQ=255)

                                                   ggTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCcgcg                                                                    <  2:57262/139‑1 (MQ=255)

                                                     tCCGGCAAACGCCGATGTTTATGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTCGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCATGACGGATTATTCCGCGTc                                                                  <  2:1008744/139‑1 (MQ=255)

                                                       cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCg                                                                >  2:824574/1‑139 (MQ=255)

                                                       cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCg                                                                >  1:1366044/1‑139 (MQ=255)

                                                       cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCg                                                                >  1:1307108/1‑139 (MQ=255)

                                                       cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCg                                                                >  2:1474075/1‑139 (MQ=255)

                                                        ggCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCgg                                                               <  2:260100/139‑1 (MQ=255)

                                                        ggCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCgg                                                               <  2:644504/139‑1 (MQ=255)

                                                             aCGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCtt                                                          <  2:1307108/139‑1 (MQ=255)

                                                               gCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCa                                                        >  2:933253/1‑139 (MQ=255)

                                                                  gATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGAc                                                     <  2:1366044/139‑1 (MQ=255)

                                                                              gctgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACt                                                                                                                      >  2:227736/1‑62 (MQ=255)

                                                                              gctgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACt                                                                                                                      <  1:227736/62‑1 (MQ=255)

                                                                               ctgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCcgcg                                                                    >  2:1685067/1‑111 (MQ=255)

                                                                               ctgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCcgcg                                                                    <  1:1685067/111‑1 (MQ=255)

                                                                               ctgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCgg                                        <  2:950809/139‑1 (MQ=255)

                                                                                tgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGc                                       >  2:1105913/1‑139 (MQ=255)

                                                                                  cGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAg                                     <  1:1387205/139‑1 (MQ=255)

                                                                                  cGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAg                                     <  1:1474075/139‑1 (MQ=255)

                                                                                            gATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAg                                     <  2:1251708/129‑1 (MQ=255)

                                                                                            gATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAg                                     >  1:1251708/1‑129 (MQ=255)

                                                                                                        cGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGa                            >  2:793690/1‑126 (MQ=255)

                                                                                                        cGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGa                            <  1:793690/126‑1 (MQ=255)

                                                                                                             caAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGc          <  1:933253/139‑1 (MQ=255)

                                                                                                                 ggCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACg      <  1:1105913/139‑1 (MQ=255)

                                                                                                                 ggCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACg      <  1:824574/139‑1 (MQ=255)

                                                                                                                   cTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCt    >  2:1401619/1‑139 (MQ=255)

                                                                                                                     ggATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCa                                                                                                    >  1:571975/1‑41 (MQ=255)

                                                                                                                     ggATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCa                                                                                                    <  2:571975/41‑1 (MQ=255)

                                                                                                                           gggATAAAGAAAGGACTGACGGATGCCCGTTCGCa                                                                                                    >  1:1416834/1‑35 (MQ=255)

                                                                                                                           gggATAAAGAAAGGACTGACGGATGCCCGTTCGCa                                                                                                    <  2:1416834/35‑1 (MQ=255)

                                                                                                                           gggATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAg                                                                                            <  1:606049/43‑1 (MQ=255)

                                                                                                                           gggATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAg                                                                                            >  2:606049/1‑43 (MQ=255)

                                                                                                                           gggATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCa                                                        >  2:185170/1‑79 (MQ=255)

                                                                                                                           gggATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCa                                                        <  1:185170/79‑1 (MQ=255)

                                                                                                                           gggATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGttt                                  >  1:1467638/1‑101 (MQ=255)

                                                                                                                           gggATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGttt                                  <  2:1467638/101‑1 (MQ=255)

                                                                                                                           gggATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGa  <  1:658660/133‑1 (MQ=255)

                                                                                                                           gggATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGa  >  2:658660/1‑133 (MQ=255)

GCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGA  >  NC_000913/1733574‑1733829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A