Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I2 R1
|
766 |
55.6 |
2631458 |
94.7% |
2491990 |
109.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,299,999 |
G→A |
A12T (GCC→ACC) |
yhbQ → |
GIY‑YIG nuclease superfamily protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,299,999 | 0 | G | A | 98.2%
| 173.6
/ NA
| 57 | A12T (GCC→ACC) | yhbQ | GIY‑YIG nuclease superfamily protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (30/26); total (31/26) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
ACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGA > NC_000913/3299869‑3300132
|
acaTGTTGTTTTGCCAGCCTACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGAGCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCAGCGACAAt < 2:860318/139‑1 (MQ=255)
ttgttTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt > 2:1285496/1‑139 (MQ=255)
ttgttTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt < 2:906214/139‑1 (MQ=255)
gccagccaACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAAt > 1:915693/1‑127 (MQ=255)
gccagccaACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAAt < 2:915693/127‑1 (MQ=255)
ccagccaACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCg > 2:817798/1‑139 (MQ=255)
tGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAc > 2:3067/1‑139 (MQ=255)
tgtTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAcgccg < 1:817798/139‑1 (MQ=255)
tttCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCgg > 2:1158683/1‑113 (MQ=255)
tttCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCgg < 1:1158683/113‑1 (MQ=255)
tttCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCt < 1:1285496/139‑1 (MQ=255)
tttCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCt > 2:914088/1‑139 (MQ=255)
tCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTAt < 1:1066221/139‑1 (MQ=255)
ttGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCATACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATcagc > 2:1054530/1‑139 (MQ=255)
ttCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCAcc > 1:1228117/1‑139 (MQ=255)
ataCTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCa < 2:1228117/139‑1 (MQ=255)
ctttgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATcacc < 1:226653/93‑1 (MQ=255)
ctttgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATcacc > 2:226653/4‑96 (MQ=255)
tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCggg > 2:478977/1‑87 (MQ=255)
tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCggg < 1:478977/87‑1 (MQ=255)
tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCggg < 1:151246/87‑1 (MQ=255)
tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCggg > 2:151246/1‑87 (MQ=255)
tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAcgc > 1:646496/1‑108 (MQ=255)
tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAcgc < 2:646496/108‑1 (MQ=255)
tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgggg > 2:333754/1‑139 (MQ=255)
tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgggg > 1:108510/1‑139 (MQ=255)
ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAATCGGCAAAGGGGCg > 2:847989/1‑139 (MQ=255)
ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAAt > 2:742322/1‑70 (MQ=255)
ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAAt < 1:742322/70‑1 (MQ=255)
ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATcag > 2:1217925/1‑115 (MQ=255)
ggCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATcag < 1:1217925/115‑1 (MQ=255)
cTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAcg > 1:97053/1‑102 (MQ=255)
cTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAcg < 2:97053/102‑1 (MQ=255)
cTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt > 2:1184646/1‑67 (MQ=255)
cTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt < 1:1184646/67‑1 (MQ=255)
gCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATcac < 2:565018/74‑1 (MQ=255)
gCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATcac > 1:565018/1‑74 (MQ=255)
gCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGa < 1:914088/139‑1 (MQ=255)
cGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCggg < 2:314011/68‑1 (MQ=255)
cGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCggg > 1:314011/1‑68 (MQ=255)
gACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAcg > 2:643775/1‑87 (MQ=255)
gACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAcg < 1:643775/87‑1 (MQ=255)
cGCAGACGATGACACCCTGGTTTCTTTACTTGATCCATACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAgg < 1:1054530/139‑1 (MQ=255)
gCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGga > 2:157900/1‑139 (MQ=255)
cAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGgag > 2:919200/1‑139 (MQ=255)
gACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTg > 2:792830/1‑124 (MQ=255)
gACGATGACACCCTGGTTTCATTACTTGATCCGTACCACCGACAATAACCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTg < 1:792830/124‑1 (MQ=255)
gACCCCCTTGTTTCTTTACTTGGCCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACg < 2:566094/139‑1 (MQ=255)
acCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTg > 1:1283954/1‑139 (MQ=255)
ccTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTgcg < 2:1046611/115‑1 (MQ=255)
ccTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTgcg > 1:1046611/1‑115 (MQ=255)
ccTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGgt > 1:249928/1‑139 (MQ=255)
cTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCa < 2:1283954/139‑1 (MQ=255)
tGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGt > 2:932837/1‑139 (MQ=255)
gTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGa > 2:719029/1‑139 (MQ=255)
tACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCaaa > 1:870801/1‑76 (MQ=255)
tACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCaaa < 2:870801/76‑1 (MQ=255)
|
ACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGA > NC_000913/3299869‑3300132
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
