Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I2 R1
|
764 |
42.0 |
2018750 |
95.5% |
1927906 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,299,999 |
G→A |
A12T (GCC→ACC) |
yhbQ → |
GIY‑YIG nuclease superfamily protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,299,999 | 0 | G | A | 100.0%
| 95.6
/ NA
| 31 | A12T (GCC→ACC) | yhbQ | GIY‑YIG nuclease superfamily protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/12); total (19/12) |
ACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGG > NC_000913/3299869‑3300129
|
acaTGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAAt < 1:821350/139‑1 (MQ=255)
gccaACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGa > 1:608960/1‑139 (MQ=255)
gATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt > 1:443898/1‑112 (MQ=255)
gATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt < 2:443898/112‑1 (MQ=255)
gATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCggg > 2:600262/1‑122 (MQ=255)
gATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCggg < 1:600262/122‑1 (MQ=255)
ccATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATAcc < 2:686726/108‑1 (MQ=255)
ccATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATAcc > 1:686726/1‑108 (MQ=255)
gTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATAcc > 2:563494/1‑100 (MQ=255)
gTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATAcc < 1:563494/100‑1 (MQ=255)
ttCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGGTTCTTTACTTGATCCGTACCACCGCCAATAAGCTTTATACCGGGATCACCACGGATCTCGAACGCCGCTATCAGCCGCAcc > 1:308105/1‑139 (MQ=255)
ctctTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCa < 1:265984/138‑1 (MQ=255)
ctctTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCa > 2:265984/1‑138 (MQ=255)
tgGGCCTGACCTTTACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATaa > 2:902826/1‑74 (MQ=255)
tgGGCCTGACCTTTACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATaa < 1:902826/74‑1 (MQ=255)
tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACgg > 2:352119/1‑97 (MQ=255)
tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACgg < 1:352119/97‑1 (MQ=255)
tgGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCATAgggg > 1:518198/1‑139 (MQ=255)
aaCATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTgcg > 2:306605/1‑139 (MQ=255)
aaCATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTgcg > 1:362621/1‑139 (MQ=255)
gCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCggg > 1:612774/1‑69 (MQ=255)
gCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCggg < 2:612774/69‑1 (MQ=255)
cGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGaa > 1:624048/1‑139 (MQ=255)
aCGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAg < 1:536651/139‑1 (MQ=255)
ccTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCgg < 2:180640/118‑1 (MQ=255)
ccTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCgg > 1:180640/1‑118 (MQ=255)
ccTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGgt > 2:35191/1‑139 (MQ=255)
tGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCaaa < 1:838077/96‑1 (MQ=255)
tGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCaaa > 2:838077/1‑96 (MQ=255)
tCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTggg > 2:723224/1‑139 (MQ=255)
tCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTggg > 1:127904/1‑139 (MQ=255)
|
ACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTATACTGTGGGCCTGACCTTAACATGCGACGCAGACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGG > NC_000913/3299869‑3300129
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
