Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I1 R1	778	68.7	3228504	93.4%	3015422	109.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,886,810	G→A	322 (TAG→TAA)	yeaX →	putative YeaWX dioxygenase beta subunit, reductase component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,886,810	0	G	A	100.0%	193.2 / NA	60	322 (TAG→TAA)	yeaX	putative YeaWX dioxygenase beta subunit, reductase component
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (34/26); total (34/26)

CCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAA  >  NC_000913/1886674‑1886945

ccTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCAAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAAtt                                                                                                                                       >  1:952373/1‑139 (MQ=255)

 cTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAAttt                                                                                                                                      >  2:632444/1‑139 (MQ=255)

   gCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGc                                                                                                                                    >  1:833682/1‑139 (MQ=255)

   gCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGc                                                                                                                                    >  1:1561232/1‑139 (MQ=255)

               tACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGAtg                                                                                                                        <  1:632444/139‑1 (MQ=255)

                      aGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCt                                                                                                                 >  2:1125893/1‑139 (MQ=255)

                               tGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGcc                                                                                                        <  2:591969/139‑1 (MQ=255)

                                  ccATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGcc                                                                                                                                   <  2:1375820/109‑1 (MQ=255)

                                  ccATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGcc                                                                                                                                   >  1:1375820/1‑109 (MQ=255)

                                             aaTATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCgg                                                                                          >  2:143123/1‑139 (MQ=255)

                                                   ttAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCg                                                                                           >  2:1115108/1‑132 (MQ=255)

                                                   ttAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCg                                                                                           <  1:1115108/132‑1 (MQ=255)

                                                    tAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCtt                                                                                             >  2:511978/1‑129 (MQ=255)

                                                    tAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCtt                                                                                             <  1:511978/129‑1 (MQ=255)

                                                        gATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACt                                                                               <  1:637740/139‑1 (MQ=255)

                                                                  gTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTc                                                                     <  1:1125893/139‑1 (MQ=255)

                                                                    gccagccaGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACt                                                                               <  2:1035356/127‑1 (MQ=255)

                                                                    gccagccaGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACt                                                                               >  1:1035356/1‑127 (MQ=255)

                                                                       agccagcAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCa                                                                <  1:806797/139‑1 (MQ=255)

                                                                         ccagcAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAAt                                                              >  2:772706/1‑139 (MQ=255)

                                                                              aaaGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAgcgcg                                                                                                          <  1:1520539/90‑1 (MQ=255)

                                                                              aaaGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAgcgcg                                                                                                          >  2:1520539/1‑90 (MQ=255)

                                                                                    tGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTaa                                                   <  1:772706/139‑1 (MQ=255)

                                                                                       tGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGa                                                                          >  2:1561654/1‑113 (MQ=255)

                                                                                       tGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGa                                                                          <  1:1561654/113‑1 (MQ=255)

                                                                                          tCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCa                                                                <  1:1342038/120‑1 (MQ=255)

                                                                                          tCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCa                                                                >  2:1342038/1‑120 (MQ=255)

                                                                                            tgttgtTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAgcgcg                                                                                                          >  2:234686/1‑76 (MQ=255)

                                                                                            tgttgtTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAgcgcg                                                                                                          <  1:234686/76‑1 (MQ=255)

                                                                                            tgttgtTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCtt                                                                                             <  1:1289241/89‑1 (MQ=255)

                                                                                            tgttgtTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCtt                                                                                             >  2:1289241/1‑89 (MQ=255)

                                                                                            tgttgtTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTa                                                                                 <  2:204244/101‑1 (MQ=255)

                                                                                            tgttgtTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTa                                                                                 >  1:204244/1‑101 (MQ=255)

                                                                                            tgttgtTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTa                                                                                 >  2:1022396/1‑101 (MQ=255)

                                                                                            tgttgtTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTa                                                                                 <  1:1022396/101‑1 (MQ=255)

                                                                                            tgttgtTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCg                                           >  1:547619/1‑139 (MQ=255)

                                                                                            tgttgtTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCg                                           >  2:1361233/1‑139 (MQ=255)

                                                                                              ttgttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGcc                                         <  2:833682/139‑1 (MQ=255)

                                                                                                gttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCAt                                       >  2:543863/1‑139 (MQ=255)

                                                                                                   cgcgTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGcc                                                                                                                                   >  2:467247/1‑44 (MQ=255)

                                                                                                   cgcgTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGcc                                                                                                                                   <  1:467247/44‑1 (MQ=255)

                                                                                                   cgcgTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAg                                    >  2:257213/1‑139 (MQ=255)

                                                                                                      gTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGc                                                                                                                              <  2:287229/46‑1 (MQ=255)

                                                                                                      gTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGc                                                                                                                              >  1:287229/1‑46 (MQ=255)

                                                                                                        gCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTcac                               >  1:1600632/1‑139 (MQ=255)

                                                                                                        gCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTcac                               >  1:799836/1‑139 (MQ=255)

                                                                                                                 aaaCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGc                      <  1:143123/139‑1 (MQ=255)

                                                                                                                        tGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCa                                                                <  2:247116/90‑1 (MQ=255)

                                                                                                                        tGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCa                                                                >  1:247116/1‑90 (MQ=255)

                                                                                                                        tGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAAt               >  1:898678/1‑139 (MQ=255)

                                                                                                                         ggTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGc                      <  2:197774/131‑1 (MQ=255)

                                                                                                                         ggTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGc                      >  1:197774/1‑131 (MQ=255)

                                                                                                                               ggATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTc                                                                     >  1:411988/1‑78 (MQ=255)

                                                                                                                               ggATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTc                                                                     <  2:411988/78‑1 (MQ=255)

                                                                                                                               ggATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGc                      <  2:1527142/125‑1 (MQ=255)

                                                                                                                               ggATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGc                      >  1:1527142/1‑125 (MQ=255)

                                                                                                                                 aTTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAg                                    >  1:558533/1‑109 (MQ=255)

                                                                                                                                 aTTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAg                                    <  2:558533/109‑1 (MQ=255)

                                                                                                                                     gTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCaaa  >  1:489058/1‑139 (MQ=255)

                                                                                                                                     gTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCaaa  >  2:1256186/1‑139 (MQ=255)

CCTGCGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCGGCAAA  >  NC_000913/1886674‑1886945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A