Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	773	79.9	4112934	92.2%	3792125	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,886,810	G→A	322 (TAG→TAA)	yeaX →	putative YeaWX dioxygenase beta subunit, reductase component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,886,810	0	G	A	100.0%	218.7 / NA	68	322 (TAG→TAA)	yeaX	putative YeaWX dioxygenase beta subunit, reductase component
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (35/33); total (35/33)

GAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCG  >  NC_000913/1886694‑1886940

gAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGgcg                                                                                                              >  1:1829710/1‑139 (MQ=255)

 aaGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGgcgc                                                                                                             <  2:1829710/139‑1 (MQ=255)

     tGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGcc                                                                                                                        >  1:293265/1‑124 (MQ=255)

     tGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGcc                                                                                                                        <  2:293265/124‑1 (MQ=255)

     tGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAg                                                                                                         >  1:931429/1‑139 (MQ=255)

                       tCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTATTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTc                                                                                       >  1:1753496/1‑139 (MQ=255)

                                   cGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGgcgc                                                                                                             <  2:23043/105‑1 (MQ=255)

                                   cGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGgcgc                                                                                                             >  1:23043/1‑105 (MQ=255)

                                   cGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTAc                                                                           >  2:1188423/1‑139 (MQ=255)

                                    gATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACt                                                                          <  1:1188423/139‑1 (MQ=255)

                                     aTGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTg                                                                         >  2:1755020/1‑139 (MQ=255)

                                        aaGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCgg                                                                      >  2:1126346/1‑139 (MQ=255)

                                             cGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGc                                                                                                                         >  1:1383807/1‑83 (MQ=255)

                                             cGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGc                                                                                                                         <  2:1383807/83‑1 (MQ=255)

                                               tGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGcc                                                                                                                              >  2:1280350/1‑76 (MQ=255)

                                               tGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGcc                                                                                                                              <  1:1280350/76‑1 (MQ=255)

                                                gccagccaGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACt                                                                          >  2:484992/1‑127 (MQ=255)

                                                gccagccaGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACt                                                                          <  1:484992/127‑1 (MQ=255)

                                                gccagccaGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCt                                                              <  2:1698477/139‑1 (MQ=255)

                                                  cagccagcAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCgg                                                                                                                      >  2:452732/1‑81 (MQ=255)

                                                  cagccagcAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCgg                                                                                                                      <  1:452732/81‑1 (MQ=255)

                                                    ttaggagcaggaTGTTGTTCGTTTGTCGGCGGGCAAGGGTT‑AACCGCTTGGTTTGGGATTTTTAATTTGCCAAACCGGGTTGTGGGCCTGGGCGCGCCAAGCCGGGCTTGGGAAGTTTTTATTG‑GGATATTCCTGCAAt                                                         <  2:781794/128‑1 (MQ=255)

                                                     ccagcAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCg                                                                                                                             <  1:543112/71‑1 (MQ=255)

                                                     ccagcAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCg                                                                                                                             >  2:543112/1‑71 (MQ=255)

                                                       agcAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTAc                                                                           <  2:1945439/119‑1 (MQ=255)

                                                       agcAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTAc                                                                           >  1:1945439/1‑119 (MQ=255)

                                                         cAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGAtgt                                                                                                                  >  1:1985073/1‑78 (MQ=255)

                                                         cAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGAtgt                                                                                                                  <  2:1985073/78‑1 (MQ=255)

                                                         cAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGAAGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAAt                                                     >  1:1321149/1‑139 (MQ=255)

                                                               aTGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTa                                               >  1:1618116/1‑139 (MQ=255)

                                                                   tGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGt                                                                                               <  2:1781141/87‑1 (MQ=255)

                                                                   tGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGt                                                                                               >  1:1781141/1‑87 (MQ=255)

                                                                     aTCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCtt                                         <  1:1126346/139‑1 (MQ=255)

                                                                      tCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTc                                        >  2:1992995/1‑139 (MQ=255)

                                                                        tgttgtTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGcc                                                                                                   <  1:1900615/78‑1 (MQ=255)

                                                                        tgttgtTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGcc                                                                                                   >  2:1900615/1‑78 (MQ=255)

                                                                        tgttgtTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTc                                                                >  2:1018247/1‑113 (MQ=255)

                                                                        tgttgtTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTc                                                                <  1:1018247/113‑1 (MQ=255)

                                                                          ttgttCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTg                                                                                                           >  1:1614849/1‑68 (MQ=255)

                                                                          ttgttCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTg                                                                                                           <  2:1614849/68‑1 (MQ=255)

                                                                          ttgttCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTc                                                                >  2:1424851/1‑111 (MQ=255)

                                                                          ttgttCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTc                                                                <  1:1424851/111‑1 (MQ=255)

                                                                          ttgttCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGG‑TATTCCTGCAATAAATTGGGTaactggctcttat                                   >  1:781794/1‑128 (MQ=255)

                                                                            gttCGCGTGAGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCAt                                  <  2:1321149/139‑1 (MQ=255)

                                                                               cgcgTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACt                                                                          <  1:445273/96‑1 (MQ=255)

                                                                               cgcgTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACt                                                                          >  2:445273/1‑96 (MQ=255)

                                                                               cgcgTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAg                               <  2:676003/139‑1 (MQ=255)

                                                                                    gCGAAGGGTAAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCg                                                                                      >  1:646504/1‑80 (MQ=255)

                                                                                    gCGAAGGGTAAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCg                                                                                      <  2:646504/80‑1 (MQ=255)

                                                                                    gCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGAt                                                                                                                    >  1:1146046/1‑49 (MQ=255)

                                                                                    gCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGAt                                                                                                                    <  2:1146046/49‑1 (MQ=255)

                                                                                        aGGGT‑AAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACg                      <  2:1618116/139‑1 (MQ=255)

                                                                                              aaaCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGc                 <  2:1753496/139‑1 (MQ=255)

                                                                                               aaCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGcc                                                                                                   <  1:882815/56‑1 (MQ=255)

                                                                                               aaCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGcc                                                                                                   >  2:882815/1‑56 (MQ=255)

                                                                                               aaCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGcc                >  2:1111732/1‑139 (MQ=255)

                                                                                                aCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCCGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGGCAGTCg               <  1:1992995/139‑1 (MQ=255)

                                                                                                     tGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGcc                                                                                                   <  2:837529/50‑1 (MQ=255)

                                                                                                     tGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGcc                                                                                                   >  1:837529/1‑50 (MQ=255)

                                                                                                     tGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGa                                                                     >  1:436183/1‑80 (MQ=255)

                                                                                                     tGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGa                                                                     <  2:436183/80‑1 (MQ=255)

                                                                                                     tGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAAt          >  2:1517550/1‑139 (MQ=255)

                                                                                                      ggTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATc         >  2:1201116/1‑139 (MQ=255)

                                                                                                       gtgtTGGATTTGTAATTTGCCGAAGCCGGATGTGgcgc                                                                                                             >  1:91020/1‑38 (MQ=255)

                                                                                                       gtgtTGGATTTGTAATTTGCCGAAGCCGGATGTGgcgc                                                                                                             <  2:91020/38‑1 (MQ=255)

                                                                                                       gtgtTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCg                                                                       >  1:1668719/1‑76 (MQ=255)

                                                                                                       gtgtTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCg                                                                       <  2:1668719/76‑1 (MQ=255)

                                                                                                             gATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCg  <  1:1755020/139‑1 (MQ=255)

GAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGT‑AAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAGCTCCG  >  NC_000913/1886694‑1886940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A