Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A6 F1 I1 R1 | 750 | 51.0 | 2595496 | 93.6% | 2429384 | 106.4 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,886,810 | G→A | *322* (TAG→TAA) | yeaX → | putative YeaWX dioxygenase beta subunit, reductase component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,886,810 | 0 | G | A | 100.0% | 90.4 / NA | 29 | *322* (TAG→TAA) | yeaX | putative YeaWX dioxygenase beta subunit, reductase component |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/13); total (16/13) | |||||||||||
CGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAG > NC_000913/1886678‑1886935 | cGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGcc > 1:1078374/1‑139 (MQ=255) aCAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAg < 2:1078374/139‑1 (MQ=255) cATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGt > 2:1210262/1‑139 (MQ=255) gggATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCgg > 2:1278621/1‑139 (MQ=255) aTGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCg > 1:304624/1‑126 (MQ=255) aTGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCg < 2:304624/126‑1 (MQ=255) aTGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTg > 2:533567/1‑139 (MQ=255) aaGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAgc > 1:1277757/1‑105 (MQ=255) aaGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAgc < 2:1277757/105‑1 (MQ=255) gccagccaGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCt > 1:920111/1‑139 (MQ=255) ccagcAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAAt < 2:920111/139‑1 (MQ=255) tCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGcc < 1:916484/53‑1 (MQ=255) tCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGGAATTTGcc > 2:916484/1‑53 (MQ=255) ttgttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGcc > 1:1158753/1‑49 (MQ=255) ttgttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGcc < 2:1158753/49‑1 (MQ=255) ttgttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCgg < 2:1220044/57‑1 (MQ=255) ttgttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCgg > 1:1220044/1‑57 (MQ=255) ttgttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGAtg < 1:814249/60‑1 (MQ=255) ttgttCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGAtg > 2:814249/1‑60 (MQ=255) cgcgTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTAc > 1:43457/1‑95 (MQ=255) cgcgTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTAc < 2:43457/95‑1 (MQ=255) cgcgTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAg > 1:314842/1‑139 (MQ=255) cGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCgg > 2:1289456/1‑46 (MQ=255) cGAAGGGTAAACGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCgg < 1:1289456/46‑1 (MQ=255) aaCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGcc > 1:936298/1‑139 (MQ=255) aCGCCTGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCg < 2:314842/139‑1 (MQ=255) tGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAgcgcg < 1:899170/48‑1 (MQ=255) tGGTGTTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAgcgcg > 2:899170/1‑48 (MQ=255) tgtTGGATTTGTAATTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAg < 2:936298/139‑1 (MQ=255) | CGAAACAGCAATACTGGAAGGTGAAGCTGACCATCGGGATCAATATTTTAGCGATGAAGAGCGTGCCAGCCAGCAAAGTATGTTGATCTGTTGTTCGCGTGCGAAGGGTAAACGCCTGGTGTTGGATTTGTAGTTTGCCGAAGCCGGATGTGGCGCTGAGCGCGCCCAGTCCGGCTTCGGAAGATTTTACTGCGGATATTCCTGCAATAAATTGTGTAATGCTTCCGCCATCAGCTCACCACGCCAGCCGGAAATCAG > NC_000913/1886678‑1886935 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |