Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A13 F1 I1 R1 | 778 | 68.7 | 3228504 | 93.4% | 3015422 | 109.7 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,552,094 | G→A | I15I (ATC→ATT) | ypeA ← | GNAT family putative N‑acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,552,094 | 0 | G | A | 100.0% | 213.7 / NA | 65 | I15I (ATC→ATT) | ypeA | GNAT family putative N‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (31/34); total (31/34) | |||||||||||
CCCATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAG > NC_000913/2551960‑2552227 | cccATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGtaatga > 2:352571/1‑139 (MQ=255) ccATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAc < 2:236208/139‑1 (MQ=255) cATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAcc < 1:785283/139‑1 (MQ=255) ccGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGaa > 2:241304/1‑139 (MQ=255) tttACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATa < 2:1432427/139‑1 (MQ=255) tttACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATa < 2:859826/139‑1 (MQ=255) aGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGaa > 1:1163955/1‑128 (MQ=255) aGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGaa < 2:1163955/128‑1 (MQ=255) aGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTAtc > 2:785424/1‑139 (MQ=255) gCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTAt < 2:181027/137‑1 (MQ=255) gCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTAt > 1:181027/1‑137 (MQ=255) cGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAcc < 2:1601653/104‑1 (MQ=255) cGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAcc > 1:1601653/1‑104 (MQ=255) aaaaaCAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATgg < 1:241304/139‑1 (MQ=255) aaacaaacTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTa < 1:352571/139‑1 (MQ=255) aaacTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCg < 2:1342232/139‑1 (MQ=255) acTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAcc < 1:1241447/91‑1 (MQ=255) acTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAcc > 2:1241447/1‑91 (MQ=255) aTGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTg < 2:525284/139‑1 (MQ=255) aTCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTa > 1:1527345/1‑139 (MQ=255) cTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTaaa > 1:1272175/1‑139 (MQ=255) tACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAg < 1:785424/139‑1 (MQ=255) gCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCt > 2:413207/1‑139 (MQ=255) cGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGAt > 2:942963/1‑139 (MQ=255) gATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAACTACGCGTATCTCCATGGTa < 2:1071771/106‑1 (MQ=255) gATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTa > 1:1071771/1‑106 (MQ=255) aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCa < 1:4876/100‑1 (MQ=255) aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCa > 2:4876/1‑100 (MQ=255) aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATgg < 1:711835/103‑1 (MQ=255) aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATgg > 2:711835/1‑103 (MQ=255) aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAg > 1:951015/1‑131 (MQ=255) aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAg < 2:951015/131‑1 (MQ=255) tCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGt > 1:970154/1‑84 (MQ=255) tCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGt < 2:970154/84‑1 (MQ=255) ggATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACgg < 1:194671/139‑1 (MQ=255) gATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGaa > 1:724153/1‑58 (MQ=255) gATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGaa < 2:724153/58‑1 (MQ=255) gTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGaa < 1:942963/139‑1 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTgg > 2:1119100/1‑63 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTgg < 1:1119100/63‑1 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTAtctc < 2:82778/80‑1 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTAtctc > 1:82778/1‑80 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCttttt > 1:1559533/1‑101 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCttttt < 2:1559533/101‑1 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACgg < 2:1162401/133‑1 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACgg > 1:1162401/1‑133 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAAt > 1:1289146/1‑139 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAAt < 2:1181646/139‑1 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAAt > 2:473302/1‑139 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAAt > 1:989764/1‑139 (MQ=255) gcagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCa < 1:684119/72‑1 (MQ=255) gcagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCa > 2:684119/1‑72 (MQ=255) cagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTg > 2:1296374/1‑91 (MQ=255) cagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTg < 1:1296374/91‑1 (MQ=255) tCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAAt < 2:98811/121‑1 (MQ=255) tCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAAt > 1:98811/1‑121 (MQ=255) cGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCg > 2:124552/1‑41 (MQ=255) cGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCg < 1:124552/41‑1 (MQ=255) tCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGc < 1:1556598/88‑1 (MQ=255) tCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGc > 2:1556598/1‑88 (MQ=255) tCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTg > 2:582741/1‑139 (MQ=255) tCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTg > 1:908014/1‑139 (MQ=255) tCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCATATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGaa > 1:1562414/1‑111 (MQ=255) tCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCATATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGaa < 2:1562414/111‑1 (MQ=255) cAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGaaa < 2:908014/139‑1 (MQ=255) aGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAg < 2:1289146/139‑1 (MQ=255) | CCCATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAG > NC_000913/2551960‑2552227 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |