Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A12 F1 I1 R1 | 760 | 58.2 | 3022526 | 90.8% | 2744453 | 102.0 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,552,094 | G→A | I15I (ATC→ATT) | ypeA ← | GNAT family putative N‑acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,552,094 | 0 | G | A | 100.0% | 180.6 / NA | 56 | I15I (ATC→ATT) | ypeA | GNAT family putative N‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/28); total (28/28) | |||||||||||
CCATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAA > NC_000913/2551961‑2552226 | ccATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAc < 2:1416020/139‑1 (MQ=255) cATCTCCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAcc < 2:1091870/139‑1 (MQ=255) cATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAcc > 1:223485/1‑139 (MQ=255) tCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGCCGTCTTGTTTCTTCTTACCCTCGATGTCCATTTCCGGATGGTTCACGGGACCCAGCAGGTGGCAACGCCCCCAAAGGGAAATGCCTTCTTCaaaa > 1:642770/1‑139 (MQ=255) ccGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAAt < 2:858064/139‑1 (MQ=255) gTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTgg < 1:775452/139‑1 (MQ=255) cACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCg > 2:84891/1‑139 (MQ=255) cACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCg > 2:466634/1‑139 (MQ=255) cGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCGCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGaaa < 2:804065/139‑1 (MQ=255) aGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTAtc > 1:751329/1‑139 (MQ=255) cGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTAtct < 1:638085/138‑1 (MQ=255) cGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTAtct > 2:638085/1‑138 (MQ=255) aCCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCa < 1:466634/139‑1 (MQ=255) acTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGtt > 1:897269/1‑139 (MQ=255) ggTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCg < 2:897269/139‑1 (MQ=255) cATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCATATCT‑CCAT‑GGTATTCGTTACCTTATTGCGGGtt > 1:965971/1‑139 (MQ=255) cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGc > 2:1370105/1‑139 (MQ=255) cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGc < 2:500744/139‑1 (MQ=255) cGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGAt < 2:965971/139‑1 (MQ=255) aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGaaa < 1:1340153/72‑1 (MQ=255) aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGaaa > 2:1340153/1‑72 (MQ=255) aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATa < 2:1245730/88‑1 (MQ=255) aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTAt < 1:583061/95‑1 (MQ=255) aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTAt > 2:583061/1‑95 (MQ=255) aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAACCTTCCTGGCGAAATa > 1:1245730/1‑88 (MQ=255) ggATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACgg < 2:223485/139‑1 (MQ=255) gATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGt > 1:240070/1‑139 (MQ=255) ttCCACGGACGCAGTAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAGGTCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGAtt < 2:763377/120‑1 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTa < 2:217840/87‑1 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTa > 1:217840/1‑87 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGAtt > 1:763377/1‑120 (MQ=255) ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAAt > 1:1278697/1‑139 (MQ=255) ccACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGaa < 1:149457/65‑1 (MQ=255) ccACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGaa > 2:149457/1‑65 (MQ=255) aCGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTa > 1:1239642/1‑101 (MQ=255) aCGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTa < 2:1239642/101‑1 (MQ=255) gcagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAatat > 1:399998/1‑139 (MQ=255) cagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGt < 2:1436904/64‑1 (MQ=255) cagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGt > 1:1436904/1‑64 (MQ=255) cagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCATGGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAatat > 1:616877/1‑139 (MQ=255) cagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGAtt > 2:357000/1‑109 (MQ=255) cagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGAtt < 1:357000/109‑1 (MQ=255) tCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACgg > 2:428922/1‑116 (MQ=255) tCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACgg < 1:428922/116‑1 (MQ=255) tCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCg > 2:241179/1‑86 (MQ=255) tCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCg < 1:241179/86‑1 (MQ=255) gCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgcg < 2:223466/54‑1 (MQ=255) gCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgcg > 1:223466/1‑54 (MQ=255) cAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgcg > 1:682931/1‑53 (MQ=255) cAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgcg < 2:682931/53‑1 (MQ=255) aCGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCATGGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATc < 2:616877/139‑1 (MQ=255) aCGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCg < 2:399998/139‑1 (MQ=255) cTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGtt < 1:84891/139‑1 (MQ=255) tCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCa > 1:153199/1‑55 (MQ=255) tCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCa < 2:153199/55‑1 (MQ=255) tCCCAAAGAGTAATGAGCTCTTCGGAATCTTCCTGGCGAAATACGCGTATCT‑CCCT‑GGTATTTGTTACCTTTTTCCGGGTTAAAAGGATGATTATGGCTTGAAAGGTCGAATTAGCCAATATCAGCCGAAAAtgcgtag > 2:1139718/1‑133 (MQ=255) aaGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGaaaaa > 1:391980/1‑139 (MQ=255) | CCATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCT‑CCAT‑GGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAA > NC_000913/2551961‑2552226 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |