Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	765	53.2	2691310	93.4%	2513683	103.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,552,094	G→A	I15I (ATC→ATT)	ypeA ←	GNAT family putative N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,552,094	0	G	A	100.0%	141.4 / NA	45	I15I (ATC→ATT)	ypeA	GNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/22); total (23/22)

CATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAG  >  NC_000913/2551962‑2552227

cATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGAcc                                                                                                                                 <  2:1177049/139‑1 (MQ=255)

   cACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACctct                                                                                                                              >  2:600162/1‑139 (MQ=255)

   cACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACctct                                                                                                                              <  1:770470/139‑1 (MQ=255)

        ttCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGaa                                                                                                                         >  1:715020/1‑139 (MQ=255)

        ttCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGGTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCACAGGGTAATGACctctccaaa                                                                                                                         >  2:1287698/1‑134 (MQ=255)

              ccACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTc                                                                                                                   <  1:600162/139‑1 (MQ=255)

                  gTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTgg                                                                                                               >  2:1219678/1‑139 (MQ=255)

                            aCCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgc                                                                                                     <  1:76647/139‑1 (MQ=255)

                              cTCAGCGACCAAAAACGAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGt                                                                                                   >  2:765506/1‑139 (MQ=255)

                              cTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAAt                                                                                                         <  1:818091/133‑1 (MQ=255)

                              cTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAAt                                                                                                         >  2:818091/1‑133 (MQ=255)

                               tCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTa                                                                                                  <  1:1219678/139‑1 (MQ=255)

                                     aCCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCa                                                                                            >  1:417510/1‑139 (MQ=255)

                                       cAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATa                                                                                                        >  1:31553/1‑125 (MQ=255)

                                       cAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATa                                                                                                        <  2:31553/125‑1 (MQ=255)

                                          aaacaaacTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTa                                                                                       <  2:417510/139‑1 (MQ=255)

                                                                aTCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgcg                                                                                                    >  1:72996/1‑104 (MQ=255)

                                                                aTCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcgcg                                                                                                    <  2:72996/104‑1 (MQ=255)

                                                                aTCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTa                                                                 >  1:343267/1‑139 (MQ=255)

                                                                      cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCg                                                                                                             >  1:952922/1‑89 (MQ=255)

                                                                      cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTccg                                                                                            <  2:874997/106‑2 (MQ=255)

                                                                      cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCa                                                                                            >  1:874997/1‑106 (MQ=255)

                                                                      cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGc                                                           >  1:230996/1‑139 (MQ=255)

                                                                      cGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCCCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCg                                                                                                             <  2:952922/89‑1 (MQ=255)

                                                                            aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATa                                                                                                        <  1:1286813/88‑1 (MQ=255)

                                                                            aTGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATa                                                                                                        >  2:1286813/1‑88 (MQ=255)

                                                                                       cGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACg                                          >  2:118713/1‑139 (MQ=255)

                                                                                         gATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAg                                                             >  2:973457/1‑118 (MQ=255)

                                                                                         gATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAg                                                             <  1:973457/118‑1 (MQ=255)

                                                                                             gTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGaa                                    <  2:143226/139‑1 (MQ=255)

                                                                                             gTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGaa                                    <  1:1176339/139‑1 (MQ=255)

                                                                                              ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTg                                                                        >  1:858432/1‑102 (MQ=255)

                                                                                              ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTg                                                                        <  2:858432/102‑1 (MQ=255)

                                                                                              ttCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAAt                                   >  2:1231407/1‑139 (MQ=255)

                                                                                                    ggACGCAGCAAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCa                             >  2:32216/1‑139 (MQ=255)

                                                                                                        gcagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAatat                         <  2:230996/139‑1 (MQ=255)

                                                                                                         cagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGaa                                    >  2:718243/1‑127 (MQ=255)

                                                                                                         cagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGaa                                    <  1:718243/127‑1 (MQ=255)

                                                                                                         cagcaAGTCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATc                        >  1:782988/1‑139 (MQ=255)

                                                                                                                tCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcg                                                                                                      >  2:1344781/1‑54 (MQ=255)

                                                                                                                tCGCAACGCTCCCAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATAcg                                                                                                      <  1:1344781/54‑1 (MQ=255)

                                                                                                                          cccAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATgg                                                                                         >  2:856496/1‑57 (MQ=255)

                                                                                                                          cccAAAGGGTAATGACCTCTACGAAATCTTCCTGGCGAAATACGCGTATCTCCATgg                                                                                         <  1:856496/57‑1 (MQ=255)

                                                                                                                           ccAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGaa                                                                                                           >  1:214753/1‑38 (MQ=38)

                                                                                                                           ccAAAGGGTAATGACCTCTTCGAAATCTTCCTGGCGaa                                                                                                           <  2:214753/38‑1 (MQ=38)

                                                                                                                               aGGGTAATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAg  <  2:715020/139‑1 (MQ=255)

CATCACCGTTCCGACCACGTCACCGTTTACCTCAGCGACCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCACGGACGCAGCAAGTCGCAACGCTCCCAAAGGGTGATGACCTCTTCGAAATCTTCCTGGCGAAATACGCGTATCTCCATGGTATTCGTTACCTTTTTGCGGGTTAAAAGGCTGATTATGGCGTGAACGGTCGAATTAGCCAATATCTGACGAAAATCGGTTGAAAAAG  >  NC_000913/2551962‑2552227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A