Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I1 R1	770	68.5	3470224	91.7%	3182195	103.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,053,516	(G)_6→7	coding (2663/2745 nt)	torS ←	hybrid sensory histidine kinase in two‑component regulatory system with TorR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,053,510	1	.	G	91.9%	205.7 / 9.0	62	coding (2669/2745 nt)	torS	hybrid sensory histidine kinase in two‑component regulatory system with TorR
Reads supporting (aligned to +/- strand): ref base . (3/2); new base G (31/26); total (34/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

CCGTTAAGTTGTATACCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑GGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGG  >  NC_000913/1053375‑1053642

ccGTTAAGTTGTATACCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑ggg                                                                                                                                    >  2:79471/1‑139 (MQ=255)

    tAAGTTGTATACCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑ggggggg                                                                                                                                <  1:555939/139‑2 (MQ=255)

             taCCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATg                                                                                                                       <  1:287181/139‑1 (MQ=255)

                   aTGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGgc                                                                                                                     >  2:1549270/1‑135 (MQ=255)

                   aTGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGgc                                                                                                                     <  1:1549270/135‑1 (MQ=255)

                     gCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGgctgctgc                                                                                                               >  2:313793/1‑139 (MQ=255)

                     gCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGgctgc                                                                                                                  <  1:742974/136‑1 (MQ=255)

                     gCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGgctgc                                                                                                                  >  2:742974/1‑136 (MQ=255)

                            ttCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGtt                                                                                                        <  1:313793/139‑1 (MQ=255)

                                    gttCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCAt                                                                                                >  2:246520/1‑139 (MQ=255)

                                      tCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAg                                                                                              >  2:1485851/1‑139 (MQ=255)

                                      tCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAg                                                                                              >  2:138353/1‑139 (MQ=255)

                                         tAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑GGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAgctgg                                                                                          <  1:571205/139‑1 (MQ=255)

                                               gCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCtggctggc                                                                                     >  2:1334679/1‑139 (MQ=255)

                                                      ttGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGc                                                                              <  2:45525/139‑1 (MQ=255)

                                                      ttGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGc                                                                              <  1:1204021/139‑1 (MQ=255)

                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTgcg                                                                                                   >  2:274060/1‑115 (MQ=255)

                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTgcg                                                                                                   <  1:274060/115‑1 (MQ=255)

                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAgctggc                                                                                         <  2:276030/125‑1 (MQ=255)

                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAgctggc                                                                                         >  1:276030/1‑125 (MQ=255)

                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCAtt                                                                           >  2:314607/1‑139 (MQ=255)

                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑gg                                                                                                                                     <  1:1613169/81‑1 (MQ=255)

                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑gg                                                                                                                                     >  2:1613169/1‑81 (MQ=255)

                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑g                                                                                                                                      <  1:274852/80‑1 (MQ=255)

                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑g                                                                                                                                      >  2:274852/1‑80 (MQ=255)

                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑GGGGGGCA‑GATAATGgctgctgct                                                                                                              >  1:1546291/1‑103 (MQ=255)

                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑GGGGGGCA‑GATAATGgctgctgct                                                                                                              <  2:1546291/103‑1 (MQ=255)

                                                              aaTCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAg                                                                      >  2:1558094/1‑139 (MQ=255)

                                                              aaTCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAg                                                                      <  1:1042646/139‑1 (MQ=255)

                                                                     ttCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGa                                                                                   >  2:1420863/1‑119 (MQ=255)

                                                                     ttCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGa                                                                                   <  1:1420863/119‑1 (MQ=255)

                                                                                  tATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCaa                                                            >  2:1293570/1‑129 (MQ=255)

                                                                                  tATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCaa                                                            <  1:1293570/129‑1 (MQ=255)

                                                                                    tGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAAc                                                           <  1:397803/128‑1 (MQ=255)

                                                                                    tGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAAc                                                           >  2:397803/1‑128 (MQ=255)

                                                                                    tGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑GGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCt                                               >  2:1489908/1‑139 (MQ=255)

                                                                                     gCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCt                                               >  2:1296279/1‑139 (MQ=255)

                                                                                         ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAg                                                                                              >  2:913346/1‑88 (MQ=255)

                                                                                         ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAg                                                                                              <  1:913346/88‑1 (MQ=255)

                                                                                         ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAg                                                                      <  2:639531/112‑1 (MQ=255)

                                                                                         ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAg                                                                      >  1:639531/1‑112 (MQ=255)

                                                                                         ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑ggggggg                                                                                                                                <  2:1115015/54‑2 (MQ=255)

                                                                                         ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑ggggggg                                                                                                                                >  1:1115015/1‑53 (MQ=255)

                                                                                         ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGAGGAAGGGGGGGCA‑GGTAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATg                                           <  1:1296780/139‑1 (MQ=255)

                                                                                         ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGAGGAAGGGGGGGCA‑GGTAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATg                                           >  2:1296780/1‑139 (MQ=255)

                                                                                            tGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑g                                                                                                                                      >  1:501400/1‑45 (MQ=255)

                                                                                            tGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑g                                                                                                                                      <  2:501400/45‑1 (MQ=255)

                                                                                              cTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCgg                                      <  1:138353/139‑1 (MQ=255)

                                                                                              cTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCgg                                      <  2:491476/139‑1 (MQ=255)

                                                                                                 ccAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCAc                                   >  1:1631114/1‑139 (MQ=255)

                                                                                                 ccAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCAc                                   >  1:1251833/1‑139 (MQ=255)

                                                                                                     agCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCaa                                                            <  2:1254595/110‑1 (MQ=255)

                                                                                                     agCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCaa                                                            >  1:1254595/1‑110 (MQ=255)

                                                                                                         gCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGcgcg                                       >  2:138313/1‑127 (MQ=255)

                                                                                                         gCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGcgcg                                       <  1:138313/127‑1 (MQ=255)

                                                                                                          cAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTAttt                          >  1:382204/1‑139 (MQ=255)

                                                                                                          cAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTAttt                          >  1:1376148/1‑139 (MQ=255)

                                                                                                          cAACACTGCGTGTAATTTCTTCGTGTGGAA‑GGGGGGGAGGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGGGCAACTGCTTTTTAGCTGATGCGCGGCACGCTtttttt                          >  1:520256/1‑139 (MQ=255)

                                                                                                                 gCGTGTTATTTCTTCCTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCt                   <  2:520256/139‑1 (MQ=255)

                                                                                                                 gCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGc                                     >  1:1100259/1‑121 (MQ=255)

                                                                                                                 gCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGc                                     <  2:1100259/121‑1 (MQ=255)

                                                                                                                 gCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCt                   <  2:382204/139‑1 (MQ=255)

                                                                                                                  cGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCa                                                                       <  2:437199/86‑1 (MQ=255)

                                                                                                                  cGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCa                                                                       >  1:437199/1‑86 (MQ=255)

                                                                                                                   gtgtAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTAt                 >  1:1128794/1‑139 (MQ=255)

                                                                                                                    tgtAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATc                >  1:975438/1‑139 (MQ=255)

                                                                                                                            cttcGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTgg        >  1:1431657/1‑139 (MQ=255)

                                                                                                                              tcGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGtt                                                                                                        >  2:347419/1‑41 (MQ=38)

                                                                                                                              tcGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGtt                                                                                                        <  1:347419/41‑1 (MQ=38)

                                                                                                                                  gtgGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCggg  <  1:79471/139‑1 (MQ=255)

                                                                                                                                   tgGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTcc                                                                         <  1:639644/67‑1 (MQ=255)

                                                                                                                                   tgGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTcc                                                                         >  2:639644/1‑67 (MQ=255)

CCGTTAAGTTGTATACCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑GGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGG  >  NC_000913/1053375‑1053642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A