Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I1 R1
|
770 |
68.5 |
3470224 |
91.7% |
3182195 |
103.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,600,589 |
G→A |
S129S (TCC→TCT) |
dnaC ← |
DNA biosynthesis protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,600,589 | 0 | G | A | 100.0%
| 133.6
/ NA
| 42 | S129S (TCC→TCT) | dnaC | DNA biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/19); total (23/19) |
TCTCATCGATCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTA > NC_000913/4600465‑4600722
|
tctcATCGATCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTag > 2:1661779/1‑139 (MQ=255)
aTCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGAtt > 1:1613426/1‑139 (MQ=255)
aTCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGAtt > 1:1617238/1‑139 (MQ=255)
cAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTgc < 1:772799/139‑1 (MQ=255)
aGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCgg > 1:1220691/1‑139 (MQ=255)
tgttCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTc > 1:1541556/1‑139 (MQ=255)
ccGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGa > 2:1163851/1‑137 (MQ=255)
ccGCTATTCCTGAAGGCATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGACTTACCAGa < 1:1163851/137‑1 (MQ=255)
tCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGa < 2:410747/87‑1 (MQ=255)
tCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGa > 1:410747/1‑87 (MQ=255)
ccTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTgccg > 2:560068/1‑92 (MQ=255)
ccTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTgccg < 1:560068/92‑1 (MQ=255)
ccTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTcc < 2:227647/117‑1 (MQ=255)
ccTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTcc > 1:227647/1‑117 (MQ=255)
ggTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACcgcg < 1:387145/60‑1 (MQ=255)
ggTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACcgcg > 2:387145/1‑60 (MQ=255)
tCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGt < 2:551975/139‑1 (MQ=255)
tCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGt < 1:1661779/139‑1 (MQ=255)
tCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGt > 2:1686624/1‑139 (MQ=255)
cTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCTGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGtt > 1:1025749/1‑139 (MQ=255)
cTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGtt > 2:86857/1‑139 (MQ=255)
cGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCa < 2:1613426/139‑1 (MQ=255)
ccGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATATTGCCGTCaaa > 1:1126367/1‑139 (MQ=255)
gACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGc < 1:1402091/88‑1 (MQ=255)
gACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGc > 2:1402091/1‑88 (MQ=255)
gACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAAct < 2:1220691/139‑1 (MQ=255)
atatCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGa > 1:832462/1‑139 (MQ=255)
gCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGtt < 1:1421916/81‑1 (MQ=255)
gCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGtt > 2:1421916/1‑81 (MQ=255)
gCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACt > 2:230631/1‑139 (MQ=255)
gCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACt > 2:1302273/1‑139 (MQ=255)
ccACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTgg < 2:699621/85‑1 (MQ=255)
ccACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTgg > 1:699621/1‑85 (MQ=255)
aCGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGgc < 1:86857/139‑1 (MQ=255)
ggTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGc > 1:953124/1‑71 (MQ=255)
ggTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGc < 2:953124/71‑1 (MQ=255)
gatgatCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCTGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCt < 2:1025749/139‑1 (MQ=255)
gatCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTg > 1:984469/1‑139 (MQ=255)
aaTACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGc < 2:783284/61‑1 (MQ=255)
aaTACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGc > 1:783284/1‑61 (MQ=255)
aaTACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTa < 2:984469/139‑1 (MQ=255)
tACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGttttt < 1:1709670/57‑1 (MQ=255)
|
TCTCATCGATCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTA > NC_000913/4600465‑4600722
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
