Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,600,589	G→A	S129S (TCC→TCT)	dnaC ←	DNA biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,600,589	0	G	A	100.0%	93.5 / NA	30	S129S (TCC→TCT)	dnaC	DNA biosynthesis protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/11); total (19/11)

AGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTAAC  >  NC_000913/4600479‑4600724

agcagATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGc                                                                                                             >  1:486281/1‑139 (MQ=255)

     aTCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACcgcg                                                                                                                              <  1:1040591/117‑1 (MQ=255)

     aTCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACcgcg                                                                                                                              >  2:1040591/1‑117 (MQ=255)

     aTCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGAtt                                                                                                        >  2:982426/1‑139 (MQ=255)

     aTCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGAtt                                                                                                        >  2:518639/1‑139 (MQ=255)

     aTCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGAtt                                                                                                        >  2:360498/1‑139 (MQ=255)

       cAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTgc                                                                                                      >  1:916321/1‑139 (MQ=255)

                    aTCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATgg                                                                                         >  1:805192/1‑139 (MQ=255)

                      cGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGt                                                                                                                             <  1:363796/101‑1 (MQ=255)

                      cGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGt                                                                                                                             >  2:363796/1‑101 (MQ=255)

                               gttgttCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGt                                                                              <  1:322613/139‑1 (MQ=255)

                                          cTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCTGATTGCCGCCGCCAGATGGTTTTTGCCGGCTCCTGGCTTAc                                                                   >  2:438783/1‑139 (MQ=255)

                                          cTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGa                                                                                            <  1:654587/114‑1 (MQ=255)

                                          cTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGa                                                                                            >  2:654587/1‑114 (MQ=255)

                                          cTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTAc                                                                   >  1:888099/1‑139 (MQ=255)

                                                        ccTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAg                                                     <  2:855818/139‑1 (MQ=255)

                                                             aGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGc                                                >  2:598807/1‑139 (MQ=255)

                                                                    tttCATCGCCGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTg                                         <  1:492484/139‑1 (MQ=255)

                                                                            ccGACATAATATCGGCCACGGTGATGATCAATACAGTTTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCaaa                                 <  1:662278/139‑1 (MQ=255)

                                                                            ccGACATAATATCGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCaaa                                 >  1:333658/1‑139 (MQ=255)

                                                                                        cGGCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATa                     <  2:486281/139‑1 (MQ=255)

                                                                                          gCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACt                   >  1:629506/1‑139 (MQ=255)

                                                                                          gCCACGGTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACt                   >  2:808313/1‑139 (MQ=255)

                                                                                               ggTGATGATCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGgcgc              >  2:710917/1‑139 (MQ=255)

                                                                                                   atgatCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCa                                   >  2:43033/1‑114 (MQ=255)

                                                                                                   atgatCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCa                                   <  1:43033/114‑1 (MQ=255)

                                                                                                   atgatCAATACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCtt          <  2:347953/139‑1 (MQ=255)

                                                                                                          aTACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCtt                            <  1:954087/114‑1 (MQ=255)

                                                                                                          aTACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCtt                            >  2:954087/1‑114 (MQ=255)

                                                                                                           tACAGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTAAc  >  2:305424/1‑139 (MQ=255)

AGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCCGACATAATATCGGCCACGGTGATGATCAATACGGATTTACCGCGTAGCAGCAGCTCGTTGCAGATTGCCGCCGCCAGATGGTTTTTGCCGGTTCCTGGCTTACCAGAAAAGATGAAGCTGGCGATGTTGCCGTCAAACTCTTCGACATACTGGCGCGCTTTGCTTAAC  >  NC_000913/4600479‑4600724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A