Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	765	39.6	1867486	94.4%	1762906	108.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,745,362	T→C	T256A (ACA→GCA)	ydhQ ←	autotransporter adhesin‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,745,362	0	T	C	100.0%	174.8 / NA	48	T256A (ACA→GCA)	ydhQ	autotransporter adhesin‑related protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/24); total (24/24)

CAGCTCTGCGCCATAACCAATAATCATTGAAGCGCCGTCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGTCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATCCGTCCCCAGACGATAAATGGAT  >  NC_000913/1745228‑1745495

cAGCTCTGCGCCATAACCAATAATCATTGAAGCGCCGTCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCa                                                                                                                                   <  2:123905/139‑1 (MQ=255)

     ctGCGCCATAACCAATAATCATTGAAGCGCCGTCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTg                                                                                                                              <  1:809880/139‑1 (MQ=255)

        cgcCATAACCAATAATCATTGAAGCGCCGTCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTAt                                                                                                                           >  1:105576/1‑139 (MQ=255)

                 cAATAATCATTGAAGCGCCGTCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGtt                                                                                                                  <  1:413950/139‑1 (MQ=255)

                      atCATTGAAGCGCCGTCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCa                                                                                                             <  2:68275/139‑1 (MQ=255)

                           tGAAGCGCCGTCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCa                                                                                                             <  2:779769/134‑1 (MQ=255)

                           tGAAGCGCCGTCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCa                                                                                                             >  1:779769/1‑134 (MQ=255)

                                     tCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCATCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCa                                                                                              >  2:634023/1‑139 (MQ=255)

                                       cAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACt                                                                                            >  1:862787/1‑139 (MQ=255)

                                             tGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGcc                                                                                      <  2:105576/139‑1 (MQ=255)

                                               cAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCg                                                                                    >  2:62828/1‑139 (MQ=255)

                                               cAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGAATGCACTTCAGCCCg                                                                                    >  2:477798/1‑139 (MQ=255)

                                                aaCACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGa                                                                                   <  1:477798/139‑1 (MQ=255)

                                                    cTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGaccac                                                                               >  2:240903/1‑139 (MQ=255)

                                                       ccGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGac                                                                                  <  2:328930/133‑1 (MQ=255)

                                                       ccGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGac                                                                                  >  1:328930/1‑133 (MQ=255)

                                                          gTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACcgcg                                                                                                                    <  1:329628/96‑1 (MQ=255)

                                                          gTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACcgcg                                                                                                                    >  2:329628/1‑96 (MQ=255)

                                                                 cAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGa                                                                  <  1:240903/139‑1 (MQ=255)

                                                                        tGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGttt                                                           >  2:453433/1‑139 (MQ=255)

                                                                         gcgcGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGtttt                                                          >  2:279571/1‑139 (MQ=255)

                                                                                         cGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACt                                          >  2:290214/1‑139 (MQ=255)

                                                                                         cGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACt                                          >  2:62262/1‑139 (MQ=255)

                                                                                         cGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACt                                          >  2:98559/1‑139 (MQ=255)

                                                                                         cGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACt                                          >  1:125691/1‑139 (MQ=255)

                                                                                         cGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACt                                          >  1:325479/1‑139 (MQ=255)

                                                                                          gACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTg                                         <  2:325479/139‑1 (MQ=255)

                                                                                           aCAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGCACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGt                                        >  1:49085/1‑139 (MQ=255)

                                                                                                         cgcgCTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCa                          <  1:98559/139‑1 (MQ=255)

                                                                                                          gcgcTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCAt                         <  1:290214/139‑1 (MQ=255)

                                                                                                           cgcTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATc                        <  2:125691/139‑1 (MQ=255)

                                                                                                           cgcTGGTGGGTGACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATc                        <  1:62262/139‑1 (MQ=255)

                                                                                                                 tgggtgACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATCCGTccc                  <  1:453433/139‑1 (MQ=255)

                                                                                                                      gACAACAGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATCCGTCCCCAGAc             >  1:659121/1‑139 (MQ=255)

                                                                                                                       acaacaGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATCCGTCCCCAGACg            <  1:341761/139‑1 (MQ=255)

                                                                                                                          acaGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGc                            <  2:293213/120‑1 (MQ=255)

                                                                                                                          acaGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGc                            >  1:293213/1‑120 (MQ=255)

                                                                                                                           caGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGaa                                            <  1:461421/103‑1 (MQ=255)

                                                                                                                           caGGATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGaa                                            >  2:461421/1‑103 (MQ=255)

                                                                                                                              gATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGaccacc                                                                              <  1:914041/66‑1 (MQ=255)

                                                                                                                              gATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGaccacc                                                                              >  2:914041/1‑66 (MQ=255)

                                                                                                                              gATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCgg                                              >  1:303640/1‑98 (MQ=255)

                                                                                                                              gATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCgg                                              <  2:303640/98‑1 (MQ=255)

                                                                                                                              gATCACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATCCGTCCCCAGACGATAAATg     >  2:431175/1‑139 (MQ=255)

                                                                                                                                 cACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATCCGTCCCCAGACGATAAATGGAt  <  1:62828/139‑1 (MQ=255)

                                                                                                                                 cACTGCCCCACCTTGTATTACCGCGTTTTCCAGCGCACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATCCGTCCCCAGACGATAAATGGAt  <  2:49085/139‑1 (MQ=255)

                                                                                                                                   cTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGAccaccca                                                                            <  1:121160/63‑1 (MQ=255)

                                                                                                                                   cTGCCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGAccaccca                                                                            >  2:121160/1‑63 (MQ=255)

CAGCTCTGCGCCATAACCAATAATCATTGAAGCGCCGTCCAGTAATGCAACACTCCCGGTCAGTTCAACCGGTGCGCGATCTTCCTCTACGACAAAATTTTCGTCCGCGCTGGTGGGTGACAACAGGATCACTGTCCCACCTTGTATTACCGCGTTTTCCAGCGTACCGGCATGCACTTCAGCCCGACCACCCACATTCACGGACAGGTTTTGCGTCTTACCGGAACTGTAGAGCTGAAGGCCATCCGTCCCCAGACGATAAATGGAT  >  NC_000913/1745228‑1745495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A