Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I1 R1	765	39.6	1867486	94.4%	1762906	108.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,931,785	T→C	L302P (CTG→CCG)	purT →	phosphoribosylglycinamide formyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,931,785	0	T	C	100.0%	134.6 / NA	37	L302P (CTG→CCG)	purT	phosphoribosylglycinamide formyltransferase 2
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/19); total (18/19)

GCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCG  >  NC_000913/1931649‑1931916

gCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGc                                                                                                                                   <  2:648098/139‑1 (MQ=255)

                     tttGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGAc                                                                                                              >  2:820203/1‑139 (MQ=255)

                     tttGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGAc                                                                                                              >  1:500341/1‑139 (MQ=255)

                          tgtCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCg                                                                                                         >  1:109287/1‑139 (MQ=255)

                           gtCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCgg                                                                                                        <  1:855366/139‑1 (MQ=255)

                               aGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTgg                                                                                                    >  2:690288/1‑139 (MQ=255)

                                    tttGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGa                                                                                               >  1:187955/1‑139 (MQ=255)

                                    tttGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGa                                                                                               <  2:500341/139‑1 (MQ=255)

                                    tttGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGa                                                                                               <  1:554929/139‑1 (MQ=255)

                                         cTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGt                                                                                          <  2:109287/139‑1 (MQ=255)

                                              gtgATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTa                                                                                     <  2:10861/139‑1 (MQ=255)

                                                    aGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTcc                                                                               >  1:134757/1‑139 (MQ=255)

                                                      gTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGAt                                                                                              <  1:351635/122‑1 (MQ=255)

                                                      gTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGAt                                                                                              >  2:351635/1‑122 (MQ=255)

                                                                 tGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACtt                                                                                                            >  2:394712/1‑97 (MQ=255)

                                                                 tGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACtt                                                                                                            <  1:394712/97‑1 (MQ=255)

                                                                  gAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCg                                                                 <  2:187955/139‑1 (MQ=255)

                                                                                  cacaTGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACt                                                 >  1:530070/1‑139 (MQ=255)

                                                                                  cacaTGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACt                                                 >  1:23961/1‑139 (MQ=255)

                                                                                         tACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGt                                          <  1:761731/139‑1 (MQ=255)

                                                                                          aCCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGc                                                                                                                                   >  2:408683/1‑49 (MQ=255)

                                                                                            cGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAg                                       >  2:483848/1‑139 (MQ=255)

                                                                                               gATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGt                                                                                          <  2:831043/85‑1 (MQ=255)

                                                                                               gATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGt                                                                                          >  1:831043/1‑85 (MQ=255)

                                                                                               gATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGAc                                              <  2:244770/129‑1 (MQ=255)

                                                                                               gATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGAc                                              >  1:244770/1‑129 (MQ=255)

                                                                                                  ggTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATc                                                                                             <  1:370203/79‑1 (MQ=255)

                                                                                                  ggTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATc                                                                                             >  2:370203/1‑79 (MQ=255)

                                                                                                  ggTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGc                                                        >  2:531383/1‑116 (MQ=255)

                                                                                                  ggTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGc                                                        <  1:531383/116‑1 (MQ=255)

                                                                                                  ggTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATAGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTc                                 <  1:820203/139‑1 (MQ=255)

                                                                                                            aTTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATa                       <  2:530070/139‑1 (MQ=255)

                                                                                                                 tcAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAAtgtg                  <  1:483848/139‑1 (MQ=255)

                                                                                                                  cAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAAt                     <  1:294092/135‑1 (MQ=255)

                                                                                                                  cAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAAt                     >  2:294092/1‑135 (MQ=255)

                                                                                                                          ctcAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGcc         >  2:870595/1‑139 (MQ=255)

                                                                                                                                 tttGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGgcg  <  1:690288/139‑1 (MQ=255)

GCACTGGGCGGTTATGGGTTGTTTGGTGTCGAGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCG  >  NC_000913/1931649‑1931916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A