Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F1 I2 R1 780 68.3 3343050 93.5% 3125751 102.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,931,785 T→C L302P (CTG→CCG)  purT → phosphoribosylglycinamide formyltransferase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,931,7850TC100.0% 207.1 / NA 57L302P (CTG→CCG) purTphosphoribosylglycinamide formyltransferase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/28);  total (29/28)

AGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCA  >  NC_000913/1931680‑1931918
                                                                                                         |                                                                                                                                     
aGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATg                                                                                                                                  >  1:355597/1‑111 (MQ=255)
aGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATg                                                                                                                                  <  2:355597/111‑1 (MQ=255)
aGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTgg                                                                                                      >  1:1264463/1‑139 (MQ=255)
aGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTgg                                                                                                      >  2:1588582/1‑139 (MQ=255)
             tggtgATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTAc                                                                                                                               >  1:1559935/1‑101 (MQ=255)
             tggtgATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTAc                                                                                                                               <  2:1559935/101‑1 (MQ=255)
                   tgaGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCgg                                                                                                          >  2:1250485/1‑116 (MQ=255)
                   tgaGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCgg                                                                                                          <  1:1250485/116‑1 (MQ=255)
                   tgaGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGt                                                                                   >  1:181049/1‑139 (MQ=255)
                         gATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCg                                                                                                                   <  1:1517958/101‑1 (MQ=255)
                         gATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCg                                                                                                                   >  2:1517958/1‑101 (MQ=255)
                          aTTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAg                                                                            >  1:278322/1‑139 (MQ=255)
                              tCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTc                                                                        <  2:278322/139‑1 (MQ=255)
                                 gTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGc                                                                     >  1:1596910/1‑139 (MQ=255)
                                         tcCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCt                                                                                                                     <  2:31261/83‑1 (MQ=255)
                                         tcCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCt                                                                                                                     >  1:31261/1‑83 (MQ=255)
                                         tcCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTc                                                             >  1:145270/1‑139 (MQ=255)
                                            ccTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGc                                                          <  2:1596910/139‑1 (MQ=255)
                                                gTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTGGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCcaca                                                      >  1:1196848/1‑139 (MQ=255)
                                                gTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCg                                                                                                                   >  1:498212/1‑78 (MQ=255)
                                                gTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCg                                                                                                                   >  1:553576/1‑78 (MQ=255)
                                                gTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCg                                                                                                                   <  2:498212/78‑1 (MQ=255)
                                                gTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCg                                                                                                                   <  2:553576/78‑1 (MQ=255)
                                                gTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCcaca                                                      >  2:540313/1‑139 (MQ=255)
                                                 tCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGc                                                          <  1:42822/134‑1 (MQ=255)
                                                 tCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGc                                                          >  2:42822/1‑134 (MQ=255)
                                                      aTGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGAc                                                <  1:540313/139‑1 (MQ=255)
                                                      aTGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGAc                                                <  2:1264463/139‑1 (MQ=255)
                                                             cGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATAGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAg                                         <  1:1183775/139‑1 (MQ=255)
                                                               ggATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTc                                                                        >  2:614994/1‑106 (MQ=255)
                                                               ggATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTc                                                                        <  1:614994/106‑1 (MQ=255)
                                                                gATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGc                                                                                                                           <  1:333415/54‑1 (MQ=255)
                                                                gATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGc                                                                                                                           >  2:333415/1‑54 (MQ=255)
                                                                gATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAAt                                      >  2:865985/1‑139 (MQ=255)
                                                                   ggTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTc                                   <  1:373919/139‑1 (MQ=255)
                                                                    gTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCcaca                                                      <  2:307555/119‑1 (MQ=255)
                                                                    gTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCcaca                                                      >  1:307555/1‑119 (MQ=255)
                                                                    gTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTc                                   <  2:1543077/138‑1 (MQ=255)
                                                                    gTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTc                                   >  1:1543077/1‑138 (MQ=255)
                                                                        cGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGtt                              <  1:865985/139‑1 (MQ=255)
                                                                             aTTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATa                         <  2:145270/139‑1 (MQ=255)
                                                                             aTTTCTCAAGATCTCTCAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATa                         <  2:1196848/139‑1 (MQ=255)
                                                                                         ctctcAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGa                                                                                                                 <  2:913429/39‑1 (MQ=255)
                                                                                         ctctcAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGa                                                                                                                 >  1:913429/1‑39 (MQ=255)
                                                                                            tcAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTc                                   <  1:676452/114‑1 (MQ=255)
                                                                                            tcAGAGTTTGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTc                                   >  2:676452/1‑114 (MQ=255)
                                                                                                  tttGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGt                                    <  1:583545/107‑1 (MQ=255)
                                                                                                  tttGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGt                                    >  2:583545/1‑107 (MQ=255)
                                                                                                    tGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTAt                                                               >  2:422066/1‑78 (MQ=255)
                                                                                                    tGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTAt                                                               <  1:422066/78‑1 (MQ=255)
                                                                                                    tGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGt                                            <  2:187432/97‑1 (MQ=255)
                                                                                                    tGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGt                                            >  1:187432/1‑97 (MQ=255)
                                                                                                    tGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCa                  >  2:1463650/1‑123 (MQ=255)
                                                                                                    tGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCa                  <  1:1463650/123‑1 (MQ=255)
                                                                                                    tGCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGACGTAGGCGCa  >  2:254330/1‑139 (MQ=255)
                                                                                                     gCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGc                                                                              <  2:16137/62‑1 (MQ=255)
                                                                                                     gCCCCGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGc                                                                              >  1:16137/1‑62 (MQ=255)
                                                                                                      ccccGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCcaca                                                      >  2:1463525/1‑85 (MQ=255)
                                                                                                      ccccGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCcaca                                                      <  1:1463525/85‑1 (MQ=255)
                                                                                                         |                                                                                                                                     
AGCTATTTGTCTGTGGTGATGAGGTGATTTTCAGTGAGGTCTCCCCTCGTCCACATGATACCGGGATGGTGACGTTAATTTCTCAAGATCTCTCAGAGTTTGCCCTGCATGTACGTGCCTTCCTCGGACTTCCGGTTGGCGGGATCCGTCAGTATGGTCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCA  >  NC_000913/1931680‑1931918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A