Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I1 R1
|
765 |
39.6 |
1867486 |
94.4% |
1762906 |
108.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,610,462 |
C→T |
A350V (GCG→GTG) |
prfC → |
peptide chain release factor RF‑3 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,610,462 | 0 | C | T | 100.0%
| 84.6
/ NA
| 27 | A350V (GCG→GTG) | prfC | peptide chain release factor RF‑3 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/13); total (14/13) |
ACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACT > NC_000913/4610345‑4610590
|
accgcgaccgcgTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTga < 1:367975/139‑1 (MQ=255)
tgtgGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACg < 1:200817/110‑1 (MQ=255)
tgtgGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACg > 2:418783/1‑110 (MQ=255)
tgtgGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACg > 2:200817/1‑110 (MQ=255)
tgtgGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACg < 1:418783/110‑1 (MQ=255)
tgtgGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTc > 1:62838/1‑139 (MQ=255)
aaTATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCa < 2:497113/139‑1 (MQ=255)
tGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGcac < 1:185016/130‑1 (MQ=255)
tGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGcac > 2:185016/1‑130 (MQ=255)
aaaaGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCAcc > 1:356201/1‑139 (MQ=255)
tGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa < 2:356201/139‑1 (MQ=255)
cgcCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGaca > 1:468955/1‑139 (MQ=255)
gcgcACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTAcc < 2:468955/139‑1 (MQ=255)
gCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCg > 2:607757/1‑79 (MQ=255)
gCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCg < 1:607757/79‑1 (MQ=255)
gCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTg < 2:330076/139‑1 (MQ=255)
cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa < 2:312516/121‑1 (MQ=255)
cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGa > 1:312516/1‑121 (MQ=255)
cGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTga > 2:394302/1‑139 (MQ=255)
aaGATGTGGTGATCTCCGACGCGCTGACCTTTATGGTGGGTGACAGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAgat > 2:658646/1‑139 (MQ=255)
cgcTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTcc < 1:443213/139‑1 (MQ=255)
cTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCt > 2:361442/1‑100 (MQ=255)
cTGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCt < 1:361442/100‑1 (MQ=255)
tGACCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGaa > 1:387899/1‑139 (MQ=255)
aCCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATc > 2:80043/1‑43 (MQ=255)
aCCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATc < 1:80043/43‑1 (MQ=255)
aCCTTTATGGTGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACt > 1:564776/1‑139 (MQ=255)
|
ACCGCGACCGCGTGGCGTTTATGCGTGTGGTGTCCGGTAAATATGAAAAAGGCATGAAACTGCGCCAGGTGCGCACTGCGAAAGATGTGGTGATCTCCGACGCGCTGACCTTTATGGCGGGTGACCGTTCGCACGTTGAAGAAGCGTATCCGGGCGATATCCTCGGCCTGCACAACCACGGCACCATTCAGATCGGCGACACCTTTACCCAGGGTGAGATGATGAAGTTCACCGGTATTCCGAACT > NC_000913/4610345‑4610590
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
