Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	759	65.2	3386260	90.7%	3071337	101.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	480,334	C→T	125 (TAG→TAA)	tomB ←	Hha toxicity attenuator, conjugation‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	480,334	0	C	T	100.0%	176.3 / NA	55	125 (TAG→TAA)	tomB	Hha toxicity attenuator, conjugation‑related protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (30/25); total (30/25)

TCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTG  >  NC_000913/480199‑480463

tCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTaa                                                                                                                                <  2:1323954/139‑1 (MQ=255)

       aTTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATa                                                                                                                         <  2:1383474/139‑1 (MQ=255)

          cGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGAt                                                                                                                          >  2:552521/1‑135 (MQ=255)

          cGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGAt                                                                                                                          <  1:552521/135‑1 (MQ=255)

          cGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAAc                                                                                                                      >  1:746603/1‑139 (MQ=255)

                                    tCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTTATAATTTTAAc                                                                                                                               <  2:981903/104‑1 (MQ=255)

                                    tCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAAc                                                                                                                               >  1:981903/1‑104 (MQ=255)

                                    tCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTg                                                                                            >  2:825814/1‑139 (MQ=255)

                                         cGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACaa                                                                                        >  2:1393334/1‑138 (MQ=255)

                                         cGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACaa                                                                                        <  1:1393334/138‑1 (MQ=255)

                                            gtCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTtc                                                                                                     <  2:456815/122‑1 (MQ=255)

                                            gtCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTtc                                                                                                     >  1:456815/1‑122 (MQ=255)

                                            gtCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACa                                                                                         >  1:16876/1‑134 (MQ=255)

                                            gtCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACa                                                                                         <  2:16876/134‑1 (MQ=255)

                                            gtCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAaaca                                                                                    >  1:134611/1‑139 (MQ=255)

                                             tCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCg                                                                                                                   <  2:490602/107‑1 (MQ=255)

                                             tCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCg                                                                                                                   >  1:490602/1‑107 (MQ=255)

                                                  tGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACATATGGTTGTAATAATTTTAACAAGATAAACTAGCAGGATTCTCTTTCGTAGCATTGACAAAACAACGGaa                                                                              >  2:110108/1‑139 (MQ=255)

                                                        gCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCg                                                                        <  2:134611/139‑1 (MQ=255)

                                                              acgTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTAcc                                                                  >  1:1529361/1‑139 (MQ=255)

                                                                        aTTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGc                                                        >  1:1621814/1‑139 (MQ=255)

                                                                        aTTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGc                                                        <  1:825814/139‑1 (MQ=255)

                                                                         ttAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGcc                                                       >  2:711115/1‑139 (MQ=255)

                                                                                  cGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGaa                                                                              >  1:264148/1‑107 (MQ=255)

                                                                                  cGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGaa                                                                              <  2:264148/107‑1 (MQ=255)

                                                                                    gTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGa                                            <  2:1529361/139‑1 (MQ=255)

                                                                                                  tttttCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAAt                              <  2:746603/139‑1 (MQ=255)

                                                                                                   ttttCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATa                             >  2:1549011/1‑139 (MQ=255)

                                                                                                       cGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCAt                         <  1:1549011/139‑1 (MQ=255)

                                                                                                           cATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAact                     >  2:172708/1‑139 (MQ=255)

                                                                                                                 tCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaa               <  1:172708/139‑1 (MQ=255)

                                                                                                                        aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGc                                                                                                <  2:1158640/51‑1 (MQ=255)

                                                                                                                        aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGc                                                                                                >  1:1158640/1‑51 (MQ=255)

                                                                                                                        aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATAtt                                 <  1:1203459/114‑1 (MQ=255)

                                                                                                                        aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATAtt                                 >  2:1203459/1‑114 (MQ=255)

                                                                                                                        aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATa        >  1:132092/1‑139 (MQ=255)

                                                                                                                        aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATa        >  1:1231417/1‑139 (MQ=255)

                                                                                                                        aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATa        >  2:1587506/1‑139 (MQ=255)

                                                                                                                         tGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGaa                                                                              >  1:759581/1‑68 (MQ=255)

                                                                                                                         tGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGaa                                                                              <  2:759581/68‑1 (MQ=255)

                                                                                                                          ggtggTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATaaa      >  2:199822/5‑139 (MQ=255)

                                                                                                                          ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                       <  2:1320504/106‑1 (MQ=255)

                                                                                                                          ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                       >  1:1320504/1‑106 (MQ=255)

                                                                                                                          ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATaaa      >  1:247307/1‑139 (MQ=255)

                                                                                                                          ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATaa       >  1:272156/1‑138 (MQ=255)

                                                                                                                          ggTTGTAATAAATTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGGCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATaa       <  2:272156/138‑1 (MQ=255)

                                                                                                                           gTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCAt                         <  2:1073472/119‑1 (MQ=255)

                                                                                                                           gTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCAt                         >  1:1073472/1‑119 (MQ=255)

                                                                                                                              gTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGgtg  <  1:110108/139‑1 (MQ=255)

                                                                                                                               taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGc                                                                                                <  2:317838/44‑1 (MQ=255)

                                                                                                                               taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGc                                                                                                >  1:317838/1‑44 (MQ=255)

                                                                                                                               taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAAt                              <  2:148282/110‑1 (MQ=255)

                                                                                                                               taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAAt                              >  2:130706/1‑110 (MQ=255)

                                                                                                                               taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAAt                              >  1:148282/1‑110 (MQ=255)

                                                                                                                               taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAAt                              <  1:130706/110‑1 (MQ=255)

TCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTG  >  NC_000913/480199‑480463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A