Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	757	76.1	3598070	93.4%	3360597	108.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	480,334	C→T	125 (TAG→TAA)	tomB ←	Hha toxicity attenuator, conjugation‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	480,334	0	C	T	100.0%	318.1 / NA	97	125 (TAG→TAA)	tomB	Hha toxicity attenuator, conjugation‑related protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (49/48); total (49/48)

TCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGT  >  NC_000913/480199‑480464

tCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATTCTTCTACCTATGGTTGTAATAATTTTaa                                                                                                                                 <  2:1113418/139‑1 (MQ=255)

   gATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACaa                                                                                                                              >  2:49116/1‑139 (MQ=255)

    aTAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAg                                                                                                                             >  2:857742/1‑139 (MQ=255)

              tttatttTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGc                                                                                                                   >  1:405736/1‑139 (MQ=255)

                                    tCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCg                                                                                                                    <  2:821127/116‑1 (MQ=255)

                                    tCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCg                                                                                                                    >  1:821127/1‑116 (MQ=255)

                                    tCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGa                                                                                                               <  2:65205/121‑1 (MQ=255)

                                    tCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGa                                                                                                               >  1:65205/1‑121 (MQ=255)

                                      cAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAAc                                                                                                                                >  1:409131/1‑102 (MQ=255)

                                      cAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAAc                                                                                                                                <  2:409131/102‑1 (MQ=255)

                                        gCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACaa                                                                                         <  2:405736/139‑1 (MQ=255)

                                         cGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACaaa                                                                                        >  2:196468/1‑139 (MQ=255)

                                           tgtCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAaac                                                                                      >  1:1579429/1‑139 (MQ=255)

                                             tCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAaacaa                                                                                    >  1:1687468/1‑139 (MQ=255)

                                              cAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGc                                                                                                 <  2:12646/125‑1 (MQ=255)

                                              cAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGc                                                                                                 >  1:12646/1‑125 (MQ=255)

                                              cAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACaaaa                                                                                       <  2:1021349/135‑1 (MQ=255)

                                              cAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACaaaa                                                                                       >  1:1021349/1‑135 (MQ=255)

                                               aaTTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACg                                                                                  <  1:49116/139‑1 (MQ=255)

                                                aTTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACgg                                                                                 >  2:888974/1‑139 (MQ=255)

                                                  tGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCACATTGACAAAACAACGGaa                                                                               <  2:480296/139‑1 (MQ=255)

                                                   gTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTTGTCGCATTGACAAAACAACGGAAt                                                                              <  1:857742/139‑1 (MQ=255)

                                                   gTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAAt                                                                              <  2:1687468/139‑1 (MQ=255)

                                                        gCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCg                                                                         >  2:1618785/1‑139 (MQ=255)

                                                         cAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGa                                                                        >  1:440867/1‑139 (MQ=255)

                                                         cAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGa                                                                        >  2:793072/1‑139 (MQ=255)

                                                              acgTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTaa                                                                                                                                 <  1:1684847/77‑1 (MQ=255)

                                                              acgTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTaa                                                                                                                                 >  2:1684847/1‑77 (MQ=255)

                                                              acgTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTAcc                                                                   >  2:634758/1‑139 (MQ=255)

                                                              acgTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTACGGACATACTTCTACCTATGGTTGTAATAATATTAACAAGATAAACTCGCAGGATTCTATTTCGTCGCATTGACAAAACAACGGAATAGTCGATTAcc                                                                   >  2:1093280/1‑139 (MQ=255)

                                                              acgTAAACGAATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTAcc                                                                   >  2:1204959/1‑139 (MQ=255)

                                                               cgTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCt                                                                  >  2:77310/1‑139 (MQ=255)

                                                                                 tCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGa                                                <  1:888974/139‑1 (MQ=255)

                                                                                  cGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTAc                                                                    <  1:1625178/118‑1 (MQ=255)

                                                                                  cGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTAc                                                                    >  2:1625178/1‑118 (MQ=255)

                                                                                   ggTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAg                                              <  1:1618785/139‑1 (MQ=255)

                                                                                    gTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGa                                             <  1:528398/139‑1 (MQ=255)

                                                                                      tttCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTtcgt                                                                                                    <  2:1216319/82‑1 (MQ=255)

                                                                                      tttCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTtcgt                                                                                                    >  1:1216319/1‑82 (MQ=255)

                                                                                       ttCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATcc                                          <  1:793072/139‑1 (MQ=255)

                                                                                       ttCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATcc                                          <  1:1533619/139‑1 (MQ=255)

                                                                                         cGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                        <  2:1579429/139‑1 (MQ=255)

                                                                                         cGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                        <  1:196468/139‑1 (MQ=255)

                                                                                         cGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                        <  1:1204959/139‑1 (MQ=255)

                                                                                          gTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGc                                       <  2:1615141/139‑1 (MQ=255)

                                                                                           ttAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCa                                      <  2:440867/139‑1 (MQ=255)

                                                                                                  tttttCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAAt                               <  1:60461/139‑1 (MQ=255)

                                                                                                   ttttCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACaaaa                                                                                       <  1:113107/82‑1 (MQ=255)

                                                                                                   ttttCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACaaaa                                                                                       >  2:113107/1‑82 (MQ=255)

                                                                                                    tttCGGACATACTTCTACCTATAGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATAc                             >  1:114652/1‑139 (MQ=255)

                                                                                                     ttCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGa                                                                        >  2:1096797/1‑95 (MQ=255)

                                                                                                     ttCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGa                                                                        <  1:1096797/95‑1 (MQ=255)

                                                                                                       cGGACATAGTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCAt                          <  1:1093280/139‑1 (MQ=255)

                                                                                                           cATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                        <  1:266760/121‑1 (MQ=255)

                                                                                                           cATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                        >  2:266760/1‑121 (MQ=255)

                                                                                                            aTACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAaacaa                                                                                    >  2:740998/1‑76 (MQ=255)

                                                                                                            aTACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAaacaa                                                                                    <  1:740998/76‑1 (MQ=255)

                                                                                                                 tCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaa                <  1:77310/139‑1 (MQ=255)

                                                                                                                 tCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCAGCATATTAATACCATAACTACTGaa                <  1:634758/139‑1 (MQ=255)

                                                                                                                        aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                        <  1:592059/108‑1 (MQ=255)

                                                                                                                        aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                        >  2:592059/1‑108 (MQ=255)

                                                                                                                        aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                        >  1:605632/1‑108 (MQ=255)

                                                                                                                        aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                        <  2:605632/108‑1 (MQ=255)

                                                                                                                        aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCa                                      <  2:868487/110‑1 (MQ=255)

                                                                                                                        aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCa                                      >  1:868487/1‑110 (MQ=255)

                                                                                                                        aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCAt                          >  1:1781947/1‑122 (MQ=255)

                                                                                                                        aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCAt                          <  2:1781947/122‑1 (MQ=255)

                                                                                                                        aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATa         >  2:120958/1‑139 (MQ=255)

                                                                                                                         tGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATaa        >  2:1585228/1‑139 (MQ=255)

                                                                                                                         tGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATaa        >  1:232310/1‑139 (MQ=255)

                                                                                                                          ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGaa                                                                               >  1:827225/1‑67 (MQ=255)

                                                                                                                          ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGaa                                                                               <  2:827225/67‑1 (MQ=255)

                                                                                                                          ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACt                                                              >  2:1758044/1‑84 (MQ=255)

                                                                                                                          ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACt                                                              <  1:1758044/84‑1 (MQ=255)

                                                                                                                          ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATaa                        >  2:1793020/1‑122 (MQ=255)

                                                                                                                          ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATaa                        <  1:1793020/122‑1 (MQ=255)

                                                                                                                          ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaacaa             <  2:1243821/133‑1 (MQ=255)

                                                                                                                          ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaacaa             >  1:1243821/1‑133 (MQ=255)

                                                                                                                          ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaa                >  1:1499235/1‑130 (MQ=255)

                                                                                                                          ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaa                <  2:1499235/130‑1 (MQ=255)

                                                                                                                          ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATaaa       >  1:504019/1‑139 (MQ=255)

                                                                                                                              gTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGgt    >  1:1663206/1‑138 (MQ=255)

                                                                                                                              gTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGgt    <  2:1663206/138‑1 (MQ=255)

                                                                                                                               taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGa                                                                                            <  1:1286356/49‑1 (MQ=255)

                                                                                                                               taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGa                                                                                            >  2:1286356/1‑49 (MQ=255)

                                                                                                                               taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATAtt                                  <  2:763751/107‑1 (MQ=255)

                                                                                                                               taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATAtt                                  >  1:763751/1‑107 (MQ=255)

                                                                                                                               taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCAt                          <  2:910962/115‑1 (MQ=255)

                                                                                                                               taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCAt                          >  1:910962/1‑115 (MQ=255)

                                                                                                                               taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATaa                        >  2:1617004/1‑117 (MQ=255)

                                                                                                                               taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATaa                        <  1:1617004/117‑1 (MQ=255)

                                                                                                                               taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGgtgt  >  2:1565063/1‑139 (MQ=255)

                                                                                                                               taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGgtgt  >  1:551536/1‑139 (MQ=255)

                                                                                                                                 ataatTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGaa                                               >  2:1657278/1‑92 (MQ=255)

                                                                                                                                 ataatTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGaa                                               <  1:1657278/92‑1 (MQ=255)

                                                                                                                                 ataatTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaa                <  2:1557509/123‑1 (MQ=255)

                                                                                                                                 ataatTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaa                >  1:1557509/1‑123 (MQ=255)

TCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGT  >  NC_000913/480199‑480464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A