Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	759	65.2	3386260	90.7%	3071337	101.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,684,971	G→A	G179G (GGC→GGT)	glyA ←	serine hydroxymethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,684,971	0	G	A	100.0%	190.3 / NA	60	G179G (GGC→GGT)	glyA	serine hydroxymethyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (29/31); total (29/31)

ACAACGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGGAG  >  NC_000913/2684840‑2685103

acaacGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAat                                                                                                                               <  1:166871/139‑1 (MQ=255)

  aacGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAatat                                                                                                                             >  2:384934/1‑139 (MQ=255)

      tGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCag                                                                                                                         >  1:1673070/1‑139 (MQ=255)

       gAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCaga                                                                                                                        >  1:143753/1‑139 (MQ=255)

             gAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAacc                                                                                                                  <  2:143753/139‑1 (MQ=255)

              aGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAacca                                                                                                                 >  1:184202/1‑139 (MQ=255)

                   ccGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGAt                                                                                                            >  1:1628269/1‑139 (MQ=255)

                      ggTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAAt                                                                                                         <  1:115529/139‑1 (MQ=255)

                       gTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGa                                                                                                                                 >  1:1634347/1‑114 (MQ=255)

                       gTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGa                                                                                                                                 <  2:1634347/114‑1 (MQ=255)

                           gggTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCAttt                                                                                                    <  2:184202/139‑1 (MQ=255)

                             gTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTc                                                                                                  <  2:985391/139‑1 (MQ=255)

                                     cagcagCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAacca                                                                                                                 <  2:992349/116‑1 (MQ=255)

                                     cagcagCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAacca                                                                                                                 >  1:992349/1‑116 (MQ=255)

                                                 ggCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTg                                                                              >  2:141941/1‑139 (MQ=255)

                                                  gCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGt                                                                             <  2:1561662/139‑1 (MQ=255)

                                                            tGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAat                                                                                                                               >  1:465847/1‑79 (MQ=255)

                                                            tGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAat                                                                                                                               <  2:465847/79‑1 (MQ=255)

                                                            tGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAac                                                                                                                   >  2:1640180/1‑91 (MQ=255)

                                                            tGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAac                                                                                                                   <  1:1640180/91‑1 (MQ=255)

                                                            tGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGAt                                                                   >  2:95281/1‑139 (MQ=255)

                                                            tGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGAt                                                                   <  2:1628269/139‑1 (MQ=255)

                                                                 cATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCg                                                              <  2:188406/139‑1 (MQ=255)

                                                                        aCGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTc                                                                        <  1:391137/122‑1 (MQ=255)

                                                                        aCGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTc                                                                        >  2:391137/1‑122 (MQ=255)

                                                                             cAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGa                                                  >  1:381428/1‑139 (MQ=255)

                                                                             cAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGa                                                  >  1:1423988/1‑139 (MQ=255)

                                                                               ggTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTgg                                                                                  >  1:152120/1‑105 (MQ=255)

                                                                               ggTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTgg                                                                                  <  2:152120/105‑1 (MQ=255)

                                                                                  aaGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGt                                             <  1:95281/139‑1 (MQ=255)

                                                                                  aaGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGt                                             <  2:381428/139‑1 (MQ=255)

                                                                                   aGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCg                                                              <  2:844386/121‑1 (MQ=255)

                                                                                   aGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCg                                                              >  1:844386/1‑121 (MQ=255)

                                                                                     cACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTgg                                                                                  >  2:371101/1‑99 (MQ=255)

                                                                                     cACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTgg                                                                                  <  1:371101/99‑1 (MQ=255)

                                                                                        cGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGa                                                                                                                                 >  2:851805/1‑49 (MQ=255)

                                                                                        cGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGa                                                                                                                                 <  1:851805/49‑1 (MQ=255)

                                                                                          aTGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGa                                     <  2:1423988/139‑1 (MQ=255)

                                                                                          aTGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGa                                     >  1:983799/1‑139 (MQ=255)

                                                                                               gTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGA‑CGGTAGCATCGATACCGt                               >  2:47442/1‑139 (MQ=255)

                                                                                                tCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTa                                                            <  1:616770/110‑1 (MQ=255)

                                                                                                tCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTa                                                            >  2:616770/1‑110 (MQ=255)

                                                                                                tCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGt                               >  1:209976/1‑139 (MQ=255)

                                                                                                          cACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGA‑CGGTAGCATCGATACCGTAAGGAACGAtg                    <  1:47442/139‑1 (MQ=255)

                                                                                                           aCGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGAtg                    <  2:1673070/139‑1 (MQ=255)

                                                                                                            cGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGaa                         <  1:935077/133‑1 (MQ=255)

                                                                                                            cGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGaa                         >  2:935077/1‑133 (MQ=255)

                                                                                                              cATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTgg                                                                                  >  1:940128/1‑74 (MQ=255)

                                                                                                              cATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTgg                                                                                  <  2:940128/74‑1 (MQ=255)

                                                                                                               aTTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGt                                             >  1:794423/1‑110 (MQ=255)

                                                                                                               aTTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGt                                             <  2:794423/110‑1 (MQ=255)

                                                                                                                ttttCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTa               <  1:384934/139‑1 (MQ=255)

                                                                                                                           tCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCt                                                                                >  1:1263099/1‑63 (MQ=255)

                                                                                                                           tCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCt                                                                                <  2:1263099/63‑1 (MQ=255)

                                                                                                                           tCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGAt                                                                   <  2:937387/76‑1 (MQ=255)

                                                                                                                           tCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGAt                                                                   >  1:937387/1‑76 (MQ=255)

                                                                                                                           tCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAg                                                           <  1:1519823/84‑1 (MQ=255)

                                                                                                                           tCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAg                                                           >  2:1519823/1‑84 (MQ=255)

                                                                                                                           tCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCgg    >  2:620589/1‑139 (MQ=255)

                                                                                                                             caccacACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGgag  <  2:209976/139‑1 (MQ=255)

ACAACGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGGAG  >  NC_000913/2684840‑2685103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A