Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I2 R1
|
760 |
52.7 |
2744488 |
90.2% |
2475528 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,684,971 |
G→A |
G179G (GGC→GGT) |
glyA ← |
serine hydroxymethyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,684,971 | 0 | G | A | 100.0%
| 126.2
/ NA
| 40 | G179G (GGC→GGT) | glyA | serine hydroxymethyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/19); total (21/19) |
AACGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTA > NC_000913/2684842‑2685090
|
aacGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAatat < 2:677928/139‑1 (MQ=255)
ggAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGa > 2:1280798/1‑122 (MQ=255)
ggAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGa < 1:1280798/122‑1 (MQ=255)
cGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCAtt > 1:1053645/1‑139 (MQ=255)
aCGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCt > 2:258337/1‑139 (MQ=255)
aCGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCt > 1:569979/1‑139 (MQ=255)
agcagcAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTgtgt > 1:683133/1‑139 (MQ=255)
ggCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTg < 1:258337/139‑1 (MQ=255)
cccGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAGACCACCGATAATCATTTTc < 2:912810/117‑1 (MQ=255)
cccGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTc > 1:912810/1‑117 (MQ=255)
tatCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTa < 2:1053645/139‑1 (MQ=255)
cAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGa > 1:1326569/1‑139 (MQ=255)
ggTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTa > 2:550656/1‑127 (MQ=255)
ggTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTa < 1:550656/127‑1 (MQ=255)
aTGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAac > 2:893021/1‑61 (MQ=255)
aTGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAac < 1:893021/61‑1 (MQ=255)
aTGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTgg < 1:503025/94‑1 (MQ=255)
aTGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTgg > 2:503025/1‑94 (MQ=255)
aTGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGa > 1:290497/1‑139 (MQ=255)
tGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTc < 1:1011671/74‑1 (MQ=255)
tGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTc > 2:1011671/1‑74 (MQ=255)
tCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGt > 1:1260406/1‑93 (MQ=255)
tCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGt < 2:1260406/93‑1 (MQ=255)
tttCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAac < 2:922425/48‑1 (MQ=255)
tttCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAac > 1:922425/1‑48 (MQ=255)
ttCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCtt < 2:1192553/69‑1 (MQ=255)
ttCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCtt > 1:1192553/1‑69 (MQ=255)
ttCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAAcc < 2:305936/139‑2 (MQ=255)
ttCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACg > 2:194128/1‑139 (MQ=255)
cACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCg < 2:1031554/98‑1 (MQ=255)
cACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCg > 1:1031554/1‑98 (MQ=255)
cACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGAt > 2:205943/1‑124 (MQ=255)
cACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGAt < 1:205943/124‑1 (MQ=255)
cGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAacc < 2:1287541/44‑1 (MQ=255)
cGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAacc > 1:1287541/1‑44 (MQ=255)
cGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCg > 2:1214930/1‑96 (MQ=255)
cGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCg < 1:1214930/96‑1 (MQ=255)
ttttCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTa < 2:1326569/139‑1 (MQ=255)
aGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTa > 1:704564/1‑130 (MQ=255)
aGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTa < 2:704564/130‑1 (MQ=255)
|
AACGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTA > NC_000913/2684842‑2685090
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
