Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	750	51.0	2595496	93.6%	2429384	106.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,684,971	G→A	G179G (GGC→GGT)	glyA ←	serine hydroxymethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,684,971	0	G	A	100.0%	137.5 / NA	45	G179G (GGC→GGT)	glyA	serine hydroxymethyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/23); total (22/23)

AACAACGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGG  >  NC_000913/2684839‑2685101

aacaacGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGaa                                                                                                                              <  1:359060/139‑1 (MQ=255)

 acaacGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAat                                                                                                                             <  1:511186/139‑1 (MQ=255)

   aacGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAatat                                                                                                                           <  1:1027557/139‑1 (MQ=255)

   aacGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAatat                                                                                                                           >  1:604896/1‑139 (MQ=255)

     cGTGAGCATGAGGAACCGGATTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGc                                                                                                                         >  1:862702/1‑139 (MQ=255)

      gTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGa                                                                                                                               >  2:544830/1‑132 (MQ=255)

      gTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGa                                                                                                                               <  1:544830/132‑1 (MQ=255)

         aGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCagag                                                                                                                     >  1:1009745/1‑139 (MQ=255)

                       ggTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAAt                                                                                                       >  2:537289/1‑139 (MQ=255)

                         ttCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCa                                                                                                     >  1:175390/1‑139 (MQ=255)

                            gggTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCAttt                                                                                                  >  1:925328/1‑139 (MQ=255)

                              gTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTc                                                                                                <  2:925328/139‑1 (MQ=255)

                                  aCGCCAGCAGCAACCAGGCCCGCAACGTGCGCAATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCt                                                                                            >  2:767665/1‑139 (MQ=255)

                                   cGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCtt                                                                                           <  2:175390/139‑1 (MQ=255)

                                    gCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTtg                                                                                          <  2:1009745/139‑1 (MQ=255)

                                                            gTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGa                                                                  <  2:604896/139‑1 (MQ=255)

                                                                     atCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGc                                                                                             >  1:354125/1‑103 (MQ=255)

                                                                     atCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGc                                                                                             <  2:354125/103‑1 (MQ=255)

                                                                     atCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCACTCCACCACACCGGAATATGCAGAGAAACCACCGATAATATTTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAg                                                         >  1:230065/1‑139 (MQ=255)

                                                                              cAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGa                                                >  2:1051047/1‑139 (MQ=255)

                                                                                ggTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATa                                                                                                         <  2:852338/80‑1 (MQ=255)

                                                                                ggTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATa                                                                                                         >  1:852338/1‑80 (MQ=255)

                                                                                   aaGGACCGATGCTGTCAGCGATTTCACGCATTTTCGACCAGTCCACCACACCGGGATATGCAGAGAACCCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGt                                           <  2:230065/139‑1 (MQ=255)

                                                                                   aaGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGt                                           <  1:1051047/139‑1 (MQ=255)

                                                                                           aTGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGa                                                                                                                               <  2:659144/47‑1 (MQ=255)

                                                                                           aTGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGa                                                                                                                               >  1:659144/1‑47 (MQ=255)

                                                                                           aTGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCTAGATCGGCGTAGTCGATATGACCGGTAGCATCGa                                   >  1:722068/1‑139 (MQ=255)

                                                                                           aTGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGa                                   >  1:1066427/1‑139 (MQ=255)

                                                                                           aTGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGa                                   >  2:346138/1‑139 (MQ=255)

                                                                                           aTGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGa                                   >  1:1084150/1‑139 (MQ=255)

                                                                                                gTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCg                              >  2:502313/1‑139 (MQ=255)

                                                                                                 tCAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGt                             <  1:435887/139‑1 (MQ=255)

                                                                                                  cAGCGATTTCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTa                            <  1:537289/139‑1 (MQ=255)

                                                                                                       aTTTCACGCATTTTCGCCCAGTCCACCACATCGGAATATGCAGAGAAACCACCGATAATCATTTTGGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGaa                       <  2:862702/139‑1 (MQ=255)

                                                                                                          tCACGCATTTTCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGa                    <  1:346138/139‑1 (MQ=255)

                                                                                                                   ttCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACa           >  2:580089/1‑139 (MQ=255)

                                                                                                                    tCGCCCAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAg          <  2:1084150/139‑1 (MQ=255)

                                                                                                                        ccAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTa      <  2:277320/139‑1 (MQ=255)

                                                                                                                        ccAGTCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTa      <  1:616390/139‑1 (MQ=255)

                                                                                                                            tCCACCACACCGGAATATTCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCgg  <  1:767665/139‑1 (MQ=255)

                                                                                                                            tCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTc                                                                      >  2:900915/1‑71 (MQ=255)

                                                                                                                            tCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTc                                                                      <  1:900915/71‑1 (MQ=255)

                                                                                                                            tCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGaa                       >  1:692479/1‑118 (MQ=255)

                                                                                                                            tCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGaa                       <  2:692479/118‑1 (MQ=255)

                                                                                                                            tCCACCACACCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCgg  >  2:675428/1‑139 (MQ=255)

AACAACGTGAGCATGAGGAACCGGGTTCGGGTAGACGCCAGCAGCAACCAGGCCCGCAACGTGCGCCATATCAACGAACAGGTAAGCACCGATGCTGTCAGCGATTTCACGCATTTTCGCCCAGTCCACCACGCCGGAATATGCAGAGAAACCACCGATAATCATTTTCGGCTTGTGTTCTTTGGCTTGTTTTTCCAGATCGGCGTAGTCGATATGACCGGTAGCATCGATACCGTAAGGAACGATGTTGTACAGTTTACCGG  >  NC_000913/2684839‑2685101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A