Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	522,463	T→C	V683A (GTG→GCG)	ybbP →	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	522,463	0	T	C	100.0%	135.5 / NA	39	V683A (GTG→GCG)	ybbP	putative ABC transporter permease
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/20); total (19/20)

TTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTA  >  NC_000913/522330‑522591

ttCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGAtgg                                                                                                                             <  1:383371/139‑1 (MQ=255)

              tGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCAcc                                                                                                               <  1:799758/139‑1 (MQ=255)

                               cacaACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTAtgttgct                                                                                              <  2:246959/139‑1 (MQ=255)

                                     tCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATCGTGGTACTGGTCACCGCCTGCGGTATgttgctgttgct                                                                                        >  1:1034524/1‑139 (MQ=255)

                                       aaCCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTgg                                                                                      <  1:1011710/139‑1 (MQ=255)

                                                          ttAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGa                                                                     >  2:629774/1‑137 (MQ=255)

                                                          ttAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGa                                                                     <  1:629774/137‑1 (MQ=255)

                                                          ttAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGCCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAAt                                                                   >  2:1037893/1‑139 (MQ=255)

                                                                tGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgtca                                                             <  1:1115347/139‑1 (MQ=255)

                                                                         ttGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGc                                                                                                             <  1:856080/82‑1 (MQ=255)

                                                                         ttGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGc                                                                                                             >  2:856080/1‑82 (MQ=255)

                                                                            gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAgg                                                  <  2:248497/138‑1 (MQ=255)

                                                                            gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAgg                                                  >  1:248497/1‑138 (MQ=255)

                                                                            gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGa                                                 >  2:176230/1‑139 (MQ=255)

                                                                            gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGa                                                 >  1:118638/1‑139 (MQ=255)

                                                                            gcgcGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGa                                                 >  1:816992/1‑139 (MQ=255)

                                                                                gATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCtg                                             <  2:118638/139‑1 (MQ=255)

                                                                                 aTTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCtgg                                            <  1:1037893/139‑1 (MQ=255)

                                                                                            ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCAcaggt                                                                               <  2:420199/93‑1 (MQ=255)

                                                                                            ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCAcaggt                                                                               >  1:420199/1‑93 (MQ=255)

                                                                                            ggtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGgcgc                                 >  1:409649/1‑139 (MQ=255)

                                                                                            ggacggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGTTGGTAGTGGTCACCGCCTGCGGAATTTTGCTGTTGCTGCCACAGTTCCGGGGGGGATTGGCGCAGCGTCATCAGGAGCTGTCGGTGGGgccc                                 >  2:643369/4‑137 (MQ=255)

                                                                                             gtcggtcAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACGGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGca                                >  1:210508/1‑139 (MQ=255)

                                                                                                     ggTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgt                                                               <  1:580027/100‑1 (MQ=255)

                                                                                                     ggTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATgcgt                                                               >  2:580027/1‑100 (MQ=255)

                                                                                                           ggAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCTCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTgggc                                                                     <  1:683097/88‑2 (MQ=255)

                                                                                                           ggAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGa                                                                     >  2:683097/1‑88 (MQ=255)

                                                                                                            gAGCAGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGa                 >  1:45420/1‑139 (MQ=255)

                                                                                                                aGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTAtgttgctgttgc                                                                                         <  2:396731/63‑1 (MQ=255)

                                                                                                                aGGTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTAtgttgctgttgc                                                                                         >  1:396731/1‑63 (MQ=255)

                                                                                                                 ggTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGca                                <  1:772686/119‑1 (MQ=255)

                                                                                                                 ggTAAGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGca                                >  2:772686/1‑119 (MQ=255)

                                                                                                                    aaGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAActg         <  2:45420/139‑1 (MQ=255)

                                                                                                                    aaGTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAActg         <  1:176230/139‑1 (MQ=255)

                                                                                                                      gTCGGGCGCTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAActgct       <  1:39007/139‑1 (MQ=255)

                                                                                                                        cGGGCGCTGGAAGCGATGGTGGTACTGGTCACCACCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAActgct       <  2:617781/137‑1 (MQ=255)

                                                                                                                        cGGGCGCTGGAAGCGATGGTGGTACTGGTCACCACCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAActgct       >  1:617781/1‑137 (MQ=255)

                                                                                                                           gcgcTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTa  >  2:372888/1‑139 (MQ=255)

                                                                                                                            cgcTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACt                            <  2:391502/112‑1 (MQ=255)

                                                                                                                            cgcTGGAAGCGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACt                            >  1:391502/1‑112 (MQ=255)

TTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGGTCGGTCAGGTGCTGGAGCAGGTAAGTCGGGCGCTGGAAGTGATGGTGGTACTGGTCACCGCCTGCGGTATGTTGCTGTTGCTGGCACAGGTGCAGGTGGGAATGCGTCAGCGTCATCAGGAGCTGGTGGTGTGGCGCACACTCGGTGCGGGGAAAAAACTGCTGCGTA  >  NC_000913/522330‑522591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A