Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	854,765	G→A	373 (TAG→TAA)	ybiR →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	854,765	0	G	A	100.0%	137.9 / NA	44	373 (TAG→TAA)	ybiR	putative transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/20); total (24/20)

GCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAC  >  NC_000913/854630‑854900

gCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGt                                                                                                                                      >  1:34562/1‑139 (MQ=255)

                    tACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCg                                                                                                                   <  1:98761/138‑1 (MQ=255)

                    tACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCg                                                                                                                   >  2:98761/1‑138 (MQ=255)

                           ggCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGa                                                                                                           >  2:438720/1‑139 (MQ=255)

                                                                  ttCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGAc                                                                    >  1:535987/1‑139 (MQ=255)

                                                                       tACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGc                                                                                                                    >  2:1028712/1‑86 (MQ=255)

                                                                       tACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGc                                                                                                                    <  1:1028712/86‑1 (MQ=255)

                                                                       tACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAAcc                                                               >  2:517352/1‑139 (MQ=255)

                                                                        aCCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCg                                                              >  1:223579/1‑139 (MQ=255)

                                                                          cGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATaaaa                                                                                                                         >  1:1036605/1‑78 (MQ=255)

                                                                          cGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATaaaa                                                                                                                         <  2:1036605/78‑1 (MQ=255)

                                                                          cGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGc                                                            >  1:831548/1‑139 (MQ=255)

                                                                            aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCg                                                                                                                   >  1:322381/1‑82 (MQ=255)

                                                                            aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCg                                                                                                                   <  2:322381/82‑1 (MQ=255)

                                                                            aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTg                                                                                   >  2:605699/1‑114 (MQ=255)

                                                                            aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTg                                                                                   <  1:605699/114‑1 (MQ=255)

                                                                            aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGa                                                                  >  1:1105831/1‑131 (MQ=255)

                                                                            aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGa                                                                  <  2:1105831/131‑1 (MQ=255)

                                                                            aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCAt                                                          >  2:50213/1‑139 (MQ=255)

                                                                            aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCAt                                                          >  1:215631/1‑139 (MQ=255)

                                                                            aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCAt                                                          >  1:16407/1‑139 (MQ=255)

                                                                                   tgtgGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAg                                                   <  2:223579/139‑1 (MQ=255)

                                                                                       ggcggcGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGt                                               <  1:342814/139‑1 (MQ=255)

                                                                                       ggcggcGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACCAAGGTCTAGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAg                                                   >  2:1068277/1‑135 (MQ=255)

                                                                                       ggcggcGTTGGTGGGATATGTTTAGTTAGTTATACTCCCGGCCAACTAAGGTCTAGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAg                                                   <  1:1068277/135‑1 (MQ=255)

                                                                                         cggcgTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGa                                                                  <  1:1095508/118‑1 (MQ=255)

                                                                                         cggcgTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGa                                                                  >  2:1095508/1‑118 (MQ=255)

                                                                                            cgTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTa                                          >  1:199316/1‑139 (MQ=255)

                                                                                                 gtggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGc                                     >  1:1114648/1‑139 (MQ=255)

                                                                                                  tggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCt                                    <  2:831548/139‑1 (MQ=255)

                                                                                                  tggGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCt                                    <  1:50213/139‑1 (MQ=255)

                                                                                                           ttttGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCg                                                 <  1:26764/117‑1 (MQ=255)

                                                                                                           ttttGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCg                                                 >  2:26764/1‑117 (MQ=255)

                                                                                                               gttagttaTACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaa                          <  1:459272/136‑1 (MQ=255)

                                                                                                               gttagttaTACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaa                          >  2:459272/1‑136 (MQ=255)

                                                                                                                ttagttaTACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaacaac                      <  2:90780/139‑1 (MQ=255)

                                                                                                                     tataCTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGt                 <  2:199316/139‑1 (MQ=255)

                                                                                                                          tCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCt                                    <  2:1102437/115‑1 (MQ=255)

                                                                                                                          tCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCt                                    >  1:1102437/1‑115 (MQ=255)

                                                                                                                            ccGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAt                                                                              >  2:285653/1‑71 (MQ=255)

                                                                                                                            ccGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAt                                                                              <  1:285653/71‑1 (MQ=255)

                                                                                                                              ggCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTg        >  2:841953/1‑139 (MQ=255)

                                                                                                                                ccAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGc      <  1:517352/139‑1 (MQ=255)

                                                                                                                                    cTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAc  <  2:215631/139‑1 (MQ=255)

GCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTGGCTGAC  >  NC_000913/854630‑854900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A