Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I1 R1	770	68.5	3470224	91.7%	3182195	103.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	854,765	G→A	373 (TAG→TAA)	ybiR →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	854,765	0	G	A	100.0%	153.1 / NA	48	373 (TAG→TAA)	ybiR	putative transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (26/22); total (26/22)

AATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTG  >  NC_000913/854637‑854894

aaTTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCaa                                                                                                                         <  2:1121692/139‑1 (MQ=255)

          cgcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAgg                                                                                                               <  1:833434/139‑1 (MQ=255)

          cgcTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAgg                                                                                                               <  1:1652816/139‑1 (MQ=255)

                    ggCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATaaaaaa                                                                                                                 >  2:807951/1‑127 (MQ=255)

                    ggCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATaaaaaa                                                                                                                 <  1:807951/127‑1 (MQ=255)

                         aCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAgg                                                                                                               >  2:127767/1‑124 (MQ=255)

                         aCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAgg                                                                                                               <  1:127767/124‑1 (MQ=255)

                         aCGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGGTGTGGGCGGCGTTGGTGCGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCg                                                                                                >  2:1265349/1‑139 (MQ=255)

                             tCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCtt                                                                                            <  2:937311/139‑1 (MQ=255)

                               gccgcATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCtttt                                                                                          >  1:411422/1‑139 (MQ=255)

                                  gcATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCt                                                                                       <  2:1488816/139‑1 (MQ=255)

                                           ggCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGtttt                                                                              <  1:434268/139‑1 (MQ=255)

                                               tttCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAgg                                                                                                               <  1:159988/102‑1 (MQ=255)

                                               tttCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAgg                                                                                                               >  2:159988/1‑102 (MQ=255)

                                                          aTTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGa                                                               >  1:1101035/1‑139 (MQ=255)

                                                           ttCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGAc                                                              >  2:1542964/1‑139 (MQ=255)

                                                                   cGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGa                                                                         <  1:1173895/120‑1 (MQ=255)

                                                                   cGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGa                                                                         >  2:1173895/1‑120 (MQ=255)

                                                                   cGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCg                                                        >  1:1584449/1‑137 (MQ=255)

                                                                   cGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCg                                                        <  2:1584449/137‑1 (MQ=255)

                                                                     aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCg                                                                                                             >  2:428485/1‑82 (MQ=255)

                                                                     aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCg                                                                                                             <  1:428485/82‑1 (MQ=255)

                                                                     aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAg                                                                                                    <  2:289046/91‑1 (MQ=255)

                                                                     aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAg                                                                                                    >  1:289046/1‑91 (MQ=255)

                                                                     aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCAt                                                    >  1:481380/1‑139 (MQ=255)

                                                                     aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCAt                                                    >  1:182289/1‑139 (MQ=255)

                                                                     aTGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCAt                                                    >  2:154174/1‑139 (MQ=255)

                                                                          gttgtGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCgg                                               >  1:1378429/1‑139 (MQ=255)

                                                                            tgtgGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAg                                             <  1:1300808/139‑1 (MQ=255)

                                                                            tgtgGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCATTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAg                                             >  2:1300808/1‑139 (MQ=255)

                                                                                ggcggcGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTg                                                                             >  2:812877/1‑103 (MQ=255)

                                                                                ggcggcGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTg                                                                             <  1:812877/103‑1 (MQ=255)

                                                                                  cggcgTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAt                                                                        >  1:673917/1‑106 (MQ=255)

                                                                                  cggcgTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGAt                                                                        <  2:673917/106‑1 (MQ=255)

                                                                                     cgTTGGTGTGATATGTTTTGTTAGTTATACTCCCGCCCAACTAAGGTCTGGCCAATaaaa                                                                                                                   <  1:1061007/60‑1 (MQ=255)

                                                                                     cgTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATaaaa                                                                                                                   >  2:1061007/1‑60 (MQ=255)

                                                                                       ttGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGt                                                                                                   >  1:1109326/1‑74 (MQ=255)

                                                                                       ttGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGt                                                                                                   <  2:1109326/74‑1 (MQ=255)

                                                                                               atatGTTTTGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTtcat                          >  2:1197827/1‑139 (MQ=255)

                                                                                                    ttttGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGa                     >  1:450736/1‑139 (MQ=255)

                                                                                                    ttttGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCACGGAGCGGTTTTTAATCGCTTCATCAAGa                     >  2:1404274/1‑139 (MQ=255)

                                                                                                      ttGTTAGTTATACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaac                   <  1:154174/139‑1 (MQ=255)

                                                                                                         ttagttaTACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGaacaac                <  1:1235908/139‑1 (MQ=255)

                                                                                                             ttaTACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAg                                             >  2:1521274/1‑106 (MQ=255)

                                                                                                             ttaTACTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAg                                             <  1:1521274/106‑1 (MQ=255)

                                                                                                              tataCTCCCGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGt           <  2:1601069/139‑1 (MQ=255)

                                                                                                                      cGGCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCt   >  1:634036/1‑139 (MQ=255)

                                                                                                                       ggCCAACTAAGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTg  >  2:27752/1‑139 (MQ=255)

AATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAGGGTCTGGCAAATAAAAAAGGCGGATTATGAGTCCGCCTTTTGCTTTAAGTTTTGAAGATTAATTCAGACGAACCGGCATCCCGGAGCGGTTTTTAATCGCTTCATCAAGAACAACCTGGTCAACATCTG  >  NC_000913/854637‑854894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A