Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,735,484	T→C	V36A (GTC→GCC)	sodB →	superoxide dismutase, Fe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,735,484	0	T	C	100.0%	214.5 / NA	59	V36A (GTC→GCC)	sodB	superoxide dismutase, Fe
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (29/30); total (29/30)

ATAAAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTACTGGAACTGCCT  >  NC_000913/1735362‑1735619

ataaAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                         <  1:947979/139‑1 (MQ=255)

         agTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTaaa                                                                                                                >  1:646728/1‑139 (MQ=255)

         agTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTaaa                                                                                                                >  2:604707/1‑139 (MQ=255)

         agTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTaaa                                                                                                                >  1:374452/1‑139 (MQ=255)

           tAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAgg                                                                                                              >  2:878177/1‑139 (MQ=255)

           tAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAgg                                                                                                              <  1:878177/139‑1 (MQ=255)

              cAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTAc                                                                                                           <  1:604707/139‑1 (MQ=255)

              cAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTAc                                                                                                           >  2:721127/1‑139 (MQ=255)

                aTGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCTTCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACcg                                                                                                         <  2:646728/139‑1 (MQ=255)

                   tCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCa                                                                                                                                       <  2:1092705/106‑1 (MQ=255)

                   tCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTAc                                                                                                           <  2:878848/134‑1 (MQ=255)

                   tCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTAc                                                                                                           >  1:878848/1‑134 (MQ=255)

                   tCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGt                                                                                                      >  1:67619/1‑139 (MQ=255)

                           aTTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGt                                                                                              >  2:72646/1‑139 (MQ=255)

                                      tACCATATGCTTAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGa                                                                                   <  1:721127/139‑1 (MQ=255)

                                        ccATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTa                                                                                                                  >  1:477115/1‑106 (MQ=255)

                                        ccATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTa                                                                                                                  <  2:477115/106‑1 (MQ=255)

                                             tGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGAt                                                                                                                    <  1:998981/99‑1 (MQ=255)

                                             tGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGAt                                                                                                                    >  2:998981/1‑99 (MQ=255)

                                             tGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGatt                                                                            >  1:269226/1‑139 (MQ=255)

                                                  aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                         >  1:1037062/1‑89 (MQ=255)

                                                  aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                         <  2:1037062/89‑1 (MQ=255)

                                                  aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGAt                                                                                                                    >  2:97541/1‑94 (MQ=255)

                                                  aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGAt                                                                                                                    <  1:97541/94‑1 (MQ=255)

                                                       gCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGct                                                                  >  1:602691/1‑139 (MQ=255)

                                                        ctctGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACt                                                                                                                                     <  2:445016/71‑1 (MQ=255)

                                                        ctctGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACt                                                                                                                                     >  1:445016/1‑71 (MQ=255)

                                                        ctctGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGctc                                                                 <  2:602691/139‑1 (MQ=255)

                                                         tctGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGctct                                                                >  1:1077522/1‑139 (MQ=255)

                                                                ccGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTg                                                         <  1:871002/139‑1 (MQ=255)

                                                                  gCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGc                                                       <  2:177097/139‑1 (MQ=255)

                                                                   cacaTTTCTGCGGAAACCATCGAGTATCGCTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                         >  1:688995/1‑72 (MQ=255)

                                                                   cacaTTTCTGCGGAAACCATCGAGTATCGCTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGttt                                                                                                    >  1:688487/1‑93 (MQ=255)

                                                                   cacaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                         <  2:688995/72‑1 (MQ=255)

                                                                   cacaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTAc                                                                                                           >  2:310259/1‑86 (MQ=255)

                                                                   cacaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTAc                                                                                                           <  1:310259/86‑1 (MQ=255)

                                                                   cacaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGttt                                                                                                    <  2:688487/93‑1 (MQ=255)

                                                                       tttCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCa                                                                                                                                       <  2:839521/54‑1 (MQ=255)

                                                                       tttCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCa                                                                                                                                       >  1:839521/1‑54 (MQ=255)

                                                                           tGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTcaac                                              >  1:898081/1‑139 (MQ=255)

                                                                                aaaCCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGc                                         >  1:1101733/1‑139 (MQ=255)

                                                                                  aCCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCa                                                                     >  2:456828/1‑109 (MQ=255)

                                                                                  aCCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCa                                                                     <  1:456828/109‑1 (MQ=255)

                                                                                  aCCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTg                                                         <  2:563790/121‑1 (MQ=255)

                                                                                  aCCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTg                                                         >  1:563790/1‑121 (MQ=255)

                                                                                    cATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCt                                     <  1:278384/139‑1 (MQ=255)

                                                                                          gTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTc                               <  2:67619/139‑1 (MQ=255)

                                                                                          gTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTc                               <  1:72646/139‑1 (MQ=255)

                                                                                                 tACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAAcc                        <  2:374452/139‑1 (MQ=255)

                                                                                                        aGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                         <  2:322311/35‑1 (MQ=255)

                                                                                                        aGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                         >  1:322311/1‑35 (MQ=255)

                                                                                                        aGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCg                                                                       <  2:885905/85‑1 (MQ=255)

                                                                                                        aGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCg                                                                       >  1:885905/1‑85 (MQ=255)

                                                                                                        aGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACttt                 >  1:636094/1‑139 (MQ=255)

                                                                                                               tCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCa                                                                     <  1:310012/80‑1 (MQ=255)

                                                                                                               tCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCa                                                                     >  2:310012/1‑80 (MQ=255)

                                                                                                                 aGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTACTGGaa        <  2:636094/139‑1 (MQ=255)

                                                                                                                 aGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTACTGGaa        <  2:898081/139‑1 (MQ=255)

                                                                                                                       aTGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTACTGGAACTGCCt  >  1:183393/1‑139 (MQ=255)

ATAAAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTACTGGAACTGCCT  >  NC_000913/1735362‑1735619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A