Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,735,484	T→C	V36A (GTC→GCC)	sodB →	superoxide dismutase, Fe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,735,484	0	T	C	100.0%	244.3 / NA	68	V36A (GTC→GCC)	sodB	superoxide dismutase, Fe
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (35/33); total (35/33)

GCAAATTAATAATAAAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTACTGGAACTGCCT  >  NC_000913/1735351‑1735619

gCAAATTAATAATAAAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTa                                                                                                                                    >  1:1096687/1‑139 (MQ=255)

gCAAATTAATAATAAAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTa                                                                                                                                    >  2:493533/1‑139 (MQ=255)

gCAAATTAATAATAAAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGAACCATCAGACTTATGCCACTa                                                                                                                                    >  2:715870/1‑139 (MQ=255)

            taaAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAAcc                                                                                                                        <  2:1082437/139‑1 (MQ=255)

              aaGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTg                                                                                                                      <  1:901723/139‑1 (MQ=255)

              aaGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTg                                                                                                                      <  1:611831/139‑1 (MQ=255)

               aGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCa                                                                                                                                       <  2:1094274/121‑1 (MQ=255)

                  agagTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTa                                                                                                                  <  2:1096687/139‑1 (MQ=255)

                   gagTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCTGACTTATGCCACTAACCTGAACAACCTGATTaa                                                                                                                 >  1:884439/1‑139 (MQ=255)

                    agTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTaaa                                                                                                                >  2:51696/1‑139 (MQ=255)

                      tAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAgg                                                                                                              >  2:986541/1‑139 (MQ=255)

                      tAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAgg                                                                                                              >  2:51809/1‑139 (MQ=255)

                           aTGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACcg                                                                                                         >  2:933624/1‑139 (MQ=255)

                                aTTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCGCCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGttt                                                                                                    >  2:816511/1‑139 (MQ=255)

                                aTTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTACAGGTACCGCGttt                                                                                                    <  1:816511/139‑1 (MQ=255)

                                  tCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTaa                                                                                                                                   <  1:1032533/106‑1 (MQ=255)

                                  tCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTaa                                                                                                                                   >  2:1032533/1‑106 (MQ=255)

                                          ccTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAAt                                                                                          >  1:838365/1‑139 (MQ=255)

                                                        tGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaa                                                                                                                             >  1:1184310/1‑90 (MQ=255)

                                                        tGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGaa                                                                                                                             <  2:1184310/90‑1 (MQ=255)

                                                             aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCg                                                                       >  1:233511/1‑139 (MQ=255)

                                                             aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCg                                                                       <  1:1140632/139‑1 (MQ=255)

                                                             aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCg                                                                       <  1:829896/139‑1 (MQ=255)

                                                             aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCg                                                                       >  1:414395/1‑139 (MQ=255)

                                                             aaGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCg                                                                       >  2:829896/1‑139 (MQ=255)

                                                                  gCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTg                                                                                                   <  2:888982/106‑1 (MQ=255)

                                                                  gCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTg                                                                                                   >  1:888982/1‑106 (MQ=255)

                                                                  gCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGct                                                                  >  2:908432/1‑139 (MQ=255)

                                                                   ctctGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGctc                                                                 >  2:138950/1‑139 (MQ=255)

                                                                     ctGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTg                                                               >  1:1106448/1‑139 (MQ=255)

                                                                        gCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAg                                                            >  2:284986/1‑139 (MQ=255)

                                                                        gCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAg                                                            <  2:838365/139‑1 (MQ=255)

                                                                        gCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAg                                                            <  1:51809/139‑1 (MQ=255)

                                                                        gCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAg                                                            <  2:1106448/139‑1 (MQ=255)

                                                                           ccGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTg                                                         <  2:233511/139‑1 (MQ=255)

                                                                              cacaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAAcc                                                                                                                        <  2:483308/73‑1 (MQ=255)

                                                                              cacaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAAcc                                                                                                                        >  1:483308/1‑73 (MQ=255)

                                                                              cacaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCa                                                                     <  2:571694/124‑1 (MQ=255)

                                                                              cacaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCg                                                      >  1:107039/1‑139 (MQ=255)

                                                                              cacaTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTGGAAGGTAAATCACTGGAAGAGATTATTCGCa                                                                     >  1:571694/1‑124 (MQ=255)

                                                                                caTTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTa                                                    <  1:908432/139‑1 (MQ=255)

                                                                                   ttCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTc                                                 >  1:385173/1‑139 (MQ=255)

                                                                                        cGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTcaacaa                                            >  1:509321/1‑139 (MQ=255)

                                                                                          gAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACg                                          <  1:284986/139‑1 (MQ=255)

                                                                                                aTCGAGTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTc                                    <  1:986541/139‑1 (MQ=255)

                                                                                                     gTATCACTACGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTc                               <  2:884439/139‑1 (MQ=255)

                                                                                                         cACTACGGCAAGCACCATCAGACTTATGCCACTAAc                                                                                                                                  >  1:773980/1‑36 (MQ=255)

                                                                                                         cACTACGGCAAGCACCATCAGACTTATGCCACTAAc                                                                                                                                  <  2:773980/36‑1 (MQ=255)

                                                                                                            tACGGCAAGCACCATCAGACCTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAAcc                        <  1:1093379/139‑1 (MQ=255)

                                                                                                              cGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTa                                                    <  1:1036232/109‑1 (MQ=255)

                                                                                                              cGGCAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTa                                                    >  2:1036232/1‑109 (MQ=255)

                                                                                                                gCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                         <  1:452592/38‑1 (MQ=255)

                                                                                                                gCAAGCACCATCAGACTTATGCCACTAACCTGaacaac                                                                                                                         >  2:452592/1‑38 (MQ=255)

                                                                                                                gCAAGCACCATCAGACTTATGCCACTAACCTGaacaa                                                                                                                          <  2:55372/37‑1 (MQ=255)

                                                                                                                gCAAGCACCATCAGACTTATGCCACTAACCTGaacaa                                                                                                                          >  1:55372/1‑37 (MQ=255)

                                                                                                                 cAAGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACt                   <  1:51696/139‑1 (MQ=255)

                                                                                                                  acgCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGGTCAGGTCTGGAACCATACtt                  <  1:715870/137‑1 (MQ=255)

                                                                                                                   aGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTg                                                         <  2:769029/99‑1 (MQ=255)

                                                                                                                   aGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTg                                                         >  1:769029/1‑99 (MQ=255)

                                                                                                                   aGCACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACttt                 >  2:952493/1‑139 (MQ=255)

                                                                                                                     cACCATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCt               >  1:418501/1‑139 (MQ=255)

                                                                                                                        cATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCt                                     <  2:2848/114‑1 (MQ=255)

                                                                                                                        cATCAGACTTATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAAAGCAGCt                                     >  1:2848/1‑114 (MQ=255)

                                                                                                                                ttATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTa                                                    >  2:191861/1‑91 (MQ=255)

                                                                                                                                ttATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTa                                                    <  1:191861/91‑1 (MQ=255)

                                                                                                                                 tATGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTACTGGAACTGcc   <  2:418501/139‑1 (MQ=255)

                                                                                                                                  aTGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCa                                                                     <  1:755550/72‑1 (MQ=255)

                                                                                                                                  aTGCCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTACTGGAACTGCCt  >  1:309395/1‑139 (MQ=255)

GCAAATTAATAATAAAGGAGAGTAGCAATGTCATTCGAATTACCTGCACTACCATATGCTAAAGATGCTCTGGCACCGCACATTTCTGCGGAAACCATCGAGTATCACTACGGCAAGCACCATCAGACTTATGTCACTAACCTGAACAACCTGATTAAAGGTACCGCGTTTGAAGGTAAATCACTGGAAGAGATTATTCGCAGCTCTGAAGGTGGCGTATTCAACAACGCAGCTCAGGTCTGGAACCATACTTTCTACTGGAACTGCCT  >  NC_000913/1735351‑1735619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A