Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
781 |
46.9 |
2231650 |
93.5% |
2086592 |
109.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,817,796 |
(T)6→5 |
coding (705/1353 nt) |
chbF ← |
phospho‑chitobiase, general 6‑phospho‑beta‑glucosidase activity |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,817,791 | 0 | T | . | 100.0%
| 246.3
/ NA
| 58 | coding (710/1353 nt) | chbF | phospho‑chitobiase, general 6‑phospho‑beta‑glucosidase activity |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (28/30); total (28/30) |
GCTCGTGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATC > NC_000913/1817653‑1817924
|
gcTCGTGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCGGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAatat > 2:582453/1‑139 (MQ=255)
gcgcCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATAtttttac > 2:712449/1‑137 (MQ=255)
gccTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCGGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAgg < 1:582453/139‑1 (MQ=255)
cTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGAc < 1:57281/139‑1 (MQ=255)
ttCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACt > 2:426403/1‑139 (MQ=255)
cGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAatat > 2:635805/1‑114 (MQ=255)
cGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAatat < 1:635805/114‑1 (MQ=255)
gCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGcc > 2:80028/1‑139 (MQ=255)
ccATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCt < 1:712449/139‑1 (MQ=255)
tCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCACTGACGc > 2:443007/1‑139 (MQ=255)
cAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGcc < 2:929669/139‑1 (MQ=255)
tAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCcaca < 1:426403/139‑1 (MQ=255)
aGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATc > 2:68934/1‑139 (MQ=255)
aGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATc > 1:960875/1‑139 (MQ=255)
aTCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGccc > 2:658475/1‑121 (MQ=255)
aTCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGccc < 1:658475/121‑1 (MQ=255)
ttGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCaaa > 1:549205/1‑139 (MQ=255)
gcagATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCTGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAg > 1:752298/1‑114 (MQ=255)
gcagATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAg < 2:752298/114‑1 (MQ=255)
cagATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGa < 2:293261/80‑1 (MQ=255)
cagATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGa > 1:293261/1‑80 (MQ=255)
cagATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCca < 1:350208/103‑1 (MQ=255)
cagATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCca > 2:350208/1‑103 (MQ=255)
gATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCAttt > 1:416200/1‑139 (MQ=255)
aTAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTa < 2:1025989/139‑1 (MQ=255)
aTAAGAACATGACAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTa > 1:191722/1‑139 (MQ=255)
tAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTAt < 2:416200/139‑1 (MQ=255)
aCATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGca < 2:960875/139‑1 (MQ=255)
aCATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGca < 1:345518/139‑1 (MQ=255)
tGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGca < 1:975366/136‑1 (MQ=255)
tGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGca > 2:975366/1‑136 (MQ=255)
ggCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCcaca > 1:905052/1‑92 (MQ=255)
ggCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCcaca < 2:905052/92‑1 (MQ=255)
ggCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATc > 1:250430/1‑139 (MQ=255)
ggCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATc > 1:974081/1‑139 (MQ=255)
cagATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAgg > 2:517857/1‑61 (MQ=255)
cagATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAgg < 1:517857/61‑1 (MQ=255)
aTTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGa > 1:12098/1‑139 (MQ=255)
ttCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGaa < 1:68934/139‑1 (MQ=255)
ttCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGaa < 1:80028/139‑1 (MQ=255)
gaacgaaTTAAGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCat < 1:443007/139‑1 (MQ=255)
aGCCCTCACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAgg < 2:549205/139‑1 (MQ=255)
cACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATcc < 1:1015088/109‑1 (MQ=255)
cACTAAATGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATcc > 2:1015088/1‑109 (MQ=255)
aTGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCg < 2:191722/139‑1 (MQ=255)
tGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGcac < 2:283717/98‑1 (MQ=255)
tGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGcac > 1:283717/1‑98 (MQ=255)
tGGCAGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCga < 1:976196/139‑1 (MQ=255)
aGATCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCgataga > 1:985054/1‑139 (MQ=255)
aTCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATcc > 2:44942/1‑96 (MQ=255)
aTCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATcc < 1:44942/96‑1 (MQ=255)
aTCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAAt > 1:162827/1‑101 (MQ=255)
aTCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAAt < 2:162827/101‑1 (MQ=255)
aTCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGaa < 1:696106/104‑1 (MQ=255)
aTCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGaa > 2:696106/1‑104 (MQ=255)
aTCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGt < 1:64584/115‑1 (MQ=255)
aTCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGt > 2:64584/1‑115 (MQ=255)
aTCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCgatagata > 1:243980/1‑139 (MQ=255)
aTCGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCgatagata < 2:985054/139‑1 (MQ=255)
cGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGc < 2:731644/77‑1 (MQ=255)
cGAAAATA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGc > 1:731644/1‑77 (MQ=255)
aaaaTA‑TTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAatc > 1:647809/1‑139 (MQ=255)
|
GCTCGTGCGCCGCCTTTGTAGTATTCGCCCATTTCAATAGCCAGCATCTCTTTCTGCTTGAAGTAATACAGCAGATAAGAACATGGCAGCAGATTCAACGAACGAATTAAGCCCTCACTAAATGGCAGATCGAAAATATTTTTTACAGAGGACGCTTTTAACTGCCCTGACGCCACACCATCAAGCAATTCGGCAAAGCGCGACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATC > NC_000913/1817653‑1817924
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
