Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
781 |
46.9 |
2231650 |
93.5% |
2086592 |
109.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,931,373 |
C→T |
Q165* (CAG→TAG) |
purT → |
phosphoribosylglycinamide formyltransferase 2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,931,373 | 0 | C | T | 100.0%
| 73.6
/ NA
| 24 | Q165* (CAG→TAG) | purT | phosphoribosylglycinamide formyltransferase 2 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/10); total (14/10) |
CAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAAC > NC_000913/1931256‑1931503
|
cagctgcCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCag > 2:551891/1‑139 (MQ=255)
cagctgcCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGCTAGCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCag < 2:833983/139‑1 (MQ=255)
tccacttATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTc < 2:1030718/139‑1 (MQ=255)
cacttATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAg < 1:763133/139‑1 (MQ=255)
ttATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCa < 2:378628/139‑1 (MQ=255)
cGTTTTGCCGATAGGGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGa > 2:1101869/1‑139 (MQ=255)
ttCCGCGAGGCGGTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAg > 2:25514/1‑75 (MQ=255)
gCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCcgcg < 1:1101869/139‑1 (MQ=255)
tCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGc > 1:548453/1‑139 (MQ=255)
tCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGc > 1:615493/1‑139 (MQ=255)
tCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGc > 2:1006736/1‑139 (MQ=255)
tGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGcgtcg < 1:551891/139‑1 (MQ=255)
cATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTATTGAAGGCGTCGtt > 2:650025/1‑139 (MQ=255)
tAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGc < 2:878637/110‑1 (MQ=255)
tAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGc > 1:878637/1‑110 (MQ=255)
tAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGtt > 2:86242/1‑139 (MQ=255)
tAAAACCGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTCTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGtt > 1:781386/1‑139 (MQ=255)
cGGTGATGAGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACtt > 1:34523/1‑139 (MQ=255)
gatgaGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAg > 1:658024/1‑80 (MQ=255)
gatgaGCTCTTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAg < 2:658024/80‑1 (MQ=255)
ctctTCCGGCAAGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTCACTTCGAAATTAcc > 1:680751/1‑139 (MQ=255)
aaGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCggg > 2:540454/1‑83 (MQ=255)
aaGGGGTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCggg < 1:540454/83‑1 (MQ=255)
gggTAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAAc < 2:186194/134‑1 (MQ=255)
|
CAGCTGCCCACTTCCACTTATCGTTTTGCCGATAGCGAAAGCCTTTTCCGCGAGGCGGTTGCTGACATTGGCTATCCCTGCATTGTAAAACCGGTGATGAGCTCTTCCGGCAAGGGGCAGACGTTTATTCGTTCTGCAGAGCAACTTGCTCAGGCATGGAAGTACGCTCAGCAAGGCGGTCGCGCCGGAGCGGGCCGCGTAATTGTTGAAGGCGTCGTTAAGTTTGACTTCGAAATTACCCTGCTAAC > NC_000913/1931256‑1931503
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
