Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
781 |
46.9 |
2231650 |
93.5% |
2086592 |
109.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,525,930 |
C→T |
*309* (TAG→TAA) |
yfeR ← |
transcriptional regulator of yefH |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,525,930 | 0 | C | T | 100.0%
| 130.1
/ NA
| 41 | *309* (TAG→TAA) | yfeR | transcriptional regulator of yefH |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (25/16); total (25/16) |
ACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAA > NC_000913/2525794‑2526023
|
caacatcacacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTta > 1:997616‑M2/133‑139 (MQ=255)
tacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGa > 1:32619‑M2/117‑139 (MQ=255)
gggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATccc > 1:890680‑M2/113‑139 (MQ=255)
attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCtt > 1:115272‑M2/110‑139 (MQ=255)
attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCtt > 1:708217‑M2/110‑139 (MQ=255)
tatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGcc > 1:112219‑M2/104‑139 (MQ=255)
cggatgacaaactgggcgaagatttttacccggattcgataatctactccatcaaatttaacttctgatagattactgccgaggctcccgacaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCATCCATAAg > 2:429405‑M2/94‑139 (MQ=255)
cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAg < 2:32619‑M2/46‑1 (MQ=255)
gatgacatactgggcgaagatttttaccaggatgcgataatctactccatctaatttaacttctgattgattactgacggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCTCCCAGCCATTAgcg > 1:680741‑M2/92‑139 (MQ=255)
gatgacaaactgggcgaggatttttaccaggatgcgataatctactccatcaaatttaacttctgattgatgactgccgggactcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgcg > 2:636004‑M2/92‑139 (MQ=255)
gatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgcg > 2:232680‑M2/92‑139 (MQ=255)
actgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc < 1:423859‑M2/56‑1 (MQ=255)
actgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc > 2:1068676‑M2/84‑139 (MQ=255)
actgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc < 2:708217‑M2/56‑1 (MQ=255)
actgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc < 2:997616‑M2/56‑1 (MQ=255)
gcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc > 1:99929‑M2/79‑89 (MQ=255)
gcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc < 2:99929‑M2/11‑1 (MQ=255)
aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGAt > 2:445088‑M2/76‑90 (MQ=255)
aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGAt < 1:445088‑M2/15‑1 (MQ=255)
ttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCa < 1:232680‑M2/69‑1 (MQ=255)
ttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg < 2:115272‑M2/71‑1 (MQ=255)
ggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGcc < 2:68863‑M2/36‑1 (MQ=255)
ggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGcc > 1:68863‑M2/63‑98 (MQ=255)
atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTCATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCAc > 1:1090007‑M2/61‑130 (MQ=255)
atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTCATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCAc < 2:1090007‑M2/70‑1 (MQ=255)
ataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGAACCAGGTCCCATAAAGCtt > 2:476336‑M2/56‑139 (MQ=255)
tctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCTTTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgc > 1:849394‑M2/52‑139 (MQ=255)
catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa > 2:227617‑M2/45‑94 (MQ=255)
catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa < 1:227617‑M2/50‑1 (MQ=255)
catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTg > 2:44022‑M2/45‑139 (MQ=255)
tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg < 1:522123‑M2/89‑1 (MQ=255)
tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg > 2:522123‑M2/43‑131 (MQ=255)
caaatttgacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCGCGCCCAGCCATTAGCGCATTGCCCTGCTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGaa > 1:679144‑M2/42‑139 (MQ=255)
ttactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg > 2:321386‑M2/22‑110 (MQ=255)
ttactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg < 1:321386‑M2/89‑1 (MQ=255)
gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCAc < 2:759706‑M2/70‑1 (MQ=255)
gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCAc > 1:759706‑M2/17‑86 (MQ=255)
gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCAc < 2:739258‑M2/70‑1 (MQ=255)
gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCAc > 1:739258‑M2/17‑86 (MQ=255)
cccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa > 2:1098158‑M2/8‑57 (MQ=255)
cccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa < 1:1098158‑M2/50‑1 (MQ=255)
|
ACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAA > NC_000913/2525794‑2526023
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
