Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F1 I1 R1
|
757 |
76.1 |
3598070 |
93.4% |
3360597 |
108.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,525,930 |
C→T |
*309* (TAG→TAA) |
yfeR ← |
transcriptional regulator of yefH |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,525,930 | 0 | C | T | 100.0%
| 233.0
/ NA
| 71 | *309* (TAG→TAA) | yfeR | transcriptional regulator of yefH |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (35/36); total (35/36) |
ACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGG > NC_000913/2525794‑2526059
|
caacatcacacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTta > 1:2299‑M2/133‑139 (MQ=255)
acatcacacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTtata < 1:312448‑M2/9‑1 (MQ=255)
atcacacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc < 1:596063‑M2/11‑1 (MQ=255)
atcacacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc < 2:2299‑M2/11‑1 (MQ=255)
acacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGa < 1:949541‑M2/14‑1 (MQ=255)
acacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGa < 2:1667846‑M2/14‑1 (MQ=255)
ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATcccc > 2:988478‑M2/112‑139 (MQ=255)
ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATcccc > 1:1087170‑M2/112‑139 (MQ=255)
attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCtt > 2:440711‑M2/110‑139 (MQ=255)
attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCtt > 2:196872‑M2/110‑139 (MQ=255)
attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCtt < 1:440711‑M2/30‑1 (MQ=255)
tgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAtt > 1:1237541‑M2/96‑139 (MQ=255)
cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc > 1:844659‑M2/94‑104 (MQ=255)
cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc < 2:844659‑M2/11‑1 (MQ=255)
gatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgcg > 2:1668551‑M2/92‑139 (MQ=255)
tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa < 1:311701‑M2/50‑1 (MQ=255)
tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa < 1:23893‑M2/50‑1 (MQ=255)
actgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc < 1:699780‑M2/56‑1 (MQ=255)
actgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc < 1:196872‑M2/56‑1 (MQ=255)
gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAt > 2:614103‑M2/81‑123 (MQ=255)
gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAt < 1:614103‑M2/43‑1 (MQ=255)
gcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtc < 1:1424927‑M2/61‑1 (MQ=255)
cgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAAc < 1:559397‑M2/20‑1 (MQ=255)
cgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAAc > 2:559397‑M2/78‑97 (MQ=255)
cgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc < 1:1096443‑M2/56‑1 (MQ=255)
cgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc > 2:1096443‑M2/78‑133 (MQ=255)
cgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtct > 2:1585524‑M2/78‑139 (MQ=255)
cgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtct > 2:1076032‑M2/78‑139 (MQ=255)
tttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATTCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCAc < 1:406318‑M2/70‑1 (MQ=255)
tttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCAc < 2:1237541‑M2/70‑1 (MQ=255)
ttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg < 2:1087170‑M2/71‑1 (MQ=255)
taccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGt < 1:1076032‑M2/72‑1 (MQ=255)
ccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTcc < 1:1549973‑M2/74‑1 (MQ=255)
ggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATc < 2:1354415‑M2/25‑1 (MQ=255)
ggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATc > 1:1354415‑M2/63‑87 (MQ=255)
ggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTa < 2:1691421‑M2/45‑1 (MQ=255)
ggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTa > 1:1691421‑M2/63‑107 (MQ=255)
gatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATc < 2:427435‑M2/25‑1 (MQ=255)
gatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATc > 1:427435‑M2/62‑86 (MQ=255)
gatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc < 2:1749349‑M2/56‑1 (MQ=255)
gatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc > 1:1749349‑M2/62‑117 (MQ=255)
tctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATAc > 2:1739094‑M2/52‑68 (MQ=255)
tctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATAc < 1:1739094‑M2/17‑1 (MQ=255)
ctccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc > 2:795622‑M2/48‑103 (MQ=255)
ctccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc < 1:795622‑M2/56‑1 (MQ=255)
ctccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcggcg > 1:958390‑M2/48‑139 (MQ=255)
catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATAc < 2:1292938‑M2/17‑1 (MQ=255)
catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATAc > 1:1292938‑M2/45‑61 (MQ=255)
tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACa > 1:884663‑M2/43‑60 (MQ=255)
tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACa < 2:884663‑M2/18‑1 (MQ=255)
tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa > 2:666457‑M2/43‑139 (MQ=255)
tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGT‑‑‑ACGTCCCATAAAGCTTCAGCGGCGGTGGAAAg > 2:433596‑M2/43‑139 (MQ=255)
aacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgcgc < 1:1074784‑M2/49‑1 (MQ=255)
aacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgcgc > 2:1074784‑M2/35‑83 (MQ=255)
cttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACgg > 2:413646‑M2/33‑139 (MQ=255)
ttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGt < 1:666457‑M2/108‑1 (MQ=255)
ttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGt < 1:1503385‑M2/108‑1 (MQ=255)
gattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTg < 1:413646‑M2/116‑1 (MQ=255)
ttactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcg > 1:648233‑M2/22‑139 (MQ=255)
gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa > 1:518896‑M2/17‑66 (MQ=255)
gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa < 2:518896‑M2/50‑1 (MQ=255)
gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc < 1:708471‑M2/56‑1 (MQ=255)
gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc > 2:708471‑M2/17‑72 (MQ=255)
gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAATGGTTCTTCCTGCGCACCa > 1:1745478‑M2/17‑139 (MQ=255)
ccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAg > 2:234839‑M2/16‑139 (MQ=255)
cggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCa < 1:1457791‑M2/38‑1 (MQ=255)
cggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCa > 2:1457791‑M2/15‑52 (MQ=255)
cccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACt > 1:288303‑M2/8‑139 (MQ=255)
cccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACt > 1:817204‑M2/8‑139 (MQ=255)
cccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACt > 1:691628‑M2/8‑139 (MQ=255)
cggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAATGGTTCTTCCTGCGCACCAGCATCAACTgg < 2:1745478‑M2/134‑1 (MQ=255)
|
ACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGG > NC_000913/2525794‑2526059
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
