Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
715 |
38.4 |
1606192 |
96.6% |
1551581 |
120.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,525,930 |
C→T |
*309* (TAG→TAA) |
yfeR ← |
transcriptional regulator of yefH |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,525,930 | 0 | C | T | 100.0%
| 140.6
/ NA
| 44 | *309* (TAG→TAA) | yfeR | transcriptional regulator of yefH |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (23/21); total (23/21) |
ACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACT > NC_000913/2525794‑2526057
|
caacatcacacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTta > 2:189593‑M2/133‑139 (MQ=255)
caacatcacacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTta > 2:442318‑M2/133‑139 (MQ=255)
caacatcacacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTta > 1:297578‑M2/133‑139 (MQ=255)
acatcacacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTtata < 1:116464‑M2/9‑1 (MQ=255)
cacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGAt < 2:271800‑M2/15‑1 (MQ=255)
cattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATAc < 1:366565‑M2/17‑1 (MQ=255)
tactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAAc > 1:366916‑M2/120‑139 (MQ=255)
actacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATAAAACg > 1:162341‑M2/119‑139 (MQ=255)
ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACgg > 2:626395‑M2/112‑133 (MQ=255)
ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACgg < 1:626395‑M2/22‑1 (MQ=255)
ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATcccc > 2:25911‑M2/112‑139 (MQ=255)
attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACaa < 1:301931‑M2/19‑1 (MQ=255)
attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACaa > 2:301931‑M2/110‑128 (MQ=255)
attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCtt > 1:57214‑M2/110‑139 (MQ=255)
gatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATc > 2:661049‑M2/100‑124 (MQ=255)
gatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATc < 1:661049‑M2/25‑1 (MQ=255)
gatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGc > 1:204433‑M2/100‑139 (MQ=255)
cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGc > 2:333764‑M2/94‑128 (MQ=255)
cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGc < 1:333764‑M2/35‑1 (MQ=255)
gatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgcg < 2:366916‑M2/48‑1 (MQ=255)
ttggggAAGATTTTTAACAACATGCGATTATCTACTCCATCAAATTTAACTACTGATTGATTAGTGCAGGGGCTCCCGG‑‑‑‑‑‑GA‑‑‑CACTTTATATCTGATACAACGGATCCCCTACCCGCCCAGCCATTAGCGCATTGCCCTg < 1:765093/134‑1 (MQ=255)
gatgacaaactgggcgaagatttttacaggatgcgataatctactccctcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAAc > 1:574211‑M2/91‑110 (MQ=255)
gaggacaaactgggcgaagatttttacaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAAc < 2:574211‑M2/20‑1 (MQ=255)
tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa < 1:25911‑M2/50‑1 (MQ=255)
tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa < 2:204433‑M2/50‑1 (MQ=255)
tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa < 2:226338‑M2/50‑1 (MQ=255)
tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg < 2:97074‑M2/58‑1 (MQ=255)
ggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGt < 1:710732‑M2/60‑1 (MQ=255)
ggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGt > 2:793127‑M2/80‑139 (MQ=255)
ggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGt > 1:590414‑M2/80‑139 (MQ=255)
cgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtct < 2:145367‑M2/62‑1 (MQ=255)
agatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGt > 2:19285‑M2/75‑139 (MQ=255)
ttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCa < 1:189593‑M2/69‑1 (MQ=255)
ttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg < 1:442318‑M2/71‑1 (MQ=255)
ctccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCGCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcggcg < 1:545901‑M2/92‑1 (MQ=255)
tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa > 2:385957‑M2/43‑139 (MQ=255)
tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa > 2:440439‑M2/43‑139 (MQ=255)
tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa > 1:237181‑M2/43‑139 (MQ=255)
attgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttcttc > 2:586636‑M2/27‑139 (MQ=255)
actgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt < 2:505412‑M2/51‑1 (MQ=255)
actgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt > 1:505412‑M2/20‑70 (MQ=255)
gggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa > 2:674188‑M2/13‑62 (MQ=255)
gggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa < 1:674188‑M2/50‑1 (MQ=255)
cccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACt > 2:365966‑M2/8‑139 (MQ=255)
|
ACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACT > NC_000913/2525794‑2526057
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
