Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	750	51.0	2595496	93.6%	2429384	106.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,046,399	C→T	P488S (CCG→TCG)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,046,399	0	C	T	100.0%	89.7 / NA	29	P488S (CCG→TCG)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/13); total (16/13)

GAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAG  >  NC_000913/2046275‑2046533

gAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTc                                                                                                                          <  1:1272582/139‑1 (MQ=255)

      aCTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTAc                                                                                                                                <  1:1066854/127‑1 (MQ=255)

      aCTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTAc                                                                                                                                >  2:1066854/1‑127 (MQ=255)

        tGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTAc                                                                                                                  >  1:867535/1‑139 (MQ=255)

                                   cccTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCtt                                                                                                                                  <  1:1098831/96‑1 (MQ=255)

                                   cccTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCtt                                                                                                                                  >  2:1098831/1‑96 (MQ=255)

                                   cccTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGAt                                                                                       >  1:156825/1‑139 (MQ=255)

                                             cTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGt                                                                                 >  1:1285950/1‑135 (MQ=255)

                                             cTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGt                                                                                 <  2:1285950/135‑1 (MQ=255)

                                                taACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCAccgcc                                                                          >  2:740582/1‑139 (MQ=255)

                                                               cGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGc                                                           >  1:1034819/1‑139 (MQ=255)

                                                                       aaGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGGTTGAAGTCACCGCCGAAGATGTCGAACGCAATTTGTc                                                   <  2:867535/139‑1 (MQ=255)

                                                                       aaGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTc                                                   <  2:156825/139‑1 (MQ=255)

                                                                            gCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATc                                              >  1:71745/1‑139 (MQ=255)

                                                                             ccGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCg                                             <  1:740582/139‑1 (MQ=255)

                                                                                 tGGCAAAGTGGTCACACCGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGaa                                         <  1:626104/139‑1 (MQ=255)

                                                                                   gCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACa                                       <  2:71745/139‑1 (MQ=255)

                                                                                             cacaACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGaa                                         >  1:1149931/1‑127 (MQ=255)

                                                                                             cacaACGGGTAAAGATATTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGaa                                         <  2:1149931/127‑1 (MQ=255)

                                                                                                     gTAAAGATATTCTGGTTACCCTGTAGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCa                     <  1:720748/139‑1 (MQ=255)

                                                                                                           atatTCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCAcc               <  1:906174/139‑1 (MQ=255)

                                                                                                              ttCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTAc            >  1:949455/1‑139 (MQ=255)

                                                                                                               tCTGGTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACg           >  2:18986/1‑139 (MQ=255)

                                                                                                                   gTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGc                                                                                           >  1:568840/1‑55 (MQ=255)

                                                                                                                   gTTACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGc                                                                                           <  2:568840/55‑1 (MQ=255)

                                                                                                                     tACCCTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGc     >  2:1241440/1‑139 (MQ=255)

                                                                                                                        ccTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAg  >  2:1212084/1‑139 (MQ=255)

                                                                                                                        ccTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAg  >  2:1149948/1‑139 (MQ=255)

                                                                                                                        ccTGTCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAg  >  1:73688/1‑139 (MQ=255)

GAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAG  >  NC_000913/2046275‑2046533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A