Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
750 |
51.0 |
2595496 |
93.6% |
2429384 |
106.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,475,251 |
T→C |
Y90H (TAT→CAT) |
yfdR → |
CPS‑53 (KpLE1) prophage, conserved protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,475,251 | 0 | T | C | 100.0%
| 173.7
/ NA
| 49 | Y90H (TAT→CAT) | yfdR | CPS‑53 (KpLE1) prophage, conserved protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/24); total (25/24) |
TGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACTATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCA > NC_000913/2475121‑2475344
|
tgtCGCCCAACATGCGGTGCTTTGCAGCAAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATGAACgg > 1:803717/1‑139 (MQ=255)
tCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACTCCTTCTTCCTGACCATAAACg > 1:1047379/1‑136 (MQ=255)
tCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACTCCTTCTTCCTGACCATAAACg < 2:1047379/136‑1 (MQ=255)
tCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGAt > 2:926758/1‑139 (MQ=255)
cccAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGga > 1:739567/1‑139 (MQ=255)
tGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGaaaaa < 2:739567/139‑1 (MQ=255)
cTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAg < 2:401431/136‑1 (MQ=255)
cTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAg > 1:401431/1‑136 (MQ=255)
gCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAcctc < 1:292659/139‑1 (MQ=255)
ttttGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATg > 2:164244/1‑91 (MQ=255)
ttttGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATg < 1:164244/91‑1 (MQ=255)
cATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAg < 1:62517/139‑1 (MQ=255)
cATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAg < 1:926758/139‑1 (MQ=255)
tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGgaagaa < 1:432045/76‑1 (MQ=255)
tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGgaagaa > 2:432045/1‑76 (MQ=255)
tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAAt < 2:712918/81‑1 (MQ=255)
tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAAt > 1:712918/1‑81 (MQ=255)
tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCGGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCa < 1:309718/128‑1 (MQ=255)
tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAcct > 1:341899/1‑115 (MQ=255)
tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAcct < 1:927195/115‑1 (MQ=255)
tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAcct < 2:341899/115‑1 (MQ=255)
tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAcct > 2:927195/1‑115 (MQ=255)
tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGaaa < 1:672229/139‑1 (MQ=255)
tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGaaa > 1:524761/1‑139 (MQ=255)
tgCAACAGAAGCGTATTGCCAGGACATCCCCGCACCAATGCAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCa > 2:309718/1‑128 (MQ=255)
gCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGa < 1:1074038/74‑1 (MQ=255)
gCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGa > 2:1074038/1‑74 (MQ=255)
gCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTgag < 2:1066335/90‑1 (MQ=255)
gCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTgag > 1:1066335/1‑90 (MQ=255)
gCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGAtc > 1:997671/1‑139 (MQ=255)
tATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCa > 1:7086/1‑139 (MQ=255)
ggACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAc < 1:892817/92‑1 (MQ=255)
ggACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTAc > 2:892817/1‑92 (MQ=255)
gACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATg > 1:788889/1‑48 (MQ=255)
gACATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATg < 2:788889/48‑1 (MQ=255)
aCATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGga < 1:1162151/49‑1 (MQ=255)
aCATCCCCGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGga > 2:1162151/1‑49 (MQ=255)
ccccGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGtt > 1:565914/1‑84 (MQ=255)
ccccGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGtt < 2:565914/84‑1 (MQ=255)
cccGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGtt < 1:643516/83‑1 (MQ=255)
cccGCACCACTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGtt > 2:643516/1‑83 (MQ=255)
cTGAAACGCCTTCTTCCTGACCATAAACGGATGgaagaa < 1:218428/39‑1 (MQ=255)
cTGAAACGCCTTCTTCCTGACCATAAACGGATGgaagaa > 2:218428/1‑39 (MQ=38)
cTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAg > 2:1227150/1‑89 (MQ=255)
cTGAAACGCCTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAg < 1:1227150/89‑1 (MQ=255)
ccTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATAc < 1:733675/61‑1 (MQ=255)
ccTTCTTCCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATAc > 2:733675/1‑61 (MQ=255)
tcttcCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCa > 2:441625/1‑80 (MQ=255)
tcttcCTGACCATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCa < 1:441625/80‑1 (MQ=255)
|
TGTCGCCCAACATGCGGTGCTTTGCAGCCAGCTGGTGCCGCAGGAATTTGCTTTTGAAGCATTAATGCATGATGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACTATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCA > NC_000913/2475121‑2475344
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
