Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I1 R1 750 51.0 2595496 93.6% 2429384 106.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,602,017 (C)7→8 coding (200/2019 nt) hyfB → hydrogenase 4, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,602,0101.C100.0% 185.9 / NA 54A65P (GCC→CCC) hyfBhydrogenase 4, membrane subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (22/32);  total (22/32)

GCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATAT  >  NC_000913/2601875‑2602125
                                                                                                                                        |                                                                                                                   
gCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑ccc                                                                                                                  >  2:686708/1‑139 (MQ=255)
     gCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑cccccccc                                                                                                             >  2:466880/1‑138 (MQ=255)
     gCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑cccccccc                                                                                                             >  2:1245891/1‑138 (MQ=255)
      cTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑cccccccct                                                                                                            >  1:1181378/1‑137 (MQ=255)
                   gTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATAACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCg                                                                                                >  2:931621/1‑139 (MQ=255)
                        gACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTa                                                                                           <  1:931621/139‑1 (MQ=255)
                             aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAAccc                                                                                       >  2:135179/1‑138 (MQ=255)
                             aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAAccc                                                                                       <  1:135179/138‑1 (MQ=255)
                             aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCt                                                                                      >  2:540819/1‑139 (MQ=255)
                             aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCt                                                                                      >  1:583935/1‑139 (MQ=255)
                                ggCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCg                                                                                   <  1:1075834/139‑1 (MQ=255)
                                 gCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGa                                                                                  <  2:228196/139‑1 (MQ=255)
                                  cTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGaa                                                                                 >  1:1198097/1‑139 (MQ=255)
                                       aaGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGAt                                                                            <  2:1041160/139‑1 (MQ=255)
                                         gCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAg                                                                          <  2:1084653/139‑1 (MQ=255)
                                               cGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCtctc                                                                    <  2:583935/139‑1 (MQ=255)
                                               cGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCtctc                                                                    >  1:818242/1‑139 (MQ=255)
                                               cGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCtctc                                                                    >  2:441547/1‑139 (MQ=255)
                                                  cATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTATGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGc                                                                 <  2:373269/139‑1 (MQ=255)
                                                           gCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGt                                                        <  1:44090/139‑1 (MQ=255)
                                                            cTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTg                                                       >  1:505140/1‑139 (MQ=255)
                                                             tggtggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGc                                                      <  2:1198097/139‑1 (MQ=255)
                                                             tggtggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGc                                                      <  2:505140/139‑1 (MQ=255)
                                                                 gggCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGt                                                  <  2:1181378/139‑1 (MQ=255)
                                                                    cggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGt                                                  >  1:684805/1‑136 (MQ=255)
                                                                    cggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGt                                                  <  2:684805/136‑1 (MQ=255)
                                                                    cggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAt                                               <  2:192088/139‑1 (MQ=255)
                                                                       cgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAtctc                                            >  1:1222894/1‑139 (MQ=255)
                                                                         tGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCt                                          >  1:1072311/1‑139 (MQ=255)
                                                                           aTTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTg                                        <  1:441547/139‑1 (MQ=255)
                                                                            ttGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGc                                       <  2:1072311/139‑1 (MQ=255)
                                                                                 atcatcAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtg                                  <  1:725361/139‑1 (MQ=255)
                                                                                    atcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTa                                                            >  1:326862/1‑110 (MQ=255)
                                                                                    atcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTa                                                            <  2:326862/110‑1 (MQ=255)
                                                                                     tcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtggtgg                              >  1:1088206/1‑139 (MQ=255)
                                                                                      caGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTCAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTggtggtggt                             <  2:929957/139‑1 (MQ=255)
                                                                                             aTTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCctc                                                                      >  1:520135/1‑91 (MQ=255)
                                                                                             aTTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCctc                                                                      <  2:520135/91‑1 (MQ=255)
                                                                                                       tACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCCATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt            <  2:144537/139‑1 (MQ=255)
                                                                                                                gtgtAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCctct                                                                     <  2:757353/73‑1 (MQ=255)
                                                                                                                gtgtAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCctct                                                                     >  1:757353/1‑73 (MQ=255)
                                                                                                                gtgtAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtc                       >  2:659017/1‑119 (MQ=255)
                                                                                                                gtgtAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtc                       <  1:659017/119‑1 (MQ=255)
                                                                                                                gtgtAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCa             >  1:491363/1‑129 (MQ=255)
                                                                                                                gtgtAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCa             <  2:491363/129‑1 (MQ=255)
                                                                                                                gtgtAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTtata   <  1:540819/139‑1 (MQ=255)
                                                                                                                gtgtAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCCGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTtata   <  2:1088206/139‑1 (MQ=255)
                                                                                                                 tgtAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAAccc                                                                                       <  1:565077/54‑1 (MQ=255)
                                                                                                                 tgtAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAAccc                                                                                       >  2:565077/1‑54 (MQ=255)
                                                                                                                 tgtAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTatat  <  2:818242/139‑1 (MQ=255)
                                                                                                                   tAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAAccc                                                                                       >  1:1279967/1‑52 (MQ=255)
                                                                                                                   tAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAAccc                                                                                       <  2:1279967/52‑1 (MQ=255)
                                                                                                                          aGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTggtggtggt                             >  1:765256/1‑103 (MQ=255)
                                                                                                                          aGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTggtggtggt                             <  2:765256/103‑1 (MQ=255)
                                                                                                                               cccGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGc                                                                                    >  2:16198/1‑43 (MQ=255)
                                                                                                                               cccGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGc                                                                                    <  1:16198/43‑1 (MQ=255)
                                                                                                                               cccGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTg                                                       >  1:413850/1‑72 (MQ=255)
                                                                                                                               cccGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTg                                                       <  2:413850/72‑1 (MQ=255)
                                                                                                                                        |                                                                                                                   
GCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATAT  >  NC_000913/2601875‑2602125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A