Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,602,017	(C)_7→8	coding (200/2019 nt)	hyfB →	hydrogenase 4, membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,602,010	1	.	C	97.1%	113.4 / ‑3.2	34	coding (193/2019 nt)	hyfB	hydrogenase 4, membrane subunit
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (15/18); total (15/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

GCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATA  >  NC_000913/2601880‑2602124

gCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑cccccccc                                                                                                            >  1:969003/1‑138 (MQ=255)

  tGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCctt                                                                                                          <  1:698675/139‑1 (MQ=255)

                       gACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGACGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAAccc                                                                                      <  2:387593/139‑1 (MQ=255)

                        aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTaa                                                                                         >  2:21584/1‑135 (MQ=255)

                        aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTaa                                                                                         <  1:21584/135‑1 (MQ=255)

                        aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAAccc                                                                                      >  2:709463/1‑138 (MQ=255)

                        aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAAccc                                                                                      <  1:709463/138‑1 (MQ=255)

                        aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCt                                                                                     >  2:47017/1‑139 (MQ=255)

                         cTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGTGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑ccccc                                                                                                               >  1:291802/1‑111 (MQ=255)

                         cTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGTGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑ccccc                                                                                                               <  2:291802/111‑1 (MQ=255)

                             cTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGaa                                                                                <  1:496566/139‑1 (MQ=255)

                             cTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGaa                                                                                >  1:372164/1‑139 (MQ=255)

                                                          gtggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTg                                                   >  1:780402/1‑139 (MQ=255)

                                                            gggCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGt                                                 <  1:210920/139‑1 (MQ=255)

                                                                 gcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTaa                                                                                         <  2:970594/94‑1 (MQ=255)

                                                                 gcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTaa                                                                                         >  1:970594/1‑94 (MQ=255)

                                                                   gTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTcc                                          <  2:780402/139‑1 (MQ=255)

                                                                            atcatcAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtg                                 >  2:519995/1‑139 (MQ=255)

                                                                              catcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCctct                                                                    >  2:249382/1‑102 (MQ=255)

                                                                              catcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCctct                                                                    <  1:249382/102‑1 (MQ=255)

                                                                                tcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGc                                                                                                 >  2:884751/1‑71 (MQ=255)

                                                                                tcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGc                                                                                                 <  1:884751/71‑1 (MQ=255)

                                                                                         ttACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTg                                                                                                  >  2:384039/1‑61 (MQ=255)

                                                                                         ttACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTg                                                                                                  <  1:384039/61‑1 (MQ=255)

                                                                                          tACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctct                   <  2:372164/139‑1 (MQ=255)

                                                                                                 ttACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCa            <  2:969003/139‑1 (MQ=255)

                                                                                                       gCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTa                                               <  2:87111/98‑1 (MQ=255)

                                                                                                       gCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTa                                               >  1:87111/1‑98 (MQ=255)

                                                                                                           gtgtAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCgg                                                                 >  2:126478/1‑76 (MQ=255)

                                                                                                           gtgtAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCgg                                                                 <  1:126478/76‑1 (MQ=255)

                                                                                                           gtgtAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTtata  <  2:753645/139‑1 (MQ=255)

                                                                                                            tgtAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTa                                                           <  2:416230/81‑1 (MQ=255)

                                                                                                            tgtAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTa                                                           >  1:416230/1‑81 (MQ=255)

                                                                                                                 cTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAAttt                                                                                                   >  1:98419/1‑36 (MQ=255)

                                                                                                                 cTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAAttt                                                                                                   <  2:98419/36‑1 (MQ=255)

                                                                                                                              ttttGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                        <  2:303797/98‑1 (MQ=255)

                                                                                                                              ttttGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                        >  1:303797/1‑98 (MQ=255)

GCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATA  >  NC_000913/2601880‑2602124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A