Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	380,022	(G)_10→11	intergenic (‑141/+47)	frmR ← / ← yaiO	regulator protein that represses frmRAB operon/outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	380,012	1	.	G	83.6%	174.9 / 27.6	61	intergenic (‑131/+57)	frmR/yaiO	regulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand): ref base . (7/3); new base G (34/17); total (41/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.34e-01

GGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAC  >  NC_000913/379877‑380135

ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑ggg                                                                                                                            >  2:739071/1‑139 (MQ=255)

                    aaaagacagatatACAAAAGGGGGGTATTCTATATGTCAATGCATGCGCCCCTATAATAAAGAATGGTGGAAAAGAAAATTTATCCGGTGAATGTGGAAGGAAAAAAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAgg                                                                                                       <  2:927111/130‑1 (MQ=255)

                       catgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑GGGGG‑GGGCGGCTAATCGGCAGGGaa                                                                                                    >  2:175402/1‑139 (MQ=255)

                        atgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAg                                                                                                   >  2:765269/1‑139 (MQ=255)

                                 aCTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCgg                                                                                         >  1:214679/1‑139 (MQ=255)

                                      aGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAGGGGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATa                                                                                      >  2:743475/1‑139 (MQ=255)

                                      aGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAg                                                                                     >  2:330205/1‑139 (MQ=255)

                                        gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAgg                                                                                                  <  1:5836/124‑1 (MQ=255)

                                        gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAgg                                                                                                  >  2:5836/1‑124 (MQ=255)

                                        gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg                                                                                   >  1:276076/1‑139 (MQ=255)

                                        gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGGCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGcccctgttcttt                                                                                   >  1:927111/1‑131 (MQ=255)

                                          gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg                                                                                 >  2:1014513/1‑139 (MQ=255)

                                          gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg                                                                                 >  2:512181/1‑139 (MQ=255)

                                          gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg                                                                                 >  2:550516/1‑139 (MQ=255)

                                          gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCAAATGGGAAGGGAAGGCCGCCCCGGATAggggg                                                                                 >  1:442609/1‑139 (MQ=255)

                                          gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑ggggg‑ggg‑gggcta                                                                                                               <  2:881709/108‑5 (MQ=255)

                                          gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑ggggg‑ggg‑gggcta                                                                                                               >  1:881709/1‑104 (MQ=255)

                                          gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑ggggg‑g                                                                                                                        <  2:563338/100‑1 (MQ=255)

                                          gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑ggggg‑g                                                                                                                        >  1:563338/1‑100 (MQ=255)

                                             tATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                                                                             >  2:921645/1‑139 (MQ=255)

                                               ttCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAACGGGGGGCa                                                                            >  1:1010819/1‑139 (MQ=255)

                                                    tatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg                                                                       >  1:885935/1‑139 (MQ=255)

                                                       gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAt                                                                    >  2:979924/1‑139 (MQ=255)

                                                          aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAACCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAg                                                                 >  1:743864/1‑139 (MQ=255)

                                                           aTGCATACCCCCCTATAGAAAAAAACCCTGGGGAAAAAAATTTTTCCGGGGAAGGGGGTCGGAAAAAAAAGG‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGa                                                                <  2:1010819/139‑1 (MQ=255)

                                                            tGCTTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGaa                                                               >  1:717316/1‑139 (MQ=255)

                                                            tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGaa                                                               >  2:444360/1‑139 (MQ=255)

                                                            tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCAAACGGGAAGGGAAGGCCCCCCCGGATGGGGGGCGGCAGAAGGAATCAGaa                                                               >  2:1033538/1‑139 (MQ=255)

                                                            tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑ggggg‑g                                                                                                                        >  1:1001535/1‑82 (MQ=255)

                                                            tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑ggggg‑g                                                                                                                        <  2:1001535/82‑1 (MQ=255)

                                                            tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAt                                                              >  1:568721/1‑139 (MQ=255)

                                                                tACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGGCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTc                                                           <  1:739071/139‑1 (MQ=255)

                                                                          taGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGAc                                                 <  2:628346/139‑1 (MQ=255)

                                                                              atataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                            <  2:214679/139‑1 (MQ=255)

                                                                               tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                            >  1:873065/1‑139 (MQ=255)

                                                                               tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                            >  1:76900/1‑139 (MQ=255)

                                                                               tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                            >  1:343459/1‑139 (MQ=255)

                                                                               tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                            >  2:12370/1‑139 (MQ=255)

                                                                               tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCAGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGGGGCAGAAGGAATAAGAATTTCAGGGTAAGACGGCCt                                            >  2:787311/1‑139 (MQ=255)

                                                                                 aaaCACTGGAGAATAAAATTTAACCCGTGGATGGGGGTAAAAAACAAAAAGGGAAG‑‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                                          <  1:787311/138‑1 (MQ=255)

                                                                                  aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCa                                         >  2:818029/1‑139 (MQ=255)

                                                                                  aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCaa                                        >  2:106965/1‑139 (MQ=255)

                                                                                    cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAGGGGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGGGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCaa                                        >  1:202488/1‑139 (MQ=255)

                                                                                    cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg                                       >  2:356264/1‑139 (MQ=255)

                                                                                    cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg                                       >  2:131567/1‑139 (MQ=255)

                                                                                    cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑ggggg‑ggg‑gggctaa                                                                                                              >  1:834553/1‑62 (MQ=255)

                                                                                    cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑ggggg‑ggg‑gggctaa                                                                                                              <  2:834553/67‑6 (MQ=255)

                                                                                    cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑GGGGG‑GGG‑GGCTAATCGGCAGGGAGGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGt                                      >  2:957161/1‑139 (MQ=255)

                                                                                     acTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGCCGGGCTGCAAGt                                      >  1:773999/1‑139 (MQ=255)

                                                                                       tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg                                    >  2:312139/1‑139 (MQ=255)

                                                                                       tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑ggggg‑gg                                                                                                                       >  2:149321/1‑56 (MQ=255)

                                                                                       tGGAGAATAAAATTTATCCGGGGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑ggggg‑gg                                                                                                                       <  1:149321/56‑1 (MQ=255)

                                                                                         gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCGGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                                  >  2:365950/1‑139 (MQ=255)

                                                                                         gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                                  >  1:1026000/1‑139 (MQ=255)

                                                                                         gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                                  >  1:198232/1‑139 (MQ=255)

                                                                                           gaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGG‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa                                <  1:765269/139‑1 (MQ=255)

                                                                                           gaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa                                <  1:550516/139‑1 (MQ=255)

                                                                                              tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAAt                                                                                                              >  2:872759/1‑58 (MQ=255)

                                                                                              tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAAt                                                                                                              <  1:872759/58‑1 (MQ=255)

                                                                                              tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAt                                                                    >  2:1008209/1‑100 (MQ=255)

                                                                                              tAAAATTTATCCGGTGAATGTGGTCGGAAAAAAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAt                                                                    <  1:1008209/100‑1 (MQ=255)

                                                                                                   tttATCCGGTGAATGTGGTCGGAAAACAACGA‑GGAAAG‑GGGGG‑GGG‑GGCTCATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTAAACCGGGCTGCAAGTTGCAGGCCGTTaaa                        >  1:1027922/1‑139 (MQ=255)

                                                                                                      aTCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGa                                                  >  1:271820/1‑110 (MQ=255)

                                                                                                      aTCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGa                                                  <  2:271820/110‑1 (MQ=255)

                                                                                                      aTCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatc                     <  1:12370/139‑1 (MQ=255)

                                                                                                                tgtgGGCGGAAAACAAAGG‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTggg           <  1:1033538/139‑1 (MQ=255)

                                                                                                                 gtgGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTgggg          <  1:131567/139‑1 (MQ=255)

                                                                                                                 gtgGTCGGAAAACAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTgggg          <  1:979924/139‑1 (MQ=255)

                                                                                                                   ggTCCGAAAAGATAGA‑GGGAG‑‑GGGGGTTGG‑TGCTAATCGGCAGGTGAGGCCACCACAGATATCCGGCGGCATAAGTAAACATAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCTTTAAAAGCATCGGTTGGGgtg        <  2:1027922/139‑1 (MQ=255)

                                                                                                                     tCGGAAAACAAAGA‑GGAAA‑‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTcc                                                          >  2:589130/1‑86 (MQ=255)

                                                                                                                     tCGGAAAACAAAGA‑GGAAA‑‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTcc                                                          <  1:589130/86‑1 (MQ=255)

                                                                                                                       ggAAAACAAAGA‑GGAAAGGGGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCg     <  1:743475/139‑1 (MQ=255)

                                                                                                                        gAAAACGAAGA‑GGAAA‑‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCACGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAc  >  2:648412/1‑139 (MQ=255)

                                                                                                                         aaaaCAAAGG‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAc  <  1:356264/139‑1 (MQ=255)

                                                                                                                         aaaaCAAAGA‑GGAAAG‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGCCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAc  <  2:743864/139‑1 (MQ=255)

GGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGA‑GGAAA‑‑GGGGG‑GGG‑GGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAC  >  NC_000913/379877‑380135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A