Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,627,124	T→C	D86G (GAT→GGT)	dcp ←	dipeptidyl carboxypeptidase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,627,124	0	T	C	100.0%	167.3 / NA	46	D86G (GAT→GGT)	dcp	dipeptidyl carboxypeptidase II
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/23); total (23/23)

TCAAGCCCCAGGGATTCACGGCGCTGCCAGACAGCATCTACCCGCGCGAATAATTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCATCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAGTATTGTTGAAATCAGGCATTTGCGGGTTAAGCGCGATGGC  >  NC_000913/1626995‑1627248

tCAAGCCCCAGGGATTCACGGCGCTGCCAGACAGCATCTACCCGCGCGAATAATTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTAt                                                                                                                     <  1:535158/139‑1 (MQ=255)

     ccccAGGGATTCACGGCGCTGCCAGACAGCATCTACCCGCGCGAATAATTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGcgcc                                                                                                                >  2:570292/1‑139 (MQ=255)

          gggATTCACGGCGCTGCCAGACAGCATCTACCCGCGCGAATAATTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGt                                                                                                           <  1:151418/139‑1 (MQ=255)

              ttCACGGCGCTGCCAGACAGCATCTACCCGCGCGAATAATTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGATGCTGTAATTCACCATTGGTATGCGCCGCAGTCATc                                                                                                       >  2:810815/1‑139 (MQ=255)

                cACGGCGCTGCCAGACAGCATCTACCCGCGCGAATAATTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGc                                                                                                     >  2:526701/1‑139 (MQ=255)

                                  cATCTACCCGCGCGAATAATTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAAc                                                                                   >  2:503759/1‑139 (MQ=255)

                                    tCTACCCGCGCGAATAATTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGCCGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAAcgc                                                                                 >  1:984516/1‑139 (MQ=255)

                                           gcgcgAATAATTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGgtaag                                                                          <  1:328337/139‑1 (MQ=255)

                                            cgcgAATAATTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGgtaagt                                                                         >  2:556862/1‑139 (MQ=255)

                                             gcgAATAATTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGgtaagta                                                                        <  1:435873/139‑1 (MQ=255)

                                                  taatTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCGAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATtct                                                                   >  2:39420/1‑139 (MQ=255)

                                                   aatTCACCGTTCCGATAGATATCATTAGCCAGTTCCTCCAATTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATtctc                                                                  <  2:630995/139‑1 (MQ=255)

                                                   aatTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATtctc                                                                  <  2:984516/139‑1 (MQ=255)

                                                       cACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCt                                                              <  1:754699/139‑1 (MQ=255)

                                                           gTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAAcg                                                                                  <  1:1011833/115‑1 (MQ=255)

                                                           gTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAAcg                                                                                  >  2:1011833/1‑115 (MQ=255)

                                                           gTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGt                                                          >  1:890039/1‑139 (MQ=255)

                                                             tCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTc                                                        <  1:556862/139‑1 (MQ=255)

                                                             tCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTc                                                        <  1:570292/139‑1 (MQ=255)

                                                               agatagatATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTcc                                                       >  1:382395/1‑138 (MQ=255)

                                                               agatagatATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTcc                                                       <  2:382395/138‑1 (MQ=255)

                                                                gatagataTCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCa                                                                                                         >  1:1014230/1‑87 (MQ=255)

                                                                gatagataTCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCa                                                                                                         <  2:1014230/87‑1 (MQ=255)

                                                                   agataTCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCGAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGc                                                  <  1:39420/139‑1 (MQ=255)

                                                                   agataTCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGc                                                  <  1:503759/139‑1 (MQ=255)

                                                                   agataTCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGc                                                  <  1:526701/139‑1 (MQ=255)

                                                                      tatCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAg                                               >  1:991943/1‑139 (MQ=255)

                                                                      tatCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAg                                               >  1:343185/1‑139 (MQ=255)

                                                                         cATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGc                                                  <  1:1014949/133‑1 (MQ=255)

                                                                         cATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGc                                                  >  2:1014949/1‑133 (MQ=255)

                                                                         cATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAAt                                            >  1:354346/1‑139 (MQ=255)

                                                                           ttAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTgg                                                                                       >  1:1004574/1‑94 (MQ=255)

                                                                           ttAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTgg                                                                                       <  2:1004574/94‑1 (MQ=255)

                                                                           ttAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAg                                          >  1:236027/1‑139 (MQ=255)

                                                                           ttAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAg                                          >  1:152351/1‑139 (MQ=255)

                                                                                cAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAGTAttg                                     <  1:738956/139‑1 (MQ=255)

                                                                                   ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTcc                                                       >  2:355337/1‑118 (MQ=255)

                                                                                   ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTcc                                                       <  1:355337/118‑1 (MQ=255)

                                                                                   ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTACGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCgg                                                                               >  2:71650/1‑94 (MQ=255)

                                                                                   ttCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTACGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCgg                                                                               <  1:71650/94‑1 (MQ=255)

                                                                                             tCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAGTATTGTTGAAATCAGGCa                        >  2:430670/1‑139 (MQ=255)

                                                                                                  ggAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCt                                                              >  2:374877/1‑96 (MQ=255)

                                                                                                  ggAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCt                                                              <  1:374877/96‑1 (MQ=255)

                                                                                                  ggAAAACTGCTCGTCAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCCTTGTTCCAGTGCCAGAATAGTATTGTTGAAATCAGGCATTTGc                   <  2:343185/139‑1 (MQ=255)

                                                                                                                cAAGACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAGTATTGTTGAAATCAGGCATTTGCGGGTTAAGCGCGAt     <  2:152351/139‑1 (MQ=255)

                                                                                                                   gACGCTGTAATTCACCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAGTATTGTTGAAATCAGGCATTTGCGGGTTAAGCGCGATGgc  >  1:775094/1‑139 (MQ=255)

TCAAGCCCCAGGGATTCACGGCGCTGCCAGACAGCATCTACCCGCGCGAATAATTCACCGTTCAGATAGATATCATTAGCCAGTTCCGCCAGTTCAGCGGAAAACTGCTCGTCAAGACGCTGTAATTCATCATTGGTATGCGCCGCAGTCATCGCAAAAAAGACGCTGGTAACGCGGGTAAGTAATTCTCCGCTTTGTTCCAGTGCCAGAATAGTATTGTTGAAATCAGGCATTTGCGGGTTAAGCGCGATGGC  >  NC_000913/1626995‑1627248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A