Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,244,245	G→A	A112A (GCG→GCA)	yeiG →	S‑formylglutathione hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,244,245	0	G	A	100.0%	161.5 / NA	50	A112A (GCG→GCA)	yeiG	S‑formylglutathione hydrolase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/23); total (27/23)

GGCGGAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTG  >  NC_000913/2244116‑2244377

ggcggAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGTGAAAAAGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTAc                                                                                                                             <  1:546509/139‑1 (MQ=255)

ggcggAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTAc                                                                                                                             <  1:567765/139‑1 (MQ=255)

 gcggAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTAcc                                                                                                                            <  1:209063/139‑1 (MQ=255)

 gcggAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTAcc                                                                                                                            <  1:639688/139‑1 (MQ=255)

     aaCTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt                                                                                                                        <  2:949687/139‑1 (MQ=255)

                        gATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGAt                                                                                                     >  2:735553/1‑139 (MQ=255)

                         aTGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATg                                                                                                    >  1:273879/1‑139 (MQ=255)

                           gCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTAcc                                                                                                                            <  2:688111/113‑1 (MQ=255)

                           gCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTAcc                                                                                                                            >  1:688111/1‑113 (MQ=255)

                           gCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGaa                                                                                                  >  1:952369/1‑139 (MQ=255)

                                acacCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTAcc                                                                                             >  1:969517/1‑139 (MQ=255)

                                    cAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGgcg                                                                                         >  2:422386/1‑139 (MQ=255)

                                      gCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCt                                                                                       >  2:659543/1‑139 (MQ=255)

                                          gcgcgGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGtt                                                                                   <  1:903552/139‑1 (MQ=255)

                                          gcgcgGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGtt                                                                                   >  1:418318/1‑139 (MQ=255)

                                                cgAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCg                                                                             <  1:157727/139‑1 (MQ=255)

                                                           ccAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTc                                                                              <  1:722812/127‑1 (MQ=255)

                                                           ccAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTc                                                                              >  2:722812/1‑127 (MQ=255)

                                                               cgacgaTGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGc                                                              <  2:394366/139‑1 (MQ=255)

                                                                    aTGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTgcg                                                                                                         <  1:51732/91‑1 (MQ=255)

                                                                    aTGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTgcg                                                                                                         >  2:51732/1‑91 (MQ=255)

                                                                       gCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTg                                                      <  2:418318/139‑1 (MQ=255)

                                                                                 aGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGTCAACGCATTACCGGAt                                                                                                                        <  2:16391/63‑1 (MQ=255)

                                                                                 aGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt                                                                                                                        >  1:16391/1‑63 (MQ=255)

                                                                                 aGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGc                                            >  1:271318/1‑139 (MQ=255)

                                                                                 aGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGc                                            >  2:124413/1‑139 (MQ=255)

                                                                                    ccAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGt                                         <  1:971419/139‑1 (MQ=255)

                                                                                    ccAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATAACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGt                                         <  1:699339/139‑1 (MQ=255)

                                                                                        ggCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGATTTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTgcg                                                    >  2:668026/1‑124 (MQ=255)

                                                                                        ggCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTgcg                                                    <  1:668026/124‑1 (MQ=255)

                                                                                        ggCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACt                                     >  2:728288/1‑139 (MQ=255)

                                                                                                         tAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTg                                                      <  2:383511/105‑1 (MQ=255)

                                                                                                         tAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTg                                                      >  1:383511/1‑105 (MQ=255)

                                                                                                         tAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGt                    >  1:514032/1‑139 (MQ=255)

                                                                                                         tAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGt                    >  1:238393/1‑139 (MQ=255)

                                                                                                         tAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGt                    >  1:415847/1‑139 (MQ=255)

                                                                                                          aaTGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGAcc                                                          <  2:353217/100‑1 (MQ=255)

                                                                                                          aaTGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGAcc                                                          >  1:353217/1‑100 (MQ=255)

                                                                                                            tGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGt                    >  1:673273/1‑136 (MQ=255)

                                                                                                            tGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGt                    <  2:673273/136‑1 (MQ=255)

                                                                                                            tGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTgcg                 <  2:415847/139‑1 (MQ=255)

                                                                                                                aCGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTgcg                                                                                                         >  2:425611/1‑47 (MQ=255)

                                                                                                                aCGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTgcg                                                                                                         <  1:425611/47‑1 (MQ=255)

                                                                                                                aCGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGTATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGa             >  1:711406/1‑139 (MQ=255)

                                                                                                                 cGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATg                                >  2:996631/1‑119 (MQ=255)

                                                                                                                 cGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATg                                <  1:996631/119‑1 (MQ=255)

                                                                                                                 cGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGAt            >  2:861800/1‑139 (MQ=255)

                                                                                                                       cgccgTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACg                      <  2:729643/123‑1 (MQ=255)

                                                                                                                       cgccgTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACg                      >  1:729643/1‑123 (MQ=255)

                                                                                                                           gTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTg  >  2:514818/1‑139 (MQ=255)

GGCGGAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGGTGGTCACGGTGCGCTGATTATGGCGCTG  >  NC_000913/2244116‑2244377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A