Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I1 R1
|
770 |
68.5 |
3470224 |
91.7% |
3182195 |
103.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,244,245 |
G→A |
A112A (GCG→GCA) |
yeiG → |
S‑formylglutathione hydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,244,245 | 0 | G | A | 100.0%
| 160.2
/ NA
| 49 | A112A (GCG→GCA) | yeiG | S‑formylglutathione hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/28); total (21/28) |
GCGGAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGG > NC_000913/2244117‑2244349
|
gcggAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTAcc < 1:330652/139‑1 (MQ=255)
aaCTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt < 2:555450/139‑1 (MQ=255)
aaCTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt < 1:1704829/139‑1 (MQ=255)
tGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTAcc < 2:157126/125‑1 (MQ=255)
tGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTAcc > 1:157126/1‑125 (MQ=255)
gTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGAt < 1:120232/129‑1 (MQ=255)
gTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGAt > 2:120232/1‑129 (MQ=255)
gTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGcgcg < 1:78736/139‑1 (MQ=255)
gCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGaa > 2:898440/1‑139 (MQ=255)
cAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACg < 2:488110/97‑1 (MQ=255)
cAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACg > 1:488110/1‑97 (MQ=255)
gCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt > 1:844376/1‑106 (MQ=255)
gCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt < 2:844376/106‑1 (MQ=255)
cgcgGCGAAAAGGTTGCCAACGACGATGGCTACGTTTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTc > 2:249358/1‑139 (MQ=255)
ggcgAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt > 2:297302/1‑98 (MQ=255)
ggcgAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt < 1:297302/98‑1 (MQ=255)
cgAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGgc > 1:474616/1‑126 (MQ=255)
cgAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGgc < 2:474616/126‑1 (MQ=255)
cgacgaTGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGc < 1:249358/139‑1 (MQ=255)
tACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTgcg < 2:1505868/139‑1 (MQ=255)
aCGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTa > 1:1279148/1‑95 (MQ=255)
aCGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTa < 2:1279148/95‑1 (MQ=255)
aCGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTgcgc > 1:544296/1‑139 (MQ=255)
gATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGc > 1:556131/1‑58 (MQ=255)
gATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGc < 2:556131/58‑1 (MQ=255)
gATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTgcgc < 1:1309716/84‑1 (MQ=255)
gATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTgcgc > 2:1309716/1‑84 (MQ=255)
ggCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGAt > 1:1343489/1‑81 (MQ=255)
ggCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGAt < 2:1343489/81‑1 (MQ=255)
ccAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGt < 2:544296/139‑1 (MQ=255)
ccAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGt < 1:898440/139‑1 (MQ=255)
ccAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGt < 1:634484/139‑1 (MQ=255)
aGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTc < 2:1650859/100‑1 (MQ=255)
aGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTc > 1:1650859/1‑100 (MQ=255)
ggCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt > 1:1280435/1‑56 (MQ=255)
ggCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGAt < 2:1280435/56‑1 (MQ=255)
ggCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGa < 1:857152/77‑1 (MQ=255)
ggCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGa > 2:857152/1‑77 (MQ=255)
cgcAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCa < 1:1079346/139‑1 (MQ=255)
aTGCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCg > 1:1049174/1‑100 (MQ=255)
aTGCCACGCAACCGCCGTGGACAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCg < 2:1049174/100‑1 (MQ=255)
gCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCaa > 2:128349/1‑121 (MQ=255)
gCCACGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCaa < 1:128349/121‑1 (MQ=255)
cGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTc > 1:654491/1‑73 (MQ=255)
cGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTc < 2:654491/73‑1 (MQ=255)
cGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTa < 1:736491/81‑1 (MQ=255)
cGCAACCGCCGTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTa > 2:736491/1‑81 (MQ=255)
gTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATggg > 2:1682427/1‑111 (MQ=255)
gTGGGCAACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATggg < 1:1682427/111‑1 (MQ=255)
|
GCGGAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAACGACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGATGAATTACCGGCGCTGGTTCAGTCGCAATTTAATGTCAGCGACCGCTGCGCCATTAGCGGTCACTCAATGGG > NC_000913/2244117‑2244349
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
