Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I2 R1 774 49.2 2112398 96.9% 2046913 118.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,266,013 T→C R188R (CGT→CGC)  yeiP → elongation factor P‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,266,0130TC100.0% 235.4 / NA 65R188R (CGT→CGC) yeiPelongation factor P‑like protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/27);  total (38/27)

AAAGGGGCATCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTC  >  NC_000913/2265882‑2266148
                                                                                                                                   |                                                                                                                                       
aaaGGGGCATCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACt                                                                                                                                  >  2:8809/1‑139 (MQ=255)
         tCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACt                                                                                                                                  <  2:299122/130‑1 (MQ=255)
         tCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACt                                                                                                                                  >  1:299122/1‑130 (MQ=255)
               aGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATg                                                                                                                   >  1:747084/1‑139 (MQ=255)
                                        tGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTc                                                                                          >  1:843717/1‑139 (MQ=255)
                                               tggtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCttttt                                                                                   >  2:703180/1‑139 (MQ=255)
                                                ggtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAGAAGGGATAGCTCAGGCTGTCCCtttttt                                                                                  >  1:633347/1‑139 (MQ=255)
                                                 gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGTGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                                 >  2:646878/1‑139 (MQ=255)
                                                 gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAg                                                                                                                          <  2:236465/98‑1 (MQ=255)
                                                 gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAg                                                                                                                          >  1:236465/1‑98 (MQ=255)
                                                 gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                                 >  2:246448/1‑139 (MQ=255)
                                                 gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                                 >  1:726619/1‑139 (MQ=255)
                                                 gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                                 <  2:747084/139‑1 (MQ=255)
                                                  tctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTaa                                                                                <  1:8809/139‑1 (MQ=255)
                                                           tCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATtata                                                                       >  2:773499/1‑139 (MQ=255)
                                                            cAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGTGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAc                                                                      <  2:604602/139‑1 (MQ=255)
                                                               gTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                         >  1:481522/1‑101 (MQ=255)
                                                               gTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                         <  2:481522/101‑1 (MQ=255)
                                                                tACCAGAATACTTAAGCCCGGGCGAAATAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAAt                                                                  >  2:629201/1‑139 (MQ=255)
                                                                   cAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGc                                                                                                                  <  2:612176/88‑1 (MQ=255)
                                                                   cAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGc                                                                                                                  >  1:612176/1‑88 (MQ=255)
                                                                   cAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGc                                                                                                                  >  1:899497/1‑88 (MQ=255)
                                                                   cAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGc                                                                                                                  <  2:899497/88‑1 (MQ=255)
                                                                     gAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAAt                                                                               >  2:62819/1‑121 (MQ=255)
                                                                     gAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAAt                                                                               <  1:62819/121‑1 (MQ=255)
                                                                     gAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAAtcttct                                                             >  2:514500/1‑139 (MQ=255)
                                                                             aaGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAAttt                                                                             >  2:1039861/1‑115 (MQ=255)
                                                                             aaGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAAttt                                                                             <  1:1039861/115‑1 (MQ=255)
                                                                                cccGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGaaa                                                                                                             >  2:638639/1‑80 (MQ=255)
                                                                                cccGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGaaa                                                                                                             <  1:638639/80‑1 (MQ=255)
                                                                                cccGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCtt                                                  >  2:408400/1‑139 (MQ=255)
                                                                                  cGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTcc                                                >  2:215018/1‑139 (MQ=255)
                                                                                   gggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCt                                               >  2:256100/1‑139 (MQ=255)
                                                                                    ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGgcgcgc                                                                                                                                       >  2:837012/1‑50 (MQ=255)
                                                                                    ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCAt                                                                                                                    >  2:161836/1‑69 (MQ=255)
                                                                                    ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCAt                                                                                                                    <  1:161836/69‑1 (MQ=255)
                                                                                    ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                         >  2:837304/1‑80 (MQ=255)
                                                                                    ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                         <  2:17134/80‑1 (MQ=255)
                                                                                    ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                         >  1:17134/1‑80 (MQ=255)
                                                                                    ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                         <  1:837304/80‑1 (MQ=255)
                                                                                    ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCt                                                                                                   <  1:594133/86‑1 (MQ=255)
                                                                                    ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCt                                                                                                   >  2:594133/1‑86 (MQ=255)
                                                                                    ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                                 >  2:288896/1‑104 (MQ=255)
                                                                                    ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                                 <  2:16484/104‑1 (MQ=255)
                                                                                    ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                                 >  1:16484/1‑104 (MQ=255)
                                                                                    ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                                 <  1:288896/104‑1 (MQ=255)
                                                                                    ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTg                                              >  2:55917/1‑139 (MQ=255)
                                                                                    ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTg                                              >  1:797917/1‑139 (MQ=255)
                                                                                     gCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGa                                             >  1:886978/1‑139 (MQ=255)
                                                                                     gCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGa                                             <  2:792549/139‑1 (MQ=255)
                                                                                      cGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGaa                                            <  1:246448/139‑1 (MQ=255)
                                                                                       gAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAac                                           <  2:633347/139‑1 (MQ=255)
                                                                                                          tCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAAtttt                        <  2:1031465/139‑1 (MQ=255)
                                                                                                                 aCGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGA‑AGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGtt                >  1:68440/1‑139 (MQ=255)
                                                                                                                  cGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGaa                                                                                                              >  1:714023/1‑45 (MQ=255)
                                                                                                                  cGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGaa                                                                                                              <  2:714023/45‑1 (MQ=255)
                                                                                                                  cGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCg                  <  2:740998/137‑1 (MQ=255)
                                                                                                                  cGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCg                  >  1:740998/1‑137 (MQ=255)
                                                                                                                   gCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGttg               >  1:85165/1‑139 (MQ=255)
                                                                                                                       ttctcagggtCGCGCTGACTAACTTCCGCCGCATGCAGAAAAGGGATAGATCCGGCTGTACCTTTTTTAATTTATTATACAAATGTTCTATTTTGCGCTTCCTGGACACCTTCCGCGCTCAATTTTCAGTCGttgattg           >  2:418399/7‑139 (MQ=255)
                                                                                                                       ttATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGttgattg           >  2:647781/1‑139 (MQ=255)
                                                                                                                         atatGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCACTTTTCACTCGTtgattgat         <  1:703180/139‑1 (MQ=255)
                                                                                                                         atatGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTtgattgat         <  2:797917/139‑1 (MQ=255)
                                                                                                                         atatGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTtgattgat         <  1:256100/139‑1 (MQ=255)
                                                                                                                               ggCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCt   >  2:180381/1‑139 (MQ=255)
                                                                                                                               ggCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCt   <  1:904357/139‑1 (MQ=255)
                                                                                                                                gcgcgcTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTc  >  2:996225/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                       
AAAGGGGCATCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGCCCTC  >  NC_000913/2265882‑2266148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A