Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,266,013	T→C	R188R (CGT→CGC)	yeiP →	elongation factor P‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,266,013	0	T	C	100.0%	226.2 / NA	62	R188R (CGT→CGC)	yeiP	elongation factor P‑like protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (31/31); total (31/31)

GGCATCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGAT  >  NC_000913/2265887‑2266141

ggCATCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACtt                                                                                                                      >  2:358930/1‑139 (MQ=255)

    tCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGc                                                                                                                  >  2:507862/1‑139 (MQ=255)

        ccAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCa                                                                                                              <  1:358930/139‑1 (MQ=255)

         cAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCAt                                                                                                             <  1:23735/139‑1 (MQ=255)

           gcgcCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGc                                                                                                           >  2:167318/1‑139 (MQ=255)

                     cAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGa                                                                                                 <  2:742598/139‑1 (MQ=255)

                     cAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGa                                                                                                 <  1:507862/139‑1 (MQ=255)

                             cGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATATTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAg                                                                                         >  2:615465/1‑139 (MQ=255)

                                          tggtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGTAGAACGCCGTTATATGGGGCGCGCGGACTAACTTCAGCAGCATGCAGAAAAGGGATCGCTCAGGCTGCCCCttttt                                                                            >  1:966507/1‑139 (MQ=255)

                                          tggactggtGATTCAGGTACAAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCttttt                                                                            >  2:685588/1‑139 (MQ=255)

                                            gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                          >  2:726557/1‑139 (MQ=255)

                                            gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                          <  2:305204/139‑1 (MQ=255)

                                                      tCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGa                                                                                                                             <  2:724357/78‑1 (MQ=255)

                                                      tCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGa                                                                                                                             >  1:724357/1‑78 (MQ=255)

                                                      tCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATtata                                                                >  1:997408/1‑139 (MQ=255)

                                                       cAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAc                                                               <  1:120452/139‑1 (MQ=255)

                                                       cAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAc                                                               <  1:41516/139‑1 (MQ=255)

                                                                      ttAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTg                                                >  2:335159/1‑139 (MQ=255)

                                                                        aaGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTgcg                                              >  2:763043/1‑139 (MQ=255)

                                                                        aaGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTgcg                                              >  1:1009198/1‑139 (MQ=255)

                                                                         aGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTgcgc                                             <  1:61777/139‑1 (MQ=255)

                                                                           cccGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTgcgc                                             <  2:885150/137‑1 (MQ=255)

                                                                           cccGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTgcgc                                             >  1:885150/1‑137 (MQ=255)

                                                                            ccGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTc                                          <  1:335159/139‑1 (MQ=255)

                                                                             cGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                  >  2:168054/1‑82 (MQ=255)

                                                                             cGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                  <  1:168054/82‑1 (MQ=255)

                                                                             cGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTgcgc                                             >  2:473724/1‑135 (MQ=255)

                                                                             cGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTgcgc                                             <  1:473724/135‑1 (MQ=255)

                                                                              gggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTccc                                                                                 >  1:951780/1‑98 (MQ=255)

                                                                              gggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTccc                                                                                 <  2:951780/98‑1 (MQ=255)

                                                                               ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGaaa                                                                                                      <  1:455008/76‑1 (MQ=255)

                                                                               ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGaaa                                                                                                      >  2:455008/1‑76 (MQ=255)

                                                                               ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                  <  2:217779/80‑1 (MQ=255)

                                                                               ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                  >  1:217779/1‑80 (MQ=255)

                                                                               ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                          >  1:15736/1‑104 (MQ=255)

                                                                               ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                          <  2:15736/104‑1 (MQ=255)

                                                                               ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTgcgc                                             >  2:481471/1‑133 (MQ=255)

                                                                               ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTgcgc                                             <  1:481471/133‑1 (MQ=255)

                                                                               ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTg                                       >  2:967851/1‑139 (MQ=255)

                                                                               ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATACAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                          <  2:501226/104‑1 (MQ=255)

                                                                               ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATACAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                          >  1:501226/1‑104 (MQ=255)

                                                                                 cGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGaa                                     <  2:997408/139‑1 (MQ=255)

                                                                                 cGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGaa                                     <  1:615465/139‑1 (MQ=255)

                                                                                 cGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGaa                                     <  1:327174/139‑1 (MQ=255)

                                                                                    aaaaaTTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                          <  1:579387/99‑1 (MQ=255)

                                                                                    aaaaaTTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                          >  2:579387/1‑99 (MQ=255)

                                                                                                     tCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAAtttt                 <  1:167318/139‑1 (MQ=255)

                                                                                                        aagaaCGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTgcgc                                             <  1:1534/108‑1 (MQ=255)

                                                                                                        aagaaCGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTgcgc                                             >  2:1534/1‑108 (MQ=255)

                                                                                                             cGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAac                                    <  1:66760/112‑1 (MQ=255)

                                                                                                             cGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAac                                    >  2:66760/1‑112 (MQ=255)

                                                                                                             cGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGtt         >  1:24743/1‑139 (MQ=255)

                                                                                                                  ttATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGttgattg    >  2:302122/1‑139 (MQ=255)

                                                                                                                    atatGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTtgattgat  <  1:685588/139‑1 (MQ=255)

                                                                                                                     tatGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCa                                                             >  1:326233/1‑79 (MQ=255)

                                                                                                                     tatGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCa                                                             <  2:326233/79‑1 (MQ=255)

                                                                                                                          ggCGCGCTGACTAACTTCAGCCGCATGCGGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAAtttt                 >  2:627149/1‑118 (MQ=255)

                                                                                                                          ggCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCt                                                                                            >  1:159085/1‑43 (MQ=255)

                                                                                                                          ggCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCt                                                                                            <  2:159085/43‑1 (MQ=255)

                                                                                                                          ggCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGccc                         >  1:855820/1‑110 (MQ=255)

                                                                                                                          ggCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGccc                         <  2:855820/110‑1 (MQ=255)

                                                                                                                          ggCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAAtttt                 <  1:627149/118‑1 (MQ=255)

GGCATCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGAT  >  NC_000913/2265887‑2266141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A