Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I2 R1 774 49.2 2112398 96.9% 2046913 118.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,935,917 G→A G112S (GGC→AGC)  fucI → L‑fucose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,935,9170GA100.0% 68.0 / NA 22G112S (GGC→AGC) fucIL‑fucose isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/9);  total (13/9)

CGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACA  >  NC_000913/2935796‑2936030
                                                                                                                         |                                                                                                                  
cGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGgc                                                                                                  <  1:921898/139‑1 (MQ=255)
                            ggCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAg                                                                      >  2:926210/1‑139 (MQ=255)
                            ggCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAg                                                                      >  2:111409/1‑139 (MQ=255)
                              ccTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTCGCAGCGGCTCTGGCAGCt                                                                    <  2:585377/139‑1 (MQ=255)
                                tCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAg                                                                                  <  2:1029118/123‑1 (MQ=255)
                                tCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAg                                                                                  >  1:1029118/1‑123 (MQ=255)
                                          ggTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGaaa                                                        <  2:129205/139‑1 (MQ=255)
                                              aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCa                                                    >  1:981482/1‑139 (MQ=255)
                                                      cTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCa                                            <  2:981482/139‑1 (MQ=255)
                                                                ggCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCAttt                                  >  2:225484/1‑139 (MQ=255)
                                                                     tGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACgg                              >  1:409652/1‑139 (MQ=255)
                                                                     tGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTa                                 >  2:400079/1‑135 (MQ=255)
                                                                     tGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTa                                 <  1:400079/135‑1 (MQ=255)
                                                                             tCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATcc                                                 >  1:784843/1‑111 (MQ=255)
                                                                             tCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATcc                                                 <  2:784843/111‑1 (MQ=255)
                                                                                  aTGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAgg                <  2:242359/139‑1 (MQ=255)
                                                                                       tCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGcc           >  2:307318/1‑139 (MQ=255)
                                                                                           aCCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATg       >  1:649213/1‑139 (MQ=255)
                                                                                                cccgAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCAt                                           <  2:995249/98‑1 (MQ=255)
                                                                                                cccgAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCAt                                           >  1:995249/1‑98 (MQ=255)
                                                                                                cccgAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGAcaca  >  2:989822/1‑139 (MQ=255)
                                                                                                cccgAAGGCCATTTGGGGCTTTAACAGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGAcaca  >  1:1051390/1‑139 (MQ=255)
                                                                                                                         |                                                                                                                  
CGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACG‑CCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACA  >  NC_000913/2935796‑2936030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A