Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,993,856	G→A	73 (TAG→TAA)	ygeI →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,993,856	0	G	A	100.0%	206.9 / NA	64	73 (TAG→TAA)	ygeI	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (34/30); total (34/30)

ACATCAACACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTT  >  NC_000913/2993726‑2993989

acaTCAACACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGa                                                                                                                               <  2:384886/139‑1 (MQ=255)

 caTCAACACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGAc                                                                                                                              <  2:611951/139‑1 (MQ=255)

    cAACACCAGTCCTACGGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATCATATGTATTCAGAGGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCaa                                                                                                                           >  2:704920/1‑139 (MQ=255)

     aaCACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCaaa                                                                                                                          >  2:785732/1‑139 (MQ=255)

            gTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCa                                                                                                                   >  1:206455/1‑139 (MQ=255)

                  cTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACga                                                                                                             >  1:270023/1‑139 (MQ=255)

                     aTACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGAt                                                                                                          <  2:511983/139‑1 (MQ=255)

                       aCAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAAGGAGATAc                                                                                                        >  1:835508/1‑139 (MQ=255)

                       aCAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACgaga                                                                                                           <  2:360323/136‑1 (MQ=255)

                       aCAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACgaga                                                                                                           >  1:360323/1‑136 (MQ=255)

                           aTCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGAt                                                                                                          <  2:568375/133‑1 (MQ=255)

                           aTCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGAt                                                                                                          >  1:568375/1‑133 (MQ=255)

                           aTCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACtttt                                                                                                    >  1:1009022/1‑139 (MQ=255)

                           aTCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACtttt                                                                                                    >  1:726172/1‑139 (MQ=255)

                           aTCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACt                                                                                                       >  1:744993/1‑136 (MQ=255)

                           aTCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACt                                                                                                       <  2:744993/136‑1 (MQ=255)

                            tCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGAc                                                                                                                              <  1:431131/112‑1 (MQ=255)

                            tCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGAc                                                                                                                              >  2:431131/1‑112 (MQ=255)

                               aaTACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACgag                                                                                                            <  1:783621/127‑1 (MQ=255)

                               aaTACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACgag                                                                                                            >  2:783621/1‑127 (MQ=255)

                               aaTACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTta                                                                                                   >  1:7170/1‑136 (MQ=255)

                               aaTACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTta                                                                                                   <  2:7170/136‑1 (MQ=255)

                                aTACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTataat                                                                                               <  1:499108/139‑1 (MQ=255)

                                 tACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTataata                                                                                              <  1:785732/139‑1 (MQ=255)

                                     aGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTAt                                                                                          >  2:183804/1‑139 (MQ=255)

                                     aGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTAt                                                                                          >  2:411268/1‑139 (MQ=255)

                                         tttATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCg                                                                                      <  1:124252/139‑1 (MQ=255)

                                               gTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCtt                                                                                <  2:222766/139‑1 (MQ=255)

                                                 ttCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATaa                                                                                                                >  2:375020/1‑105 (MQ=255)

                                                 ttCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATaa                                                                                                                <  1:375020/105‑1 (MQ=255)

                                                 ttCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACgaga                                                                                                           <  1:1002280/110‑1 (MQ=255)

                                                 ttCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACgaga                                                                                                           >  2:1002280/1‑110 (MQ=255)

                                                  tCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATAc                                                                                                        >  1:261482/1‑112 (MQ=255)

                                                  tCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATAc                                                                                                        <  2:261482/112‑1 (MQ=255)

                                                     aCATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcattc                                                                          <  1:753101/139‑1 (MQ=255)

                                                          gatggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCaaat                                                                                                                         >  1:399520/1‑87 (MQ=255)

                                                          gatggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCaaat                                                                                                                         <  2:399520/87‑1 (MQ=255)

                                                          gatggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATcc                                                                     >  1:334183/1‑139 (MQ=255)

                                                              gcgcCTTTTCAAAATCATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTCGTAAACGATTGACCAAATAACTCATAACGAGATACTTTTATAATCGTATCTCGTTCCTTTCATTCAATCCTcac                                                                 <  1:704920/139‑1 (MQ=255)

                                                                  ctcttCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAACCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACAt                                                             <  2:698030/136‑1 (MQ=255)

                                                                                        agaagaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCaaa                                       >  1:445437/1‑139 (MQ=255)

                                                                                             aTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                                  >  2:561549/1‑139 (MQ=255)

                                                                                             aTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                                  <  2:334183/139‑1 (MQ=255)

                                                                                             aTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                                  <  1:183804/139‑1 (MQ=255)

                                                                                                       ctcACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGt                                                                                            >  2:535679/1‑71 (MQ=255)

                                                                                                       ctcACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGt                                                                                            <  1:535679/71‑1 (MQ=255)

                                                                                                         cACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCa                      >  2:1018973/1‑139 (MQ=255)

                                                                                                          aCCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCAt                     >  1:275260/1‑139 (MQ=255)

                                                                                                            cTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcatt                                                                           <  2:739198/83‑1 (MQ=255)

                                                                                                            cTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcatt                                                                           >  1:739198/1‑83 (MQ=255)

                                                                                                               aCTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTataata                                                                                              <  2:677751/61‑1 (MQ=255)

                                                                                                               aCTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTataata                                                                                              >  1:677751/1‑61 (MQ=255)

                                                                                                               aCTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                                  <  1:253529/121‑1 (MQ=255)

                                                                                                               aCTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                                  >  2:253529/1‑121 (MQ=255)

                                                                                                                cTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCAc               <  1:122353/139‑1 (MQ=255)

                                                                                                                cTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCAc               >  2:382949/1‑139 (MQ=255)

                                                                                                                 tataAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACg              <  1:561549/139‑1 (MQ=255)

                                                                                                                         tGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCAc               <  2:543328/130‑1 (MQ=255)

                                                                                                                         tGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCAc               >  1:543328/1‑130 (MQ=255)

                                                                                                                           aGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTatat    >  1:866683/1‑139 (MQ=255)

                                                                                                                           aGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATAAGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTatat    >  1:388218/1‑139 (MQ=255)

                                                                                                                             tGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTAtattt  >  1:988695/1‑139 (MQ=255)

                                                                                                                               gTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAACTATGCGCTCACCAAAATTTa                                  <  1:716831/105‑1 (MQ=255)

                                                                                                                               gTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                                  >  2:716831/1‑105 (MQ=255)

ACATCAACACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATATTT  >  NC_000913/2993726‑2993989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A