Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	748	32.0	1685426	92.6%	1560704	103.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,993,856	G→A	73 (TAG→TAA)	ygeI →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,993,856	0	G	A	100.0%	88.3 / NA	29	73 (TAG→TAA)	ygeI	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/13); total (16/13)

CACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTAT  >  NC_000913/2993733‑2993985

cacCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCaaata                                                                                                                    <  1:66155/139‑1 (MQ=255)

       gCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATa                                                                                                             >  2:429677/1‑139 (MQ=255)

               tACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATa                                                                                                     <  1:762161/139‑1 (MQ=255)

                aCAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATAc                                                                                                    <  1:429677/139‑1 (MQ=255)

                    aTCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACtttt                                                                                                >  2:51623/1‑139 (MQ=255)

                         aTACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTctaat                                                                                           >  2:208664/1‑139 (MQ=255)

                           aCAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGTTATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAg                                                                                         >  2:252622/1‑139 (MQ=255)

                                   ttATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGt                                                                                 >  2:82541/1‑139 (MQ=255)

                                           tCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAAATAATCCTATCTCACCTTACTATAAAATTGAGAGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttca                                                                         >  2:589736/1‑139 (MQ=255)

                                             aaCATGGACGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGCTAAACGATTGACCAAATAAATCATAAAGAGATACTTTTATAATAGTATCTCGTTCCTttcatt                                                                       <  2:545135/139‑1 (MQ=255)

                                              aCATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcattc                                                                      <  1:175232/139‑1 (MQ=255)

                                                       gcgcCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCaaat                                                                                                                     <  2:370025/83‑1 (MQ=255)

                                                       gcgcCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCaaat                                                                                                                     >  1:370025/1‑83 (MQ=255)

                                                                       atatGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATaa                                                                                                            <  1:691378/76‑1 (MQ=255)

                                                                       atatGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATaa                                                                                                            >  2:691378/1‑76 (MQ=255)

                                                                               tCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcatt                                                                       >  2:127873/1‑105 (MQ=255)

                                                                               tCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcatt                                                                       <  1:127873/105‑1 (MQ=255)

                                                                                     gaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGtt                               <  1:51623/139‑1 (MQ=255)

                                                                                     gaTAATCCTAACTCACCTTACTATAAAATTGAGTGGTAAACGATTGATCAAATAAATAATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGtt                               <  1:283729/139‑1 (MQ=255)

                                                                                      aTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCaa                                    <  2:834633/133‑1 (MQ=255)

                                                                                      aTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCaa                                    >  1:834633/1‑133 (MQ=255)

                                                                                      aTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                              >  2:180811/1‑139 (MQ=255)

                                                                                      aTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                              >  1:679170/1‑139 (MQ=255)

                                                                                        aaTCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAAACATAACGAGATACTTTta                                                                                               <  2:474439/72‑1 (MQ=255)

                                                                                        aaTCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAAACATAACGAGATACTTTta                                                                                               >  1:474439/1‑72 (MQ=255)

                                                                                                   aCCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACgag                                                                                                        >  2:350182/1‑52 (MQ=255)

                                                                                                   aCCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACgag                                                                                                        <  1:350182/52‑1 (MQ=255)

                                                                                                   aCCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAAAGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCAt                 >  2:667695/1‑139 (MQ=255)

                                                                                                                  tGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGtat  >  2:358813/1‑139 (MQ=255)

CACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTAT  >  NC_000913/2993733‑2993985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A