Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I2 R1
|
774 |
49.2 |
2112398 |
96.9% |
2046913 |
118.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,129,619 |
C→T |
G316G (GGC→GGT) |
metB → |
cystathionine gamma‑synthase, PLP‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,129,619 | 0 | C | T | 100.0%
| 90.3
/ NA
| 29 | G316G (GGC→GGT) | metB | cystathionine gamma‑synthase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (11/18); total (11/18) |
AAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGAC > NC_000913/4129489‑4129744
|
aaaaaCTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTc < 2:145546/139‑1 (MQ=255)
gTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGttt > 2:308456/1‑139 (MQ=255)
tcgtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCgg < 2:68714/139‑1 (MQ=255)
tcgtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCgg < 2:391440/139‑1 (MQ=255)
cgtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGa > 1:105683/1‑139 (MQ=255)
cgtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGa < 2:29408/139‑1 (MQ=255)
cgtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCCTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGa > 1:643440/1‑139 (MQ=255)
gtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGaa < 1:207764/139‑1 (MQ=255)
gtTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGaa < 1:379825/139‑1 (MQ=255)
tCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGa < 2:190978/139‑1 (MQ=255)
aGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGt > 2:203463/1‑139 (MQ=255)
gggCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGACTgg < 1:906201/139‑1 (MQ=255)
cATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGaaa > 1:963896/1‑139 (MQ=255)
cAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCAcgccg < 2:105683/139‑1 (MQ=255)
aaaaaGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAAc < 2:643440/139‑1 (MQ=255)
gCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGac < 1:367202/139‑1 (MQ=255)
gCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGac < 1:308456/139‑1 (MQ=255)
tGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCg < 1:387854/52‑1 (MQ=255)
tGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCg > 2:387854/1‑52 (MQ=255)
tGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAggggg > 2:862381/1‑81 (MQ=255)
tGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAggggg < 1:862381/81‑1 (MQ=255)
tGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTa > 1:442289/1‑94 (MQ=255)
tGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTa < 2:442289/94‑1 (MQ=255)
gATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTc > 2:289477/1‑44 (MQ=255)
gATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTc < 1:289477/44‑1 (MQ=255)
aTGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGtgc < 2:888766/139‑1 (MQ=255)
aGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATgg > 2:487649/1‑114 (MQ=255)
aGCAGACGCTGCGTCGTTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATgg < 1:487649/114‑1 (MQ=255)
gtcgtTTCCTGGGTGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGAc > 2:328053/1‑139 (MQ=255)
|
AAAAACTGTATCACCCGTCGTTGCCGGAAAATCAGGGGCATGAAATTGCCGCGCGCCAGCAAAAAGGCTTTGGCGCAATGTTGAGTTTTGAACTGGATGGCGATGAGCAGACGCTGCGTCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGAC > NC_000913/4129489‑4129744
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
